165,550 results

1. pXL-BacII-CAG-mCherry

   Plasmid

   #170519

   Purpose

   Plasmid containing piggyBac ITRs flanking CAG and mCherry (also see doi: 10.7554/eLife.63907 )

   Depositor

   Joshua Sanes

   Article

   Yamagata et al Bio Protoc. 2021 Aug 5;11(15):e4105. doi

   Insert

   CAG-mCherry
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Feb. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPdR

   Plasmid

   #85083

   Purpose

   Expresses putidaredoxin reductase in E. coli

   Depositor

   Teruyuki Nagamune

   Article

   Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

   Insert

   Putidaredoxin reductase
   

   Tags

   His6 tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAXEF-Rap1-WT

   Plasmid

   #32697

   Depositor

   Vicki Boussiotis

   Article

   Lafuente et al Dev Cell. 2004 Oct;7(4):585-95.

   Insert

   RAP1 (RAP1A Human)
   

   Expression

   Mammalian

   Promoter

   EF promoter

   Available Since

   Nov. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

4. EGFP-Cyclin F

   Plasmid

   #215054

   Purpose

   Expresses EGFP-Cyclin F in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCNF (CCNF Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pZE21-GFPaav

   Plasmid

   #26643

   Depositor

   Michael Elowitz

   Article

   Elowitz et al Nature. 2000 Jan 20. 403(6767):335-8.

   Insert

   pZE21-GFPaav
   

   Tags

   GFPaav

   Expression

   Bacterial

   Mutation

   na

   Available Since

   June 22, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNF02-mLemon

   Plasmid

   #219399

   Purpose

   Constitutive expression of mLemon in E. coli

   Depositor

   Christian Lesterlin

   Article

   Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

   Insert

   mLemon
   

   Expression

   Bacterial

   Promoter

   proDp

   Available Since

   July 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSimpleII-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47867

   Purpose

   This plasmid encode NmCas9 with two NLS and a HA tag

   Depositor

   Erik Sontheimer, James Thomson

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Insert

   NmCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs-IP-mVenus-TOSI

   Plasmid

   #172491

   Purpose

   mVenus-TOSI (TOr-Signal-Indicator) is a fluorescent reporter for mTORC1 signaling (monitoring PDCD4 degradation) which can be introduced to mammalian cells by retrovirus vector.

   Depositor

   David Scadden

   Article

   Oki et al Nat Commun. 2021 Jan 11;12(1):245. doi:

   Insert

   Pdcd4 (Pdcd4 Mouse)
   

   Use

   Retroviral

   Tags

   mVenus

   Expression

   Mammalian

   Promoter

   MLV LTR

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. mCh-Sec61 beta

   Plasmid

   #49155

   Purpose

   expression of Sec61 beta fused to mCherry, used as a general ER marker

   Depositor

   Gia Voeltz

   Article

   Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

   Insert

   Sec61 beta (SEC61B Human)
   

   Tags

   mCherry*

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pATetO 6XHis T4 DNA Ligase

    Plasmid

    #206207

    Purpose

    Inducible expression of T4 DNA ligase

    Depositor

    Andrew Ellington

    Article

    Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

    Insert

    T4 DNA ligase
    

    Expression

    Bacterial

    Available Since

    Dec. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lentiviral-S-tdTomato

    Plasmid

    #112579

    Purpose

    Expressing Shuttle-tdTomato, or NLS-NES-tagged tdTomato in a Lentiviral vector

    Depositor

    Jeffrey Rothstein

    Article

    Zhang et al Nature. 2015 Sep 3;525(7567):56-61. doi:

    Insert

    NLS-tdTomato-NES
    

    Use

    Lentiviral

    Tags

    nuclear export sequence and nuclear localization …

    Expression

    Mammalian

    Available Since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL2787

    Plasmid

    #15460

    Depositor

    Chia Lee

    Article

    Luong et al J Microbiol Methods. 2007 Jul . 70(1):18

    Insert

    phage phi11 int gene
    

    Expression

    Bacterial

    Available Since

    Oct. 9, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. 809 pcDNA3 GFP PTEN

    Plasmid

    #10759

    Depositor

    William Sellers

    Article

    Vazquez et al J Biol Chem. 2001 Dec 28. 276(52):48627-

    Insert

    PTEN (PTEN Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Feb. 13, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nup133_mEGFP(-8a)

    Plasmid

    #163417

    Purpose

    Mammalian expression of Nup133 fused at its carboxyl-terminus to mEGFP with no linker and a total net fusion of (-8 amino acids)

    Depositor

    Sanford Simon

    Insert

    Nup133 (NUP133 Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCRISPR-PsppA-mCherry

    Plasmid

    #225428

    Purpose

    Plasmid encodes SgRNA, SpCas9 and homologous arms for homologous recombination of mCherry

    Depositor

    Geir Mathiesen

    Article

    Wiull et al Microbiol Spectr. 2025 Feb 4;13(2):e0202

    Insert

    mCherry, Cas9, SgRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    PsppA upstream of mCherry and Cas9, P3 upstream o…

    Available Since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFRT/TO/HIS/FLAG/HA-PRDX1

    Plasmid

    #38086

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PRDX1 peroxiredoxin 1 (PRDX1 Human)
    

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPdX

    Plasmid

    #85084

    Purpose

    Expresses putidaredoxin in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

    Insert

    Putidaredoxin
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    changed Cysteine 73 to Serine and Cysteine 85 to …

    Promoter

    T7 promoter

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV FLEX Synaptophysin GFP

    Plasmid

    #137188

    Purpose

    Cre-dependent expression of synaptophysin-GFP

    Depositor

    Matthew Nolan

    Article

    Surmeli et al Neuron. 2016 Nov 23;92(4):929. doi: 10.1

    Insert

    Synaptophysin GFP (Syp Mouse)
    

    Use

    AAV

    Available Since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. Salsa6f

    Plasmid

    #140188

    Purpose

    Salsa6f is a fluorescent ratiometric genetically encoded calcium indicator, (tdTomato linked to GCaMP6f by a V5 epitope tag)

    Depositor

    Michael Cahalan

    Article

    Dong et al eLife. 2017 Dec 14;6. pii: 32417. doi: 1

    Insert

    Salsa6f
    

    Tags

    tdTomato-v5-GCaMP6f

    Expression

    Mammalian

    Promoter

    CMV Immediate Early

    Available Since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-T7-SpRY-HF1-P2A-EGFP (RTW5008)

    Plasmid

    #139997

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized SpRY-HF1(SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized SpCas9 variant named SpRY-HF1 with BPNLS-3xFLAG-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…

    Promoter

    CMV and T7

    Available Since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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