We narrowed to 1,005 results for: Psp

1. pX458-ECFP

   Plasmid

   #112220

   Purpose

   Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and ECFP

   Depositor

   Yinming Liang

   Article

   Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

   Insert

   ECFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLyGo-Ec-8

   Plasmid

   #163136

   Purpose

   pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P(rhaB)

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. Cas9-GFP_sg_mAMPKa1

   Plasmid

   #79004

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 1.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa1 (Prkaa1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

4. Cas9-GFP_sg_mAMPKa2

   Plasmid

   #79005

   Purpose

   Expresses WT Cas9 and GFP, along with a sgRNA to mouse AMPK alpha 2.

   Depositor

   Reuben Shaw

   Article

   Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

   Insert

   Prkaa2 (Prkaa2 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 22, 2016

   Availability

   Academic Institutions and Nonprofits only

5. TC1332

   Plasmid

   #164874

   Purpose

   CURE-N: C to U RNA Editing, with high efficiency and low off-target effects

   Depositor

   Tian Chi

   Article

   Huang et al EMBO J. 2020 Nov 16;39(22):e104741. doi:

   Insert

   hAPOBEC3A fused to PspCas13b (APOBEC3A )
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   human apobec Y132D

   Promoter

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Cathepsin S

   Plasmid

   #84694

   Purpose

   for expression of Cathepsin S in Leishmania tarantolae

   Depositor

   Hans Brandstetter

   Article

   Freier et al Sci Rep. 2015 Aug 3;5:12707. doi: 10.103

   Insert

   cathepsin S (CTSS Human)
   

   Use

   Expression in leishmania tarantolae

   Tags

   his-tag

   Expression

   Mutation

   Promoter

   unknown

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLPP5

   Plasmid

   #209967

   Purpose

   Contains Level 0 Part: inducible Promoter (PsppQ) with two regulatory genes sppK and sppR for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P_sppQ)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. CCR5_gRNA_pX459

   Plasmid

   #192268

   Purpose

   gRNA expression for CRISPR Genome Editing of CCR5

   Depositor

   Shantanu Chowdhury

   Article

   Sharma et al Cell Rep. 2021 May 18;35(7):109154. doi:

   Insert

   CCR5 guide RNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. HA, EGFP Spastin M87 with exon 4

   Plasmid

   #167542

   Purpose

   For doxycycline inducible mammalian expression of EGFP tagged Spastin M87 with exon 4.

   Depositor

   Tarun Kapoor

   Article

   Cupido et al Nat Chem Biol. 2019 May;15(5):444-452. d

   Insert

   Spastin M87 with exon 4 (SPAST Human)
   

   Use

   Tags

   EGFP and HA tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. px459-RhebL1 sgRNA

    Plasmid

    #133769

    Purpose

    Expresses Cas9 and human RhebL1 sgRNA

    Depositor

    Shawn Ferguson

    Article

    Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

    Insert

    RhebL1 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-Tag/M1 S245X

    Plasmid

    #92366

    Purpose

    expression of M1 S245X spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-194 bp of 5'UTR deleted, S245X

    Promoter

    CMV

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-Tag 3B/WT myc-M87

    Plasmid

    #87722

    Purpose

    Expresses WT myc-M87 spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M87 isoform (deletion of nt 1-490 - 5'UTR an…

    Promoter

    CMV

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA-HEK3-Puro

    Plasmid

    #136282

    Purpose

    Expresses a guide RNA targeting HEK3 site

    Depositor

    Zhaohui Ye

    Article

    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:

    Insert

    sgRNA-HEK3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-Tag/M87 S245X

    Plasmid

    #92368

    Purpose

    expression of M87 S245X spastin in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Mol Biol Cell. 2017 May 11. pii: mbc.E17

    Insert

    spastin (SPAST Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    1-460 bp deleted, S245X

    Promoter

    CMV

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHMWGFPR

    Plasmid

    #64396

    Purpose

    High molecular weight glutenin gene promoter driving GFP expression

    Depositor

    Claudia Vickers

    Article

    Vickers et al Plant Mol Biol. 2006 Sep;62(1-2):195-214

    Insert

    High molecular weight glutenin gene promoter
    

    Use

    Tags

    GFP

    Expression

    Plant

    Mutation

    Promoter

    High molecular weight glutenin gene promoter

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

16. Cdc42 T17N sp64T

    Plasmid

    #16672

    Depositor

    De-Li Shi

    Article

    Djiane et al Development. 2000 Jul . 127(14):3091-100

    Insert

    Cdc42 T17N (CDC42 Human)
    

    Use

    Xenopus expression

    Tags

    Expression

    Mutation

    T17N. Dominant negative.

    Promoter

    Available since

    April 28, 2008

    Availability

    Academic Institutions and Nonprofits only

17. 5xtetO (tet3) Gene Desert

    Plasmid

    #131339

    Purpose

    gRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    Desert-gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. JARID2-KO-gRNA-1

    Plasmid

    #131328

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

19. JARID2-KO-gRNA-2

    Plasmid

    #131327

    Purpose

    gRNA to knockout JARID2. Use with JARID2-KO-gRNA-1

    Depositor

    Danny Reinberg

    Article

    Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

    Insert

    JARID2-KO-gRNA-2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

20. px459-Rheb sgRNA

    Plasmid

    #133768

    Purpose

    Expresses Cas9 and human Rheb sgRNA

    Depositor

    Shawn Ferguson

    Article

    Angarola et al Mol Biol Cell. 2019 Oct 15;30(22):2750-2

    Insert

    Rheb sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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