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pLenti-Puro-sgAMPKa1

Plasmid

#138684

Purpose

Expresses a human AMPKa1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgAMPKa1 human (PRKAA1 Human)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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mcherry-Myo 9b

Plasmid

#134918

Purpose

Expression of rat Myo 9b (Myr 5) in mammalian cells.

Depositor

Martin Bähler

Article

Hemkemeyer et al J Biol Chem. 2021 Jan-Jun;296:100136. do

Insert

Myo 9b (rat) with 3' UTR (Myo9b Rat)

Tags

mcherry

Expression

Mammalian

Promoter

CMV IE

Available Since

Jan. 28, 2020

Availability

Academic Institutions and Nonprofits only

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FUT8-Dox

Plasmid

#124631

Purpose

Doxycycline-inducible FUT8 expression in mammalian cells

Depositor

Ron Weiss

Article

Chang et al Nat Chem Biol. 2019 May 20. pii: 10.1038

Inserts

Alpha-(1,6)-fucosyltransferase
Tetracycline repressor A3 mutant
TagFP635

Expression

Mammalian

Promoter

TRE-tight and hEF1a

Available Since

July 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

Plasmid

#127312

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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GBSGFP in pBS-KS

Plasmid

#121974

Purpose

Gli activity reporter

Depositor

Hiroshi Sasaki

Article

Sasaki et al Development. 1997 Apr . 124(7):1313-22.

Insert

GBSGFP (Gnb5 Mouse)

Use

Cloning

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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p2T-CAG-KKHSaCas9-BlastR

Plasmid

#107189

Purpose

Confers constitutive expression of KKH SaCas9.

Depositor

Richard Sherwood

Article

Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

Insert

KKH SaCas9 (NEWENTRY )

Expression

Bacterial

Mutation

E782K/N968K/R1015H

Promoter

Chicken ß-Actin

Available Since

Dec. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEIGW-human KLF4

Plasmid

#100131

Purpose

Human KLF4 lentiviral expression vector

Depositor

Eva Hernando

Article

Segura et al Oncotarget. 2017 Apr 18;8(16):26298-2631

Insert

Kruppel like factor 4 (KLF4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

E1F

Available Since

Feb. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-Chronos-GFP

Plasmid

#99232

Purpose

AAV-mediated expression of Chronos-GFP under the CAG promoter. Using bGHpA signal.

Depositor

Edward Boyden

Article

Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

Insert

Chronos-GFP

Use

AAV

Tags

GFP

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 2, 2017

Availability

Academic Institutions and Nonprofits only

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YEp195-CUP1-His-Smt3

Plasmid

#99538

Purpose

Yeast episomal vector for expression of His-tagged Smt3 (SUMO); URA3 marker

Depositor

Helle Ulrich

Article

Ulrich et al Methods Mol Biol. 2009;497:81-103. doi:

Insert

SMT3 (SMT3 Budding Yeast)

Tags

7xHis

Expression

Yeast

Promoter

CUP1

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-p53 2KR

Plasmid

#78799

Purpose

To overexpress p53 2KR in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

p53 (TP53 Human)

Tags

HA

Expression

Mammalian

Mutation

K291R, K292R

Promoter

CMV

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
attB-GFP-Dnmt3b1-Poly(A)

Plasmid

#65531

Purpose

Plasmid for Bxb1-mediated recombination of the GFP-Dnmt3b1 cDNA into a MIN-tagged locus

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

Dnmt3b (Dnmt3b Mouse)

Use

Mouse Targeting; Bxb1

Tags

GFP

Expression

Mammalian

Mutation

isoform 1

Available Since

Aug. 31, 2015

Availability

Academic Institutions and Nonprofits only

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myr-FLAG-PIK3CA-pcw107

Plasmid

#64605

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

PIK3CA (PIK3CA Bovine)

Use

Lentiviral

Tags

FLAG and myristoylated

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
LacI INCENP

Plasmid

#45236

Purpose

Target INCENP to lac operator sequence

Depositor

Michael Lampson

Article

Wang et al J Cell Biol. 2011 Aug 22;194(4):539-49.

Insert

LacI, INCENP-Δcen, mCherry (lacI )

Tags

LacI (lac repressor protein) and mCherry

Expression

Mammalian

Mutation

CEN box deleted from INCENP (aa1-46)

Promoter

t7

Available Since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only

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KRAS2B

Plasmid

#55655

Purpose

expression of mouse KRAS2B

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

KRAS2B (Kras Mouse)

Expression

Bacterial

Promoter

lac

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
-2.5Kb MMP10

Plasmid

#53973

Purpose

Luciferase reporter for mouse MMP10 2.5kb promoter

Depositor

Michael Chin

Article

Wu et al Biochem Biophys Res Commun. 2011 Dec 2;4

Insert

mouse MMP10 2.5Kb promoter (Mmp10 Mouse)

Use

Luciferase

Expression

Mammalian

Promoter

PCR from C57BL6/J

Available Since

June 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2 xenopus sFRP2

Plasmid

#24696

Depositor

Edward De Robertis

Article

Pera et al Mech Dev. 2000 Sep . 96(2):183-95.

Insert

secreted frizzled-related sequence protein 2 (sfrp2 Frog)

Expression

Mammalian

Available Since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
GFP-pCdc42

Plasmid

#23240

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Cdc42 (CDC42 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
M16 pCS-twhh-bGFP

Plasmid

#17153

Depositor

Randall Moon

Article

Du et al J Cell Biol. 1997 Oct 6. 139(1):145-56.

Insert

twhh promoter (shhb Zebrafish)

Tags

bGFP

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
-111 hTF m2

Plasmid

#15449

Depositor

Nigel Mackman

Article

Cui et al J Biol Chem. 1996 Feb 2. 271(5):2731-9.

Insert

Tissue factor promoter (F3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

All 3 Sp1 sites mutated

Available Since

Aug. 31, 2007

Availability

Academic Institutions and Nonprofits only
pLIA CRX (CC#69)

Plasmid

#15128

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

CRX (Crx Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

June 15, 2007

Availability

Academic Institutions and Nonprofits only