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pBacT7-S1T3D

Plasmid

#33282

Depositor

Terence Dermody

Article

Kobayashi et al Cell Host Microbe. 2007 Apr 19;1(2):147-

Insert

S1 (S1 Orthoreovirus T3D)

Expression

Mammalian

Promoter

T7

Available Since

Dec. 5, 2011

Availability

Academic Institutions and Nonprofits only

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ZP27 Bmp2b

Plasmid

#16915

Depositor

Randall Moon

Insert

BMP2b (bmp2b Zebrafish)

Expression

Bacterial

Available Since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
HA-HIF2α P405A/P531A Δ820-870-pBabe-Puro

Plasmid

#25949

Depositor

William Kaelin

Article

Yan et al Mol Cell Biol. 2007 Mar . 27(6):2092-102

Insert

Hypoxia inducible factor 2 alpha (EPAS1 Human)

Use

Retroviral

Tags

HA-tag

Expression

Mammalian

Mutation

cDNA changed amino acids: Proline 405 to Alanin…

Available Since

Oct. 4, 2010

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv

Plasmid

#217437

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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pInducer20_EGFP

Plasmid

#228878

Purpose

Tet-inducible lentiviral vector for expression of optimized GFP

Depositor

Ramiro Iglesias-Bartolome

Article

Branch et al bioRxiv [Preprint]. 2024 Oct 3:2024.10.0

Insert

EGFP

Use

Lentiviral

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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plenti.CamKII.(cyto).iATPSnFR2.A95A.A119L.HaloTag

Plasmid

#209724

Purpose

Expresses cytosolic low affinity ratiometric ATP sensor

Depositor

Timothy Ryan

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

(cyto).iATPSnFR2.A95A.A119L.HaloTag

Use

Lentiviral

Expression

Mammalian

Promoter

CamKII

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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SSB-wt

Plasmid

#197260

Purpose

wild type E. coli SSB in pET21 expresses in BL21 under T7 control with lactose-amp resistance

Depositor

Timothy Lohman

Article

Antony et al J Mol Biol. 2013 Nov 29;425(23):4802-19.

Insert

wt E. coli SSB

Tags

none

Expression

Bacterial

Promoter

T7

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Foff DREADD Gq-mCherry

Plasmid

#183533

Purpose

INTRSECT Chemogenetics - expresses DREADD Gq-mCherry in cells expressing Cre but not Flp

Depositor

Karl Deisseroth

Insert

DREADD Gq-mCherry

Use

AAV

Mutation

None

Promoter

nEF

Available Since

May 18, 2022

Availability

Academic Institutions and Nonprofits only

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pDY0310 TP901-1 Integrase

Plasmid

#179110

Purpose

TP901-1 Integrase expression vector

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Article

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Insert

TP901-1 Integrase

Use

CRISPR

Expression

Mammalian

Available Since

Feb. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221_MPC2

Plasmid

#131930

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MPC2 (MPC2 Human)

Expression

Mammalian

Available Since

Oct. 16, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_MFSD8

Plasmid

#131882

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

MFSD8 (MFSD8 Human)

Expression

Mammalian

Available Since

Oct. 9, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-beta-arrestin2-HA-TEV219

Plasmid

#104845

Purpose

b2AR-SPARK protease component

Depositor

Alice Ting

Article

Kim et al Elife. 2017 Nov 30;6. doi: 10.7554/eLife

Insert

beta-arrestin2-HA-TEV219

Use

AAV

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pHA-N1

Plasmid

#86049

Purpose

A mammalian expression vector with a HA-tag at C-terminus.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Type

Empty backbone

Tags

HA tag

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2017

Availability

Academic Institutions and Nonprofits only

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pX33

Plasmid

#89632

Purpose

Allelic change mutagenesis

Depositor

Greg Cook

Article

Gebhard et al Microbiology. 2006 Nov;152(Pt 11):3453-6

Insert

xylE

Use

Allelic change mutagenesis

Promoter

Pmyco

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pGrDL_SPb

Plasmid

#83205

Purpose

receives miRNA target sequences for testing using the dual luciferase assay system

Depositor

Peer Schenk

Article

Moyle et al Front Plant Sci. 2017 Sep 20;8:1631. doi

Insert

Tomato ACTIN promoter

Expression

Plant

Promoter

Tomato ACTIN

Available Since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pGR-BBa_B0010

Plasmid

#46003

Depositor

Christopher Voigt

Article

Chen et al Nat Methods. 2013 Jun 2. doi: 10.1038/nm

Insert

BBa_B0010 Terminator

Use

Synthetic Biology

Expression

Bacterial

Promoter

PBAD

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pDae029 (eMutaT7[TadA-8e])

Plasmid

#187620

Purpose

Expresses eMutaT7[TadA-8e] for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Insert

TadA-8e-T7RNAP

Expression

Bacterial

Available Since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGGS-SspB-mScarlet-I

Plasmid

#178521

Purpose

Expresses SspB-mScarlet-I in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

SspB-mScarlet-I

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-GCaMP6s

Plasmid

#166606

Purpose

Encodes Cre-dependent GCaMP6s under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

GCaMP6s

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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mCRISPRed pRSETB

Plasmid

#159454

Purpose

Expresses the pH-resistant long Stokes shift red fluorescent protein mCRISPRed

Depositor

Oliver Griesbeck

Article

Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

Insert

mCRISPRed

Tags

10 x His-Tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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