169,603 results

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pAAV-GFP

Plasmid

#32395

Depositor

John T Gray

Article

Gray et al Methods Mol Biol. 2011;807:25-46.

Insert

GFP

Use

AAV; Adeno-associated virus

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2011

Availability

Academic Institutions and Nonprofits only

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pHelper_ShCAST_sgRNA

Plasmid

#127921

Purpose

Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

ShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)

Available Since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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TFORF2069

Plasmid

#141902

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP V5

Plasmid

#59758

Purpose

encodes multiple copies of an epitope tag V5

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _V5

Expression

Mammalian

Promoter

CAG

Available Since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pHIV-IL13-IgG4hinge-PDGFR

Plasmid

#240245

Purpose

Lentiviral transfer plasmid encoding IL13 with IgG4 hinge and PDGFR TM domain

Depositor

Michael Birnbaum

Article

Dobson et al Nat Methods. 2022 Apr;19(4):449-460. doi

Insert

IL-13 (IL13 Human)

Use

Lentiviral

Tags

EGFP and HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pAG306GAL-ccdB-EGFP

Plasmid

#14187

Depositor

Susan Lindquist

Article

Alberti et al Yeast. 2007 Oct . 24(10):913-9.

Type

Empty backbone

Use

Gateway destination

Tags

EGFP

Expression

Yeast

Available Since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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HIV-1 LTR-gfp

Plasmid

#115809

Purpose

HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gfp and ngfr

Use

Lentiviral

Promoter

HIV1 LTR

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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HER2Nb-C AND (A OR T)-SpyTag

Plasmid

#243790

Purpose

Expresses multifunctional construct enabling the AND(OR)-gated release of a HER2 Nanobody from materials in response to HRV-3C AND (sortase eSrtA(2A9) OR TEV) treatment.

Depositor

Cole DeForest

Article

Gharios et al Nat Chem Biol. 2025 Oct 9. doi: 10.1038/

Insert

HER2 nanobody

Tags

6x His tag and SsrA

Expression

Bacterial

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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FU-H2B-GFP-IRES-Puro

Plasmid

#69550

Purpose

Lentiviral, selectable fluorescent GFP tagging of nuclei.

Depositor

Charles Gersbach

Insert

Histone 2B - green fluorescent protein fusion

Use

Lentiviral

Tags

GFP fusion

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag3HuR

Plasmid

#135950

Purpose

Expresses HuR in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Article

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Insert

ELAV like RNA binding protein 1 (ELAVL1 Human)

Tags

Flag3

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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CTCF-mAC donor (Hygro)

Plasmid

#140646

Purpose

CTCF tagging with mAID-clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

CTCF (CTCF Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-GFP11-His3MX

Plasmid

#91951

Purpose

Universal C-terminal GFP11 tagging

Depositor

Rong Li

Article

Ruan et al Nature. 2017 Mar 16;543(7645):443-446. d

Type

Empty backbone

Expression

Yeast

Available Since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-GFP-fGFP

Plasmid

#135631

Purpose

AAV vector to drive the expression of fGFP in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

GFP-fGFP

Use

AAV

Mutation

None

Available Since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-FLAG-Synaptojanin 1-145

Plasmid

#22293

Depositor

Pietro De Camilli

Article

Perera et al Proc Natl Acad Sci U S A. 2006 Dec 19. 1

Insert

Synaptojanin 1_145 (SYNJ1 Human)

Tags

Flag and GFP

Expression

Bacterial and Mammalian

Available Since

Oct. 21, 2009

Availability

Academic Institutions and Nonprofits only

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AAV-EFS-SaKKHABE8e-bGH-U6-sgRNA-BsmBI

Plasmid

#189923

Purpose

AAV genome encoding SaKKHABE8e and Sa sgRNA cassette on a single AAV, with BsmBI sites for protospacer installation

Depositor

David Liu

Article

Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

Insert

SaABE8e V106W

Use

AAV

Mutation

nSaCas9 KKH, TadA ABE8e V106W

Promoter

EFS

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomCherry-CaURA3

Plasmid

#44876

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mCherry with CaUra3 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomCherry

Expression

Yeast

Available Since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGP5G-ccdB

Plasmid

#83879

Purpose

Destination vector for integration at yeast Leu locus, expression from the GPD promoter

Depositor

Jennifer Nemhauser

Type

Empty backbone

Use

Gateway destination vector

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-mcherry)

Plasmid

#217306

Purpose

sgRNA mcherry + Puromycin

Depositor

Luke Gilbert

Insert

pU6-sgRNA, Ef1alpha-Puro-T2A-mcherry

Use

CRISPR

Promoter

U6, Ef1alpha

Available Since

April 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-InPGrp-Kras

Plasmid

#236001

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to plasma membrane.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

InPGrp-Kras (Cyth3 Rat, Synthetic)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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DDX21

Plasmid

#155510

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX21 (DDX21 Human)

Tags

V5-MCP

Expression

Mammalian

Mutation

T232A

Promoter

EF-1a

Available Since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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