We narrowed to 9,980 results for: Uty
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Plasmid#141091PurposepH-stable bioluminescent reporter that serves as the control counterpart of pHLuc; utilizes eGFP instead of SEPDepositorInsertAntares-T2A-puromycin-IRESv24-eGFP-Nanoluc
ExpressionMammalianPromoterCMVAvailable SinceMay 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_CAG_3xTurboGFP-NLS
Plasmid#229777PurposeBxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)DepositorInsert3x TurboGFP
UseSynthetic BiologyTagsNLSExpressionMammalianPromoterCAGAvailable SinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFD115
Plasmid#124768PurposepFD115 carries cas9 controlled by an aTc-inducible Ptet promoter, a sgRNA controlled constitutively from PpflB S. aureus promoter to clone a guide between two BsaI sites and oriT on pLZ12 vectorDepositorInsertsPtet
cas9
term PpflB sgRNA (BsaI)
UseCRISPRPromoterPtetAvailable SinceAug. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-FNLSHiFiNG-P2A-Puro
Plasmid#136903PurposeLentivirus for constitutive expression of FNLSHiFiNG in mammalian cells (codon optimized)DepositorInsertFNLSHiFiNG
UseLentiviralTagsFLAG-NLS and NLSMutationR691A,L1111R,D1135V,G1218R,E1219F,A1322R,R1335V,Tā¦PromoterEF1sAvailable SinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
4A3.PBAD-repL(WT).J23115-gfp
Plasmid#197872PurposeArabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expressionDepositorInsertspart kilA/repL
gfp
ExpressionBacterialMutationcontains only the 24 bp of 3'-end of kilA geā¦PromoterBba_J23115 and PBADAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
4A3.PBAD-repL(AT2).J23115-gfp
Plasmid#197873PurposeArabinose induced expression of repL(AT2) gene, which triggers DNA replication in trans, weak level of constitutive GFP expressionDepositorInsertpart kilA/repL(AT2)
ExpressionBacterialPromoterPBADAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-CMV-H2B-3xFLAG-2A-G(N2c)
Plasmid#194355PurposeAAV constitutively expressing nuclear FLAG and Rabies Glycoprotein (G) of CVS-N2c strainDepositorInsertsH2B-3xFLAG
Rabies CVS-N2c glycoprotein (G)
UseAAVTagsFLAGAvailable SinceAug. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
Burden Monitor phi80 version
Plasmid#66074PurposeThe burden monitor was cloned inside plasmid pAH153 [Haldimann2001]; the genome integration of the system allows the measurement of the burden imposed by synthetic constructs to Escherichia coli cellDepositorInsertGFP
Available SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
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L2_UBE2:mT-N7-CsA
Plasmid#136133PurposePlasmid to transform Mp with constitutive promoter driving mTurq to the nuclei. HygRDepositorInsertp5-35S:HygR p5-MpUBE2:mTurq-N7
UseSynthetic BiologyExpressionPlantMutationBsaI/ SapI domesticatedAvailable SinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pMRE161
Plasmid#118509Purposebroad-host range plasmid to constitutively express fluorescent protein genes in bacteriaDepositorInsertmTurquoise2
ExpressionBacterialAvailable SinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTSara-C
Plasmid#60722PurposeExpresses the C-terminal fragment of split T7 RNAP split a position 179. Insert is driven by the arabinose inducible promoter PBAD. Contains a constitutive araC ORFDepositorInsertResidues 180-880 of T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationOnly amino acids 180-880 of T7 RNAPPromoterPBADAvailable SinceNov. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTSara-N
Plasmid#60721PurposeExpresses the N-terminal fragment of split T7 RNAP split a position 179. Insert is driven by PBAD. Contains a constitutive araC ORFDepositorInsertResidues 1-179 of T7 RNAP
UseSynthetic BiologyExpressionBacterialMutationOnly amino acids 1-179 of T7 RNAPPromoterPBADAvailable SinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-LexAOx4-Pmin-Fluc-PGK-Rluc-P2A-mCherry
Plasmid#192634PurposeLentiviral vector for inducible Fluc expression with 4x LexA operator (LexAO) and constitutive Rluc and mCherry.DepositorInsertfirefly luciferase
UseLentiviralExpressionMammalianPromoterMinimal promoterAvailable SinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV7.1_3xFLAG-WWC3
Plasmid#172996Purposeconstitutive expression of FLAG-tagged WWC3DepositorAvailable SinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_(GGS)6_SNIPR
Plasmid#188344PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a (GGS)6, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_(GGS)6_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_PGK_SNIPR_FnIII-Notch
Plasmid#188342PurposeLentiviral vector - constitutive expression of an anti-CD19 SNIPR with a FnIII-ECD, a Notch1-TMD, a Notch1-JMD, and a Gal4VP64 transcriptional factorDepositorInsertPGK_antiCD19_FnIII-ECD_Notch1-TMD_Notch1-JMD_Gal4VP64
UseLentiviralAvailable SinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCD5-D/bovine/Nebraska/9-5/2012-HEFwtED-GCN4-sfGFP-ST
Plasmid#175019PurposeExpresses influenza D virus trimeric hemagglutinin esterase fusion protein fused to a sfGFPDepositorInsertD/bovine/Nebraska/9-5/2012
TagsGCN4-TEV-sfGFP-TwinStrepExpressionMammalianPromoterCMVAvailable SinceJan. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_CAG-TurboGFP
Plasmid#229781PurposeBxb1-GA donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFPDepositorInsertTurboGFP
UseSynthetic BiologyTagsNLSExpressionMammalianPromoterCAGAvailable SinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only