166,034 results

1. IronFistMUT.

   Plasmid

   #243008

   Purpose

   Iron binding deficient IronFist

   Depositor

   Roland Malli

   Article

   Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

   Inserts

   hemerythrin-like-mNeonGreen (FBXL5 Human)
   T2A
   mCherry
   

   Use

   Gateway

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   H15A, H57A, E58A, E61A, H126A, E130A

   Promoter

   human phosphoglycerate kinase (hPGK)

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Iron Insensitive Control Construct 2

   Plasmid

   #243007

   Purpose

   Iron Insensitive Control Construct under the same expression promoter used in IronFist

   Depositor

   Roland Malli

   Article

   Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

   Inserts

   mNeonGreen
   T2A
   mCherry
   

   Use

   Gateway

   Expression

   Mammalian

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. IronFist

   Plasmid

   #243006

   Purpose

   Genetically encoded fluorescent iron reporter

   Depositor

   Roland Malli

   Article

   Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

   Inserts

   Hemerythrin-like (FBXL5 Human)
   T2A
   mCherry
   

   Use

   Gateway

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   human phosphoglycerate kinase (hPGK)

   Available Since

   Sept. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PEA1-Puro

   Plasmid

   #171991

   Purpose

   Delivers all prime editing nickase components in a single, puromycin selectable plasmid

   Depositor

   Paul Thomas

   Article

   Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

   Insert

   CbH-Cas9(H840A)-RT-T2A-Puro, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
   

   Expression

   Mammalian

   Promoter

   CMV for Cas9, U6 for gRNAs

   Available Since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pPBbsr-mKO-MK2

   Plasmid

   #115492

   Purpose

   Expression vector for the p38 activity reporter mKO-MK2 in mammalian cells

   Depositor

   Kazuhiro Aoki

   Article

   Miura et al Cell Rep. 2018 Sep 4;24(10):2658-2668. d

   Insert

   mKO-MK2 (MAPKAPK2 Human)
   

   Tags

   monomeric Kusabira Orange (mKO)

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCMV-PE-Plus

   Plasmid

   #214079

   Purpose

   Mammalian expression of SpCas9 PEmax prime editor with P2A-human P53DD and IRES-human MLH1dn

   Depositor

   Ting Zhou

   Article

   Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

   Insert

   PE-Plus
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pKLV-EF1amCherry-2A-OsTIR1-W

   Plasmid

   #159296

   Purpose

   Lentiviral vector expressing mCherry and OsTIR1 driven by human EF1a promoter for the auxin-inducible degron (AID) system

   Depositor

   Kosuke Yusa

   Article

   Au et al Leukemia. 2021 Apr;35(4):1012-1022. doi:

   Insert

   OsTIR
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   human EF1a promoter

   Available Since

   Sept. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

   Plasmid

   #111568

   Purpose

   Expresses a plasma membrane calcium pump hPMCA2w/b

   Depositor

   Baljit Khakh

   Article

   Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

   Has Service

   AAV5

   Insert

   Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
   

   Use

   AAV

   Tags

   mCherry

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pKLV2-U6gRNA5(Empty)-PGKmCherry2AGFP-W

   Plasmid

   #67981

   Purpose

   Cas9 activity reporter (control) with mCherry and GFP

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Mutation

   Deleted BbsI site within WPRE

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. CMV-SEAP

    Plasmid

    #24595

    Depositor

    Alan Cochrane

    Insert

    SEAP (ALPP Human)
    

    Expression

    Mammalian

    Available Since

    April 23, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pUCKanR-Mu-BsaI

    Plasmid

    #79769

    Purpose

    Transposon-propagation plasmid for Mu-BsaI modified transposon

    Depositor

    David Savage

    Article

    Nadler et al Nat Commun. 2016 Jul 29;7:12266. doi: 10

    Insert

    Mu-BsaI
    

    Available Since

    July 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221_SLC4A7

    Plasmid

    #132204

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC4A7 (SLC4A7 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 28, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

    Plasmid

    #67982

    Purpose

    Cas9 activity reporter with mCherry and GFP

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Mutation

    Deleted BbsI site within WPRE

    Available Since

    Nov. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FIRES-PBCS-CLV3

    Plasmid

    #228697

    Purpose

    Sensor cassette FIRE1, FIRE fluorescent ratiometric sensor

    Depositor

    Carolyn Sangokoya

    Article

    Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

    Insert

    FIRE1 SENSOR
    

    Use

    Synthetic Biology and Unspecified

    Tags

    mClover3

    Expression

    Mammalian

    Promoter

    hpgk

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. FIREC-PBCS-MC2

    Plasmid

    #228698

    Purpose

    Control cassette FIRE1, FIRE fluorescent ratiometric sensor

    Depositor

    Carolyn Sangokoya

    Article

    Sangokoya et al Cell Rep Methods. 2025 Jan 11:100960. do

    Insert

    FIRE1 CONTROL
    

    Use

    Synthetic Biology and Unspecified

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    EF1-alpha

    Available Since

    Jan. 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. phage UbiC tagRFP-T-DDX6

    Plasmid

    #119947

    Purpose

    Lentiviral vector expressing tagRFP-T-DDX6 fusion protein

    Depositor

    Jeffrey Chao

    Article

    Wilbertz et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

    Insert

    DDX6 (DDX6 Human)
    

    Use

    Lentiviral

    Tags

    tagRFP-T

    Expression

    Bacterial and Mammalian

    Promoter

    UbiC

    Available Since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA5/FRT/TO-GAlphasS-RLuc8

    Plasmid

    #140980

    Purpose

    Encodes a G alpha subunit (GNAS2) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociation

    Depositor

    Bryan Roth

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Insert

    GAlphasS-RLuc8
    

    Use

    Luciferase

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NW50

    Plasmid

    #133445

    Purpose

    Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW50
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOSIP-KH-RBS2-dCas9

    Plasmid

    #182740

    Purpose

    Integrative plasmid at the HK022 site in E. coli carrying dCas9 under the control of a Ptet promoter

    Depositor

    David Bikard

    Article

    Rousset et al PLoS Genet. 2018 Nov 7;14(11):e1007749.

    Insert

    dCas9
    

    Use

    CRISPR; Integrative vector

    Promoter

    Ptet

    Available Since

    May 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-TRE-DIO-FLPo

    Plasmid

    #118027

    Purpose

    AAV vector for sparse and bright labeling in a cell-type specific manner

    Depositor

    Minmin Luo

    Article

    Lin et al Nat Methods. 2018 Nov 19. pii: 10.1038/s

    Insert

    TRE-DIO-FLPo
    

    Use

    AAV

    Promoter

    TRE

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge