We narrowed to 12,152 results for: 112

1. pG4-CMV-luc

   Plasmid

   #46322

   Depositor

   Steven Brown

   Article

   Kowalska et al Mol Cell Biol. 2012 Nov;32(22):4585-94.

   Insert

   5xGal4
   

   Use

   Luciferase

   Tags

   CMV promoter and luciferase

   Expression

   Mammalian

   Mutation

   five copies of a multimerized GAL4 (5×GAL4) site …

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

2. ZJOM156

   Plasmid

   #133636

   Purpose

   Integration of 2xGFP tag at SEC7 C terminus, use auxotrophic marker URA3(Candida albicans). Marker for yeast late golgi/early endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   SEC7-2GFP (SEC7 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Feb. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCFJ150-Cas9(dpiRNA)

   Plasmid

   #107940

   Purpose

   eft-3p::Cas9(dpiRNA)::tbb-2 3'UTR construct used for MosSCI in nematode. Cas9 is optimized by removing all piRNA targeting sites to allow germline expression.

   Depositor

   Heng-Chi Lee

   Article

   Zhang et al Science. 2018 Feb 2;359(6375):587-592. d

   Insert

   Cas9(dpiRNA)
   

   Expression

   Worm

   Promoter

   eft-3

   Available Since

   April 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHelper-CBE-sgRNA

   Plasmid

   #221137

   Purpose

   Helper plasmid for sgRNA cloning for CRISPR-Cas9 cytosine base editing. sgRNA-scaffold with Cas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

   Depositor

   Craig Roy

   Article

   Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

   Insert

   sgRNA-scaffold with Cas9 handle
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   J23119(SpeI)

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SG3ΔENV CA Q63A,Q67A

   Plasmid

   #145784

   Purpose

   Non-infectious HIV-1 Clone with stop codon in Env (see NIH AIDS Reagent Program Cat #11051) and Q63A,Q67A mutation in capsid (CA Q63A,Q67A).

   Depositor

   Wesley Sundquist

   Article

   Christensen et al Science. 2020 Oct 9;370(6513). pii: 370/

   Insert

   Capsid
   

   Use

   Lentiviral and Retroviral

   Expression

   Mammalian

   Mutation

   Changed Capsid residues Glutamines 63 and 67 to a…

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Pb459-mU6-sgRNA-EF1a-PuroR

   Plasmid

   #195507

   Purpose

   piggybac vector expressing non-targeting control sgRNA cloned using BlpI and BstXI sites

   Depositor

   Joanna Wysocka

   Article

   Bajpai et al Science. 2023 Aug 11;381(6658):eade6289.

   Insert

   Puromycin
   

   Use

   CRISPR; Piggybac

   Expression

   Mammalian

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pTorPE-G-GECO1.2

   Plasmid

   #32468

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   G-GECO1.2
   

   Use

   Lab constructed

   Tags

   6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

   Expression

   Bacterial

   Mutation

   GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

   Available Since

   Sept. 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJM195A

   Plasmid

   #218125

   Purpose

   Hybrid circuit displaying hsCRBN-CTD bait and driving expression of gIII

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Inserts

   CRBN_CTD
   M13 pIII
   

   Tags

   434cI_RR69

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJM194A

   Plasmid

   #218124

   Purpose

   Hybrid circuit displaying hsCRBN-CTD bait and driving expression of luxAB

   Depositor

   David Liu

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Inserts

   CRBN_CTD
   luxAB
   

   Tags

   434cI_RR69

   Available Since

   June 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLV-RhoB Sensor-IRES-Blast

    Plasmid

    #222633

    Purpose

    Rho reporter

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    RhoB Sensor
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Sept. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. C1-FT-mCitrine-EAAARx8-CaaX

    Plasmid

    #155224

    Purpose

    MPAct with increased search radius

    Depositor

    Tobias Meyer

    Article

    Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

    Insert

    F-tractin (aa9-52 of rat ITPKA) fused to mCitrine C-term tagged with codon optimized (EAAAR)x8 and CaaX seq derived from Kras4b
    

    Tags

    mCitrine

    Expression

    Mammalian

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDARMO_CMVT_3xFLAG_BIRC6_BIRmut

    Plasmid

    #197968

    Purpose

    Mammalian cell expression of 3xFLAG-BIRC6_BIRmut

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    C328S, C331S, L1332V

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pNP1 eforRed toehold switch

    Plasmid

    #107353

    Purpose

    Expresses eforRed (an RFP derivative) in Ecoli off a T7 promoter when pNP1 eforRed trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 1 + eforRed
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. mOtop1_pGEMHE

    Plasmid

    #114674

    Purpose

    This construct was used to express mouse Otop1 in Xenopus laevis oocytes

    Depositor

    Emily Liman

    Article

    Tu et al Science. 2018 Mar 2;359(6379):1047-1050.

    Insert

    Otopetrin 1 (Otop1 Mouse)
    

    Use

    For generating the mrna, which can highly express…

    Mutation

    SNP (rs49325632): Glycine 396 to Alanine

    Promoter

    T7

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. ZJOM25

    Plasmid

    #133672

    Purpose

    Integration of MSP1-2GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Present on mitochondria and peroxisomes.

    Depositor

    Zhiping Xie

    Article

    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

    Insert

    p1k-MSP1-2GFP (MSP1 Budding Yeast)
    

    Tags

    EGFP

    Expression

    Yeast

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJM160

    Plasmid

    #134169

    Purpose

    Bacterial expression of RB3 stathmin-like domain (SLD)

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    RB3-SLD (STMN4 Human)
    

    Expression

    Bacterial

    Mutation

    residues 49-189

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiEKAR2G1

    Plasmid

    #40177

    Depositor

    Olivier Pertz

    Article

    Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

    Insert

    Erk activity reporter
    

    Use

    Lentiviral

    Tags

    His-Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. Flag Nprl2 pLJM1

    Plasmid

    #46338

    Depositor

    David Sabatini

    Article

    Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

    Insert

    Nprl2 (NPRL2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    July 15, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pJL1-MBP-4xDQNAT

    Plasmid

    #128390

    Purpose

    In vitro expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Maltose binding protein
    

    Tags

    4xDQNAT glycosylation tag and 6xHis tag

    Expression

    Bacterial

    Mutation

    A302T- please see depositor comment

    Promoter

    T7

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDARMO_CMVT_N_Strep_BIRC6

    Plasmid

    #197972

    Purpose

    Mammalian cell expression of wt Strep-BIRC6

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Tags

    Strep

    Expression

    Mammalian

    Mutation

    L1332V

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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