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Plasmid#52532PurposeDouble-GFP reporter for DNA cleavageDepositorInsertDouble-GFP
UseTagsExpressionInsectMutationPromoterubiquitinAvailable sinceApril 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
PHMcc(delta16 PHM 356s)
Plasmid#25455DepositorInsertPHM (Pam Rat)
UseTagsExpressionMammalianMutationContains the catalytic core of PHM, including aa …PromoterAvailable sinceJune 17, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-I70V/D91G
Plasmid#47644PurposepAC-EsaR with a208g and a272g mutations in esaRDepositorInsertesaR-I70V/D91G
UseSynthetic BiologyTagsExpressionBacterialMutationChanged Isoleucine 70 to Valine and Aspartic acid…PromoterPlacAvailable sinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
SRGAP2C_pEF-DEST51
Plasmid#238497PurposeIVT mRNA of human geneDepositorInsertSRGAP2C (SRGAP2C Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
PDZK1P1_pGCS1
Plasmid#238501PurposeIVT mRNA of human geneDepositorInsertPDZK1P1 (PDZK1P1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
ARHGAP11B_pEF-DEST51
Plasmid#238498PurposeIVT mRNA of human geneDepositorInsertARHGAP11B (ARHGAP11B Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3230
Plasmid#234102PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertFuGFP
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceMay 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisA-NLS-BFP-mEosEM
Plasmid#218934PurposeExpress the nuclear import substrate NLS-BFP-mEosEM.DepositorInsertBFP-mEosEM fusion protein
UseTags6xHis and SV40 nuclear localization sequence (NLS…ExpressionBacterialMutationPromoterT7Available sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3397
Plasmid#234081PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertFuGFP
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3224
Plasmid#234086PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertFuGFP
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceApril 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYI277
Plasmid#228285PurposeConstitutive EGFP expressionDepositorInsertEGFP
UseSynthetic BiologyTagsExpressionYeastMutationPromoterKpGAPDH promoterAvailable sinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKEK3200
Plasmid#234098PurposeCCP expression in diverse gram-negative bacteria, with pre-cloned Golden Gate Compatible (BsaI) restriction sites to facilitate promoter swapping.DepositorInsertfuGFP
UseTagsExpressionBacterialMutationPromoterpJ23100-BsaIAvailable sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNS3_Ptrc::lasR_Plas::mNG
Plasmid#189573PurposeFluorescent reporter for Las activation by 3OC12-HSLDepositorInsertsTranscriptional activator lasR
Monomeric Neon Green
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterlas and trcAvailable sinceDec. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
PTet-F30-Pepper aptamer-UTR1-mTurq2
Plasmid#227645PurposepTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.DepositorInsertF30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD_FLAG-GFP(Y39TAG)-6His
Plasmid#223510PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
UseTags6xHis and FLAGExpressionBacterialMutationY39TAGPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-ATP
Plasmid#225247PurposeBacterial expression of N-terminal His-tagged qmTQ2-ATP, a cyan fluorescent FLIM biosensor for ATP imagingDepositorInsertqmTQ2-ATP
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-glucose
Plasmid#225252PurposeBacterial expression of N-terminal His-tagged qmTQ2-glucose, a cyan fluorescent FLIM biosensor for detecting glucoseDepositorInsertqmTQ2-glucose
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRSETA-qmTQ2-cAMP
Plasmid#225249PurposeBacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMPDepositorInsertqmTQ2-cAMP
UseTags6XHis, T7 tag, XpressTM tagExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV nEF 2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
Plasmid#219653PurposeGeneral mammalian expression of PACmn, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity, with dark VenusDepositorInsert2xLyn-ERex-Venus(Y145W)-bPAC(F198Y)
UseAAVTags2x Lyn11 (GCIKSKGKDS) and ER exit (FCYENE)ExpressionMammalianMutationPromoternEf Addgene # 149296Available sinceMay 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
delCMV-mCitrine
Plasmid#214144PurposeVolume marker / Control sensorDepositorInsertmCitrine
UseTagsExpressionMammalianMutationPromoterdelCMVAvailable sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only