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Showing: 8241 - 8260 of 68612 results

  2. Tn7 integration plasmid

    Plasmid
    #154134
    Purpose
    Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.
    Depositor
    Shimon Bershtein
    Publication
    Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038
    Inserts
    left and right end of Tn7 arm
    Spectinomycin resistant gene
    Expression
    Bacterial
    Promoter
    Spectinomycin resistance gene promoter and no
    Available since
    July 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    154134-plasmid-map-sequence-id-306811

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  4. pCKmatBC

    Plasmid
    #138587
    Purpose
    matBC cassette from R. trifolii controlled by a Plac promoter; pSC101 ori, cat
    Depositor
    Markus Jeschek
    Publication
    Jeschek et al Metab Eng. 2017 Mar;40:33-40. doi: 10.10
    Insert
    bicistronic cassette containing a malonate transporter (matC) and a malonyl-CoA synthetase (matB)
    Use
    Synthetic Biology
    Expression
    Bacterial
    Mutation
    matB contains native GUG start codon
    Promoter
    Plac promoter
    Available since
    March 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    138587-plasmid-map-sequence-id-274478

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  5. pAH-CTX1-rhadCas9

    Plasmid
    #129391
    Purpose
    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.
    Depositor
    Silvia Cardona
    Publication
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Burkholderia cenocepacia codon-optimized dCas9
    Use
    CRISPR
    Expression
    Bacterial
    Mutation
    Entire dCas9 codon optimized for the GC-rich B. c…
    Promoter
    PrhaBAD
    Available since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    129391-plasmid-map-sequence-id-273754

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  7. pDONR221_SV2A

    Plasmid
    #132257
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SV2A (SV2A Human)
    Expression
    Mammalian
    Available since
    Nov. 21, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    132257-plasmid-map-sequence-id-263895

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  8. pDONR221_SLC5A2

    Plasmid
    #132241
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SLC5A2 (SLC5A2 Human)
    Expression
    Mammalian
    Available since
    Nov. 21, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    132241-plasmid-map-sequence-id-263874

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  9. pDONR221_SLC16A1

    Plasmid
    #131934
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors
    Depositor
    RESOLUTE Consortium, Giulio Superti-Furga
    Publication
    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SLC16A1 (SLC16A1 Human)
    Expression
    Mammalian
    Available since
    Oct. 16, 2019
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    131934-plasmid-map-sequence-id-259498

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  14. pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

    Plasmid
    #105127
    Purpose
    Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein
    Depositor
    Kimberley Evason, Didier Stainier
    Publication
    Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d
    Inserts
    Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Promoter
    cryaa (crystallin) and fabp10a
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    105127-plasmid-map-sequence-id-220518

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  15. pAP114

    Plasmid
    #120799
    Purpose
    E. coli-C. difficile shuttle vector for xylose-inducible expression in C. difficile; Pxyl driving expression of red fluorescent protein (mCherryOpt)
    Depositor
    Craig Ellermeier, David S. Weiss
    Publication
    Muh et al J Bacteriol. 2019 Feb 11. pii: JB.00711-
    Insert
    Pxyl::mCherryOpt
    Expression
    Bacterial
    Mutation
    1.4 kb xylR-Pxyl DNA fragment from C. difficile R…
    Promoter
    Pxyl
    Available since
    Feb. 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    120799-plasmid-map-sequence-id-235314

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  17. LLP185 pLVP-dCas9-DNMT3a V2

    Plasmid
    #100936
    Purpose
    dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone
    Depositor
    Ryan Lister
    Publication
    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.
    Insert
    dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
    Use
    CRISPR and Lentiviral
    Tags
    3xHA and 3xTy1
    Expression
    Mammalian
    Mutation
    D10A, H840A for dCas9
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    100936-plasmid-map-sequence-id-206124

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  20. pEGFP-MyosinVa-Ctail

    Plasmid
    #110169
    Purpose
    Dominant negative effect by competing with MyosinVa C-terminal tail interactions
    Depositor
    Jose Esteban
    Publication
    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Myosin Va (Myo5a Mouse)
    Tags
    EGFP and Myc
    Expression
    Mammalian
    Mutation
    amino acids 1444-1853
    Promoter
    CMV
    Available since
    May 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    110169-plasmid-map-sequence-id-210484

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Showing: 8241 - 8260 of 68612 results