We narrowed to 1,704 results for: pyogenes Cas9

1. pSLPB2B-ER50-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187948

   Purpose

   ER50 degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   ER50-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLPB2B-SMASh-SpdCas9-tagRFPt-P2A-tagBFP-PGK-Blasticidin

   Plasmid

   #187949

   Purpose

   SMASh degron-tagged dCas9 fused with tagRFPt, P2A site and tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.

   Depositor

   Edda Schulz

   Article

   Noviello et al Nat Commun. 2023 Jun 3;14(1):3225. doi:

   Insert

   SMASh-SpdCas9-tagRFPt-P2A-tagBFP
   

   Use

   CRISPR and Synthetic Biology

   Tags

   GGS linker

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-SpdCas9-VP64

   Plasmid

   #115794

   Purpose

   Expresses SpdCas9-Vp64 fusion protein when packaged into AAV

   Depositor

   Nadav Ahituv

   Article

   Matharu et al Science. 2018 Dec 13. pii: science.aau06

   Insert

   VP64
   

   Use

   AAV

   Tags

   3XFLAG and dCas9-VP64

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Mm.cargo(Cas9)

   Plasmid

   #174865

   Purpose

   Expresses mouse Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by MmPeg10 UTRs
   

   Use

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-Hs.cargo(Cas9)

   Plasmid

   #174864

   Purpose

   Expresses human Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by HsPeg10 UTRs
   

   Use

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDNR-SpCas9-Cdt1

   Plasmid

   #80426

   Purpose

   Used for in vitro transcription of SpCas9-Cdt1 mRNA. Cas9 is fused to Cdt1 peptide and is degraded during S/G2 phases of the cell-cycle.

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Cdt1
   

   Use

   CRISPR

   Tags

   Cdt1

   Expression

   Mutation

   codon optimized for human

   Promoter

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDNR-SpCas9-Gem

   Plasmid

   #80424

   Purpose

   Used for in vitro transcription of SpCas9-Gem mRNA. Cas9 is fused to Geminin peptide and is degraded during G0/G1 phases of the cell-cycle to minimize indels caused by non-homologous end joining

   Depositor

   Sara Howden, Melissa Little

   Article

   Howden et al Stem Cell Reports. 2016 Sep 13;7(3):508-

   Insert

   SpCas9-Gem
   

   Use

   CRISPR

   Tags

   Geminin

   Expression

   Mutation

   codon optimized for human

   Promoter

   T7

   Available since

   Aug. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpCas9(BB)-2A-Puro-V2.0-sgHsXRN1_AH

   Plasmid

   #148859

   Purpose

   Mammalian Expression of HsXRN1-sgRNA

   Depositor

   Elisa Izaurralde

   Insert

   HsXRN1-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6A_AH

   Plasmid

   #148849

   Purpose

   Mammalian Expression of HsTNRC6A-sgRNA1

   Depositor

   Elisa Izaurralde

   Insert

   HsTNRC6A-sgRNA1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpCas9(BB)-2A-Puro-V2.0-sg1HsTNRC6B_AH

    Plasmid

    #148850

    Purpose

    Mammalian Expression of HsTNRC6B-sgRNA1

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6B-sgRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSpCas9(BB)-2A-Puro-V2.0-sg2HsTNRC6A_AH

    Plasmid

    #148852

    Purpose

    Mammalian Expression of HsTNRC6A-sgRNA2

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6A-sgRNA2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSpCas9(BB)-2A-Puro-V2.0-sg3HsTNRC6B_AH

    Plasmid

    #148853

    Purpose

    Mammalian Expression of HsTNRC6B-sgRNA3

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRC6B-sgRNA3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSpCas9(BB)-2A-Puro-V2.0-sgHsPatL_AH

    Plasmid

    #148857

    Purpose

    Mammalian Expression of HsPatL1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsPatL1-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSpCas9(BB)-2A-Puro-V2.0-sgHsTNRC6C_AH

    Plasmid

    #148858

    Purpose

    Mammalian Expression of HsTNRc6C-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsTNRc6C-sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO3_AH

    Plasmid

    #148855

    Purpose

    Mammalian Expression of HsAGO3-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO3-sgRNA (AGO3 S.pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSpCas9(BB)-2A-Puro-V2.0-sg1HsAGO2_AH

    Plasmid

    #148848

    Purpose

    Mammalian Expression of HsAGO2-sgRNA1

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO2-sgRNA1 (AGO2 S.pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSpCas9(BB)-2A-Puro-V2.0-sg2HsAGO2_AH

    Plasmid

    #148851

    Purpose

    Mammalian Expression of HsAGO2-sgRNA2

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO2-sgRNA2 (AGO2 S.pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSpCas9(BB)-2A-Puro-V2.0-sgHsAGO1_AH

    Plasmid

    #148854

    Purpose

    Mammalian Expression of HsAGO1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsAGO1-sgRNA (AGO1 S.pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-2A-Puro-V2.0-sgHsNot1_AH

    Plasmid

    #148856

    Purpose

    Mammalian Expression of HsNot1-sgRNA

    Depositor

    Elisa Izaurralde

    Insert

    HsNot1-sgRNA (CNOT1 S.pyogenes)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX334-U6-DR-BB-DR-Cbh-NLS-hSpCas9n(D10A)-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42333

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9 nickase, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    Available since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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