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pLminP_Luc2P_RE45

Plasmid

#90383

Purpose

EGR1 - Early growth response factor gene reporter

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

EGR1

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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cfSGFP2-col4a1

Plasmid

#160317

Purpose

cfSGFP2-tagged collagen4a1 for mammalian expression

Depositor

Ikuo Wada

Article

Matsui et al Cell Struct Funct. 2020 Jul 23;45(2):107

Insert

COL4A1 (COL4A1 Human)

Tags

cfSGFP2

Expression

Mammalian

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pJG100

Plasmid

#138966

Purpose

Peft-3::wrmScarlet1-10::unc-54 3’UTR. Expresses wrmScarlet1-10 in somatic tissues of C. elegans

Depositor

Cynthia Kenyon

Article

Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

Insert

wrmScarlet1-10

Expression

Worm

Available Since

July 7, 2020

Availability

Academic Institutions and Nonprofits only

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pSGDlucV3.0

Plasmid

#119760

Purpose

dual luciferase with FMDV StopGo sequences flanking MCS

Depositor

John Atkins

Article

Loughran et al Mol Cell. 2022 Oct 6;82(19):3745-3749.e2

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Promoter

SV40 and T7

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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ZIKV isolate VE_Ganxian2016 NS2B-NS3 protease co-expression of NS2B and NS3 protease domain

Plasmid

#228663

Purpose

Bacterial co-expression of the NS2B and NS3 proteases from Zika virus for crystallization assays in P43 2 2 (PDB:8PN6)

Depositor

ASAP Discovery, Frank von Delft

Insert

Zika virus portion of NS2B protease coexpressed with NS3 protease

Tags

His-GST-TEV

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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PB713B-pCMV-MITD-NeoMini-T2A-tCD19-WPRE

Plasmid

#174610

Purpose

PiggyBack transposon expressing a fusion of a tandem minigene antigens in KRAS, BRAF and CMV fused to an endosomal targeting sequence and translationally linked to human truncated CD19

Depositor

Stanley Riddell

Article

Veatch et al J Clin Invest. 2021 Aug 16;131(16):e1441

Insert

human CD19, antigen minigene

Expression

Mammalian

Available Since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pLumi-LgBiT

Plasmid

#188319

Purpose

Expression of LgBiT via sigma 70 promoter in E. coli

Depositor

Kate Adamala

Article

Sato et al Sci Rep. 2022 Jul 7;12(1):11489. doi: 10

Insert

LgBiT

Use

Luciferase and Synthetic Biology

Expression

Bacterial

Promoter

sigma 70 promoter

Available Since

Sept. 2, 2022

Availability

Academic Institutions and Nonprofits only

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Orai1-jGCaMP8f V203Y

Plasmid

#231631

Purpose

Green fluorescent reporter of Orai1-associated calcium influx

Depositor

Michael Cahalan

Article

Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

Insert

Orai1-jGCaMP8f V203Y (ORAI1 Synthetic, Human)

Tags

jGCaMP8f V203Y

Expression

Mammalian

Promoter

CMV Immediate Early

Available Since

Feb. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti.Syn(0.5).H2B-eGFP.W

Plasmid

#51004

Purpose

Lentivector that expresses H2B-eGFP from neurons-specific synapsin promoter

Depositor

Rusty Lansford

Insert

H2B-EGFP (H2BC11 Synthetic, Human, Jellyfish)

Use

Lentiviral

Tags

histone2B

Expression

Mammalian

Promoter

rat synapsin

Available Since

June 2, 2014

Availability

Academic Institutions and Nonprofits only

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pICE-EGFP-FLAG-PLIN3

Plasmid

#161918

Purpose

Expresses human PLIN3 with a N-terminal GFP-FLAG tag. Confers Puromycin resistance. Inducible in T-REx cells.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

Perilipin-3 (PLIN3 Human)

Tags

GFP-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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R777-E163 Hs.PIK3CA

Plasmid

#70447

Purpose

Gateway ORF clone of human PIK3CA [NM_006218.2] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

PIK3CA (PIK3CA Human)

Use

Gateway entry clone

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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4xCSL-luciferase

Plasmid

#41726

Depositor

Raphael Kopan

Article

Saxena et al J Biol Chem. 2001 Oct 26;276(43):40268-7

Insert

4xCSL binding sites

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains a multimerized high affinity CSL binding…

Available Since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pEB1-T-Sapphire

Plasmid

#103977

Purpose

Plasmid encoding T-Sapphire

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

T-Sapphire

Use

Low copy

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (Q69M, C70V, …

Promoter

proC

Available Since

Dec. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pJL1-mOrange

Plasmid

#102632

Purpose

In vitro expression of mOrange from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al Sci Adv. 2018 Aug 1;4(8):eaat5107. doi:

Insert

mOrange

Expression

Bacterial

Promoter

T7

Available Since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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CCR2-DuET

Plasmid

#213198

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

CCR2 (CCR2 Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-WT

Plasmid

#25361

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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CMVTO-KRAS-G12V

Plasmid

#179654

Purpose

KRAS active mutant (G12V mutation)

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

KRAS G12V

Expression

Mammalian

Mutation

G12V

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST17-TARG1

Plasmid

#172594

Purpose

For purification (from E.coli) of His-tagged TARG1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

TARG1 (OARD1 Human)

Tags

His

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-VSVG

Plasmid

#35616

Depositor

Arthur Nienhuis, Patrick Salmon

Insert

VSV G protein

Expression

Mammalian

Available Since

July 20, 2012

Availability

Academic Institutions and Nonprofits only

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pDARMO_HUWE1_3xFLAG

Plasmid

#187155

Purpose

mammalian cell expression of HUWE1-3xFLAG

Depositor

Eric Fischer

Article

Hunkeler et al Mol Cell. 2021 Sep 2;81(17):3468-3480.e7

Insert

HUWE1 (HUWE1 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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