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We narrowed to 8,846 results for: FIE

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Showing: 8281 - 8300 of 8846 results

  1. SaCas9v3-Puro

    Plasmid
    #178813
    Purpose
    SaCas9 with 2A-Puro, and a cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    Cbh (Cas9-2A Puro) and U6 (gRNA)
    Available Since
    Aug. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    178813-plasmid-map-sequence-id-354841

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  4. CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA

    Plasmid
    #55201
    Purpose
    Plasmid encoding the Ribozyme/gRNA architecture. This is a modified form of the original plasmid described in the paper (Construct 13). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55201-plasmid-map-sequence-id-240663

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  6. pH-nCas9-PPE-V2

    Plasmid
    #170131
    Purpose
    For plant prime editing in rice plants or monocotyledons protoplasts
    Depositor
    Caixia Gao
    Article
    Lin et al Nat Biotechnol. 2021 Mar 25. pii: 10.103
    Insert
    nCas9(H840A)-M-MLV
    Use
    CRISPR
    Expression
    Plant
    Mutation
    H840A for Cas9; D200N, T306K, W313F, T330P and L…
    Promoter
    maize Ubiquitin-1, OsU3
    Available Since
    May 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    170131-plasmid-map-sequence-id-333607

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  12. GABA(A) receptor subunit g2SE

    Plasmid
    #49170
    Purpose
    pHluorin-tagged GABA A receptor subunit (gamma 2) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.
    Insert
    GABA(A) receptor subunit gamma 2 (Gabrg2 Mouse)
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49170-plasmid-map-sequence-id-165320

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  14. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

    Plasmid
    #55202
    Purpose
    Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Article
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55202-plasmid-map-sequence-id-85589

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  15. pEC2935 (p7INT-P23_mNeongreen~ssrA (LDD variant))

    Plasmid
    #218508
    Purpose
    integrative plasmid enabling constitutive expression of a destabilized mNeongreen reporter in Streptococcus pyogenes, targets the attP site of phage T12 in several S. pyogenes strains via an integrase
    Depositor
    Emmanuelle Charpentier
    Article
    Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1
    Insert
    mNeongreen
    Tags
    modified ssrA degradation tag
    Expression
    Bacterial
    Mutation
    deletion of MCS containing Plac_lacZa, introducti…
    Promoter
    P23
    Available Since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    218508-plasmid-map-sequence-id-438746

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  16. pMS81(rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆)

    Plasmid
    #154840
    Purpose
    Insertion of rpl-28p::mKate2::unc-54 3'UTR into a split hygromycin landing pad. Can be modified to insert a different gene(s) of interest.
    Depositor
    Patrick Phillips
    Article
    Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.
    Insert
    homology arm:rpl-28p::mKate2::unc-54 3'UTR::LoxP::rps-0p::HYGR∆
    Use
    CRISPR and Cre/Lox
    Expression
    Worm
    Mutation
    HYGR∆ encodes aa 1-226
    Available Since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    154840-plasmid-map-sequence-id-307910

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  18. GABA(A) receptor subunit B3SE

    Plasmid
    #49171
    Purpose
    pHluorin-tagged GABA A receptor subunit (beta 3) produces minimal fluorescence during trafficking and a robust fluorescent signal at the cell surface
    Depositor
    Tija Jacob, Stephen Moss
    Article
    Jacob et al J Neurosci. 2005 Nov 9;25(45):10469-78.
    Insert
    GABA(A) receptor subunit beta 3 (Gabrb3 Mouse)
    Tags
    myc, pHGFP (pHluorin), and thrombin cleavage site…
    Expression
    Mammalian
    Mutation
    GTG (V) to TGC (C)
    Promoter
    CMV
    Available Since
    Dec. 10, 2013
    Availability
    Academic Institutions and Nonprofits only
    49171-plasmid-map-sequence-id-72502

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Showing: 8281 - 8300 of 8846 results