We narrowed to 1,090 results for: rbis

1. pCMV-T7-SpCas9(SRSCMV)-P2A-EGFP (RTW3732)

   Plasmid

   #223074

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with SRSCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(SRSCMV)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(SRSCMV)=D1135S, S1136R, G1218S, E1219C, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-T7-SpCas9(MKRCMV)-P2A-EGFP (AHK162)

   Plasmid

   #223075

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKRCMV amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MKRCMV)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(MKRCMV)=D1135M, S1136K, G1218R, E1219C, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCMV-T7-SpCas9(MWNVML)-P2A-EGFP (AHK156)

   Plasmid

   #223076

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MWNVML amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MWNVML)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(MWNVML)=D1135M, S1136W, G1218N, E1219V, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCMV-T7-SpCas9(MKKCMN)-P2A-EGFP (AHK129)

   Plasmid

   #223077

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MKKCMN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MKKCMN)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(MKKCMN)=D1135M, S1136K, G1218K, E1219C, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCMV-T7-SpCas9(KKKCMN)-P2A-EGFP (AHK131)

   Plasmid

   #223078

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with KKKCMN amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KKKCMN)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(KKKCMN)=D1135K, S1136K, G1218K, E1219C, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-T7-SpCas9(MRRWMR)-P2A-EGFP (RAS2816)

   Plasmid

   #223068

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with MRRWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(MRRWMR)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(MRRWMR)=D1135M, S1136R, G1218R, E1219W, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV-T7-SpCas9(ARGIMR)-P2A-EGFP (AHK147)

   Plasmid

   #223069

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARGIMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(ARGIMR)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(ARGIMR)=D1135A, S1136R, G1218G, E1219I, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pCMV-T7-SpCas9(ARTWGR)-P2A-EGFP (RAS2995)

   Plasmid

   #223070

   Purpose

   pCMV and pT7 Human expression plasmid for SpCas9 enzyme with ARTWGR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(ARTWGR)-P2A-EGFP
   

   Use

   CRISPR

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(ARTWGR)=D1135A, S1136R, G1218T, E1219W, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCMV-T7-SpCas9(KRHWMR)-P2A-EGFP_(RAS3808)

   Plasmid

   #228251

   Purpose

   pCMV human expression plasmid for SpCas9 enzyme with KRHWMR amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

   Insert

   human codon optimized nuclease SpCas9(KRHWMR)-P2A-EGFP
   

   Use

   Tags

   BPNLS-3xFLAG-P2A-EGFP

   Expression

   Mammalian

   Mutation

   SpCas9(ARGIMR)=D1135K, S1136R, G1218H, E1219W, R1…

   Promoter

   CMV and T7

   Available since

   April 23, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMVtrunc msfGFPΔC10-Utr28-222

    Plasmid

    #231558

    Purpose

    Expresses N- and C-terminally truncated Utrophin calponin homology domain fused to C-terminally truncated msfGFP for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    Utrophin calponin homology domain (UTRN Human)
    

    Use

    Tags

    msfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMVtrunc

    Available since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLX304_zeo_mmFbxw7(res)

    Plasmid

    #160106

    Purpose

    Expresses murine Fbxw7 alpha with a T199 silent mutation in mammalian cells. The T199 silent mutation confers resistance to the 5'-TGAACATGGTACAAGGCCAG-3' sgRNA

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    Fbxw7alpha (Fbxw7 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. px330 Gatad2a sgRNA (for deletion)

    Plasmid

    #110814

    Purpose

    Expressed sgRNA for generating Gatad2a deletion (knockout ) in mouse cell lines

    Depositor

    Jacob Hanna

    Article

    Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

    Insert

    mGataD2a (Gatad2a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAGGS SARS-CoV-2 BA.4/5 Spike

    Plasmid

    #186031

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BA.4/5 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

    Insert

    SARS-CoV-2 BA.4/5 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    BA.2 mutations (T19I, LPPA24S, G142D, V213G, G339…

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    June 8, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pCDNA3-Flag-SPRTN-E112A

    Plasmid

    #110215

    Purpose

    Expression in mammalian cells of of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, and Flag-tag at N-terminus.

    Depositor

    Ivana Marinovic-Terzic, Kristijan Ramadan

    Article

    Lessel et al Nat Genet. 2014 Nov;46(11):1239-44. doi:

    Insert

    SPRTN (SPRTN Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    E112A, P296L (see depositor comments below)

    Promoter

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pCAGGS SARS-CoV-2 BQ.1.1 Spike

    Plasmid

    #193710

    Purpose

    Expresses codon-optimized full length SARS-CoV-2 BQ.1.1 Spike

    Depositor

    Marceline Côté

    Article

    Tauzin et al Cell Rep. 2023 Jan 31;42(1):111998. doi:

    Insert

    SARS-CoV-2 BQ.1.1 Spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Contains the following mutations: T19I, LPPA24S, …

    Promoter

    chicken β-actin promoter, CMV enhancer

    Available since

    Nov. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pACYC-T7-SpCas9(BspMI/SapI/BsaI_cassettes)-T7-gRNA1 (BPK1807)

    Plasmid

    #223065

    Purpose

    Dual pT7 entry plasmid for SpCas9(6AA) library; bacterial expression plasmid with type IIS RE cassettes around D1135/S1136, G1218/E1219, R1335/T1337 (precursor to MMW94). Expresses a gRNA.

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    entry vector for human codon opt. bacterial expr. plasmid for SpCas9(6AA_NNS) library, with gRNA targeting EGFP site 1
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Bacterial

    Mutation

    Three regions of SpCas9 encode type IIS restricti…

    Promoter

    Dual T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-TadCBEd-SpCas9(LWKYQS)-P2A-EGFP (BKS1014)

    Plasmid

    #223127

    Purpose

    pCMV and pT7 Human expression plasmid for TadCBEd using SpCas9 CBE enzyme with LWKYQS amino acids at positions 1135,1136,1218,1219,1335,T1337 with C-term BPNLS-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized TadA-CDd-SpCas9(LWKYQS)-2xUGI-P2A-EGFP
    

    Use

    CRISPR

    Tags

    2xUGI-BPNLS-P2A-EGFP and BPNLS-TadA-CDd

    Expression

    Mammalian

    Mutation

    TadA-CDd mutations; nSpCas9(D10A/LWKYQS)=D1135L, …

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. delta PE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52380

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted -1620 - -1011 upstream from OCT4 ATG

    Promoter

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. delta DE OCT4-GFP-2A-PURO transgenic reporter

    Plasmid

    #52381

    Purpose

    BAC recombineering generated construct, used as a reporter for OCT4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP-2A-puro
    OCT4 (POU5F1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted -3194 - -1768 upstream from OCT4 ATG

    Promoter

    OCT4

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mouse deltaPE Oct4-GFP transgenic reporter

    Plasmid

    #52382

    Purpose

    BAC recombineering generated construct, used as a reporter for mouse Oct4 expression

    Depositor

    Jacob Hanna

    Article

    Gafni et al Nature. 2013 Dec 12;504(7479):282-6. doi

    Inserts

    GFP pA
    Oct4 (Pou5f1 Mouse)
    PGK-gb2-Neo
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    deleted -300 - -1300bp fragment upstream from Oct…

    Promoter

    Oct4 and PGK+gb2

    Available since

    Sept. 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge