We narrowed to 10,068 results for: transfer
-
Plasmid#117622PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.1(pEPOR1CB0011) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)DepositorInsert[35S:Sp-eCas9 1.1(pEPOR1CB0011) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceJune 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0056
Plasmid#117623PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-xCas9 3.7(pEPOR1CB0012) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)DepositorInsert[35S:Sp-xCas9 3.7(pEPOR1CB0012) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0054
Plasmid#117621PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for Sp-eCas9 1.0(pEPOR1CB0010) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)DepositorInsert[35S:Sp-eCas9 1.0(pEPOR1CB0010) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0053
Plasmid#117620PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SpCas9-h(pICSL11023) and sgRNA_Methyltransferase {AT5G10620}(pEPOR1CB0083)DepositorInsert[35S:SpCas9-h(pICSL11023) ] +[AtU6-26:sgRNA]
ExpressionPlantAvailable SinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH861-gst-optec P210T
Plasmid#89595PurposePlasmid for bacterial expression of a His-tagged P210T mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase P210T mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged proline 210 to threoninePromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH846-gst-dissc
Plasmid#96907PurposePlasmid for yeast expression of GST encoded by non-preferred codonsDepositorArticleInsertyeast codon dis-optimised glutathione-S transferase
Tags8xhistidineExpressionYeastPromoterTDH3Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH860-gst-optec A180Q
Plasmid#89594PurposePlasmid for bacterial expression of a His-tagged A180Q mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase A180Q mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged alanine 180 to glutaminePromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH858-gst-optec V156E
Plasmid#89592PurposePlasmid for bacterial expression of a His-tagged V156E mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase V156E mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged valine 156 to glutamic acidPromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTH859-gst-optec Y172M
Plasmid#89593PurposePlasmid for bacterial expression of a His-tagged Y172M mutant of GST encoded by preferred codonsDepositorArticleInsertglutathione-S transferase Y172M mutant, codon-optimised for bacterial expression
Tags8xhistidineExpressionBacterialMutationchanged tyrosine 172 to methioninePromoterT7Available SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
lentiGuide-Puro
Plasmid#52963PurposeExpresses S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter. 3rd generation lentiviral backbone.DepositorInsertsS. pyogenes sgRNA cassette
Puromycin resistance
UseCRISPR and LentiviralExpressionMammalianPromoterEf1-a and hU6Available SinceMay 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3β-FLAG-CBP-HA
Plasmid#32908DepositorAvailable SinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFX-GalT-EGFP
Plasmid#174471PurposeExpress EGFP with golgi apparatus-targeting sequence in mammalian cellsDepositorInsertEGFP with golgi apparatus-targeting sequence
Tagsβ1,4-galactose transferaseExpressionMammalianPromoterCMVAvailable SinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEFBOS_CreIRESpuro
Plasmid#183812PurposeExpresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoterDepositorInsertCre recombinase
UseSynthetic BiologyTagsNLSExpressionMammalianAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
-
pCLucIPZ
Plasmid#53222PurposeConstitutive expression of Cypridina luciferase, puromycin N-acetyl-transferase and shRNAmir of interestDepositorInsertsCypridina luciferase
non-silencing shRNA
UseLentiviralExpressionMammalianAvailable SinceJune 9, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-REPORT(PGK/CMV)-12xHRE
Plasmid#172335Purposehypoxic responsive reporterDepositorInsertsmonomeric nuclear Tomato
d2eGFP
HygR
UseLentiviral and Synthetic BiologyTags3x SV40 NLSExpressionMammalianPromoterPGK/CMVAvailable SinceJune 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-Blast SHMT1
Plasmid#106304PurposeExpress SHMT1DepositorAvailable SinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-dCas9-MQ1(WT)-EGFP
Plasmid#89633PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG and 6*HisExpressionMammalianPromoterCMVAvailable SinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.tgoGAP1
Plasmid#78737PurposeExpression of Ca2+ sensor GAP1 in the trans Golgi apparatusDepositorInserttgoGAP1
TagsN terminal 81 amino acids of human galactosyltran…ExpressionMammalianMutationD117A/D119A/D163A in aequorin moietyPromoterCMVAvailable SinceJuly 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-HygR-mCherry
Plasmid#167189PurposeLentiviral expression of sgRNA with hygromycin resistance gene and mCherryDepositorInsertHygromycin B phosphotransferase and mCherry
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only