We narrowed to 13,278 results for: sequence
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Plasmid#147646PurposeMammalian Expression of HsSmg7_633-1091-siRNAresDepositorInsertHsSmg7_633-1091-siRNAres (SMG7 Human)
ExpressionMammalianMutationtwo non silent mutations Q651R and V854I comparedā¦Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pN1-HsSmg7-siRNAres-V5-SBP-MBP_S
Plasmid#147474PurposeMammalian Expression of HsSmg7-siRNAresDepositorInsertHsSmg7-siRNAres (SMG7 Human)
ExpressionMammalianMutationthree silent mutation G216A, T1185C, A1521G and oā¦Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HsSmg5-del133-155-G120E_T
Plasmid#147554PurposeMammalian Expression of HsSmg5-del133-155-G120EDepositorInsertHsSmg5-del133-155-G120E (SMG5 Human)
ExpressionMammalianMutationone silent mutation compared to the sequence giveā¦Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmHPat_57-968-dsRNAres_J
Plasmid#146690PurposeInsect Expression of DmHPat_57-968-dsRNAresDepositorInsertDmHPat_57-968-dsRNAres (Patr-1 Fly)
ExpressionInsectMutationone silent mutation A1758G and one non silent G56ā¦Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmHPat_57-968-del500-684_J
Plasmid#146695PurposeInsect Expression of DmHPat_57-968-del500-684DepositorInsertDmHPat_57-968-del500-684 (Patr-1 Fly)
ExpressionInsectMutationtwo silent mutations compared to the sequence givā¦Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmMe31B-mut2_F
Plasmid#146272PurposeInsect Expression of DmMe31B-mut2DepositorInsertDmMe31B-mut2 (me31B Fly)
ExpressionInsectMutationone silent mutation A1101G and one non silent mutā¦Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-HA-DmHPat_57-968-dsRNAres_H
Plasmid#146464PurposeInsect Expression of DmHPat_57-968-dsRNAresDepositorInsertDmHPat_57-968-dsRNAres (Patr-1 Fly)
ExpressionInsectMutationone silent mutation T477C and one non silent mutaā¦Available SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis) K223I
Plasmid#195615PurposeExpresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K223I mutation that results in nicking activityDepositorInsertI-SceI
ExpressionBacterialMutationC2694G, G2345C, G2351C, A3365T, A3366TPromoterT7Available SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET15b I-SceI (Ctermhis) K122I
Plasmid#195614PurposeExpresses I-SceI homing endonuclease from the T7 promoter containing an additional methionine-histidine sequence at the amino terminus and a K122I mutation that results in nicking activityDepositorInsertI-SceI
ExpressionBacterialMutationC2458G, A2826T, A2827TPromoterT7Available SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmGe1_J
Plasmid#146688PurposeInsect Expression of DmGe1DepositorInsertDmGe1 (Ge-1 Fly)
ExpressionInsectMutationtwo non silent mutations Q194R and I1008T compareā¦Available SinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXD70Tet(DadRmt7)-N-3xFLAG-DadR(linker)
Plasmid#191643PurposeE. coli - Eggerthella lenta shuttle plasmid (TetR), N-3xFLAG-dadR(linker region shuffled) complementationDepositorInsertDadR
Tags3xFLAGExpressionBacterialMutationLinker region sequence shuffledAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::HA-RfA
Plasmid#186404PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter HA tag and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-R4-bpFOG-L5
Plasmid#186369PurposeEntry clone with ORF encoding Strong basal promoter of FOG flanked by Gateway recombination sequencesDepositorInsertBasal promoter of the Friend Of Gata gene
UseProtein expressionPromoterT7 promoterAvailable SinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA251::PpsbA2*::B0032::CYP110D1 N-terminal His-Tag
Plasmid#186708PurposeN-terminal His-tagged CYP110D1 coding sequence under the control of PpsbA2* promoter and the BBa_B0032 RBS, for the expression in Synechocystis.DepositorInsertalr4766
UseSynthetic BiologyTagsHis-Tag (6His)PromoterPpsbA2*Available SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEVA251::Ptrc.x.tetO2::B0032::CYP110D1 N-terminal His-Tag
Plasmid#186710PurposeN-terminal His-tagged CYP110D1 coding sequence under the control of Ptrc.x.tetO2 promoter and the BBa_B0032 RBS, for the expression in Synechocystis.DepositorInsertalr4766
UseSynthetic BiologyTagsHis-Tag (6His)PromoterPtrc.x.tetO2Available SinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA
Plasmid#186407PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined gene of interest under control of an AttB3/B5 recombined reg. sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-ECFP
Plasmid#186403PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter ECFP under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-ECFP
Plasmid#186402PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::RfA-venus
Plasmid#186401PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::venus-RfA
Plasmid#186400PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorAvailable SinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only