We narrowed to 19,160 results for: Tec;

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pAAV.Ple155-CI-mRuby2-W3SL_BbsI(GGA)

Plasmid

#231364

Purpose

Ple155-driven mRuby2, containing cassette for BbsI-based Golden Gate assembly (e.g. of sgRNA cassette(s)). Can be used as 'no guide' control.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

mRuby2

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-mA3-BE3

Plasmid

#113419

Purpose

Expresses mA3-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

mA3-BE3 (Apobec3 S. pyogenes and Bacteriophage PBS2, Mouse)

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA_B2AR-s-pep

Plasmid

#47438

Purpose

FRET sensor for beta2-adrenergic receptor

Depositor

Sivaraj Sivaramakrishnan

Article

Malik et al J Biol Chem. 2013 Jun 14;288(24):17167-7

Insert

beta2-adrenergic receptor (ADRB2 Human)

Tags

10 nm ER/K helix, HA, mCerulean, mCitrine, and s-…

Expression

Mammalian

Promoter

pCMV

Available Since

Sept. 11, 2013

Availability

Academic Institutions and Nonprofits only
pJS647

Plasmid

#183176

Purpose

PYR1 mutagenesis plasmid compatible with pJS646 Y2H vector

Depositor

Timothy Whitehead

Article

Beltran et al Nat Biotechnol. 2022 Jun 20. pii: 10.103

Insert

PYR1 (PYR1 Mustard Weed)

Expression

Bacterial

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HaloEKAR2.2-T2A-EGFP

Plasmid

#216495

Purpose

Expression of chemigenetic ERK biosensor (low basal brightness, extremly high dynamic range) and EGFP transfection marker in mammalian cells

Depositor

Jin Zhang

Article

Frei et al Nat Biotechnol. 2025 Apr 21. doi: 10.103

Insert

HaloEKAR2.2

Tags

T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-DDX58-ts3

Plasmid

#174243

Purpose

DDX58 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX58 shRNA (RIGI Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

Plasmid

#123353

Purpose

Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-Cameleon

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-EE-UdgX-nCas9-UdgX

Plasmid

#163566

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-EE-UdgX-nCas9-UdgX

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)EE (R126E, R132E)

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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SFFV-SMASH-CTERM-GFP

Plasmid

#185766

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-mCherry-mCherry-FLARE-AKAR

Plasmid

#123333

Purpose

Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

mCherry-mCherry-FLARE-AKAR

Tags

6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

Expression

Mammalian

Promoter

CMV

Available Since

July 27, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-CaMKII-iGECI-NES

Plasmid

#160424

Purpose

AAV plasmid for CaMKII-driven expression of a genetically-encoded calcium indicator iGECI with a nuclear export signal sequence

Depositor

Vladislav Verkhusha

Article

Shemetov et al Nat Biotechnol. 2021 Mar;39(3):368-377.

Insert

iGECI-NES

Use

AAV

Expression

Mammalian

Promoter

CaMKII

Available Since

Jan. 7, 2021

Availability

Academic Institutions and Nonprofits only

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SFFV-SMASH-NTERM-GFP

Plasmid

#185761

Purpose

Degron-GFP fusion plasmid with restriction sites BamH1 and EcoR1 around GFP for subcloning

Depositor

Alessandra Ianari, William Sellers

Article

Bondeson et al Nat Commun. 2022 Sep 20;13(1):5495. doi:

Insert

GFP

Use

Lentiviral

Tags

V5, SMASh

Mutation

none

Promoter

SFFV

Available Since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_APOBEC1-UdgX-NG-nCas9

Plasmid

#163533

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

APOBEC1-UdgX-NG-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)NG (L111R, D1135V, G1218R, E1219F, A1…

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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AID-BE4max-P2A-GFP

Plasmid

#157948

Purpose

Mammalian expression of AID-BE4max-P2A-GFP

Depositor

Dali Li

Article

Zhang et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

Insert

AID-BE4max

Use

CRISPR

Expression

Mammalian

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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PET-B-evoSpCas9-NLS-6xHis

Plasmid

#207390

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

B-evoSpCas9

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

M495V, Y515N, K526E, R661Q, amino acids 1005-1013…

Promoter

T7

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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A*24:07:01

Plasmid

#203283

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*24:07:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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non-targeting control gRNA (BRDN0001145694)

Plasmid

#80191

Purpose

3rd generation lentiviral gRNA plasmid, non-targeting control gRNA

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

Non-targeting control gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-HLA-cmyc-natT1R2

Plasmid

#113946

Purpose

mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

Signal/leader sequence from HLA class I histocomp…

Expression

Mammalian

Mutation

residues 22-839

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Venus-Venus-FLARE-Cameleon

Plasmid

#123351

Purpose

Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Venus-Venus-FLARE-Cameleon

Tags

6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMonAID_nCas9-PmCDA_Hyg_ALS

Plasmid

#91693

Purpose

Monocot Target-AID vector expressing rice-optimized nCas9-PmCDA1 with sgRNA targeting OsAls

Depositor

Akihiko Kondo

Article

Shimatani et al Nat Biotechnol. 2017 Mar 27. doi: 10.103

Insert

SpCas9

Tags

PmCDA1

Expression

Plant

Mutation

D10A for nickase Cas9

Promoter

2x35S

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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