59,428 results

1. pSN-A112C-GpA

   Plasmid

   #191238

   Purpose

   Expresses nuclease A-H124L from Staphylococcus aureus, with an A112C mutation, fused through a flexible linker to the transmembrane domain of GpA and a His-tag, for fluorescent studies.

   Depositor

   Alessandro Senes

   Article

   Diaz Vazquez et al Biophys J. 2023 Jan 3;122(1):143-155. do

   Insert

   nuc (nuclease A), GYPA (GPA) (E(transmembrane)R)
   

   Tags

   nuclease A from S. aureus fused to the TM domain …

   Expression

   Bacterial

   Mutation

   Changed Alanine 112 to Cysteine

   Promoter

   T7 promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLQ14566

   Plasmid

   #239272

   Purpose

   Expresses TRF1-PYL1 for recruiting RNA to the telomere via CRISPR-TO

   Depositor

   Stanley Qi

   Article

   Han et al Nature. 2025 Jul;643(8070):241-251. doi:

   Insert

   TRF1-BFP-PYL1 (TERF1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ14806

   Plasmid

   #239273

   Purpose

   Expresses TRF1-PYL1 for recruiting RNA to the nuclear stress body via CRISPR-TO

   Depositor

   Stanley Qi

   Article

   Han et al Nature. 2025 Jul;643(8070):241-251. doi:

   Insert

   HSF1-BFP-PYL1 (HSF1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ10539

   Plasmid

   #239270

   Purpose

   Expresses PYL1-DDX6 for recruiting RNA to the p-body via CRISPR-TO

   Depositor

   Stanley Qi

   Article

   Han et al Nature. 2025 Jul;643(8070):241-251. doi:

   Insert

   PYL1-BFP-DDX6 (DDX6 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   SFFV

   Available Since

   Sept. 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFUW-TetO-IKZF2

   Plasmid

   #207216

   Purpose

   doxycycline-inducible expression of Human IKZF2 in mammalian cells

   Depositor

   Filipe Pereira

   Article

   Henriques-Oliveira et al Immunity. 58, 1-20. October 2025.

   Insert

   pFUW-TetO-IKZF2
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET21a asyn(Δ41-54/Δ103-130)

   Plasmid

   #242236

   Purpose

   Expresses alpha synuclein (missing exon 3 and 5, residues 41-54 and 103-130) in bacteria

   Depositor

   Jennifer Lee

   Article

   SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

   Insert

   asyn(Δ41-54/Δ103-130)
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   deleted amino acids 41-54 and 103-130

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET21a asyn(Δ103-130)

   Plasmid

   #242235

   Purpose

   Expresses alpha synuclein (missing exon 5, residues 103-130) in bacteria

   Depositor

   Jennifer Lee

   Article

   SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

   Insert

   asyn(Δ103-130)
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   deletion of residues 103-130

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a asyn(Δ41-54)

   Plasmid

   #242234

   Purpose

   Expresses alpha synuclein (missing exon 3, residues 41-54) in bacteria

   Depositor

   Jennifer Lee

   Article

   SanGiovanni et al Protein Sci. 2025 Jul;34(7):e70195. doi:

   Insert

   asyn(Δ41-54)
   

   Tags

   none

   Expression

   Bacterial

   Mutation

   deleted amino acids 41-54

   Available Since

   Aug. 5, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5_mEGFP-NUP98-DDX10-KS

   Plasmid

   #237644

   Purpose

   For overexpression of mEGFP-NUP98-DDX10-KS

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   NUP98-DDX10-KS (NUP98, DDX10 Human)
   

   Tags

   mEGFP

   Expression

   Mammalian

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAGGS-MTS-TFAM-V5

    Plasmid

    #200813

    Purpose

    expresses MTS-TFAM in mammalian cells

    Depositor

    Hung-Chun Chang

    Insert

    Transcription Factor A, Mitochondrial (TFAM Human)
    

    Tags

    V5

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pPB-cT3G-cERP2-RARG

    Plasmid

    #222570

    Purpose

    PiggyBac transposon plasmid for doxycycline inducible expression of RARG

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2024.05.31.596483

    Insert

    RARG (RARG Human)
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pmiRFP670-C1-Nir1-LNS2

    Plasmid

    #220085

    Purpose

    high affinity PA biosensor with far-red fluorophore

    Depositor

    Gerry Hammond

    Article

    Weckerly et al J Cell Biol. 2025 Nov 3;224(11):e2024051

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Tags

    iRFP670-GGSGGM

    Expression

    Mammalian

    Mutation

    amino acids 613-897

    Promoter

    CMV

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. SGLT2(GFP)-MAP17(nb)

    Plasmid

    #216211

    Purpose

    for expression of human GFP-tagged SGLT2-MAP17 nanobody complex in BacMam system

    Depositor

    Lei Chen

    Article

    Cui et al Nat Commun. 2023 May 22;14(1):2920. doi:

    Insert

    SGLT2 GFP fusion and MAP17 nanobody fusion (PDZK1IP1, SLC5A2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1-MS2-HA-HsRoquin2_AE

    Plasmid

    #148614

    Purpose

    Mammalian Expression of HsRoquin2

    Depositor

    Elisa Izaurralde

    Article

    Sgromo et al Nat Commun. 2017 Feb 6;8:14307. doi: 10.

    Insert

    HsRoquin2 (RC3H2 Human)
    

    Expression

    Mammalian

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pDONR221-SLC29A2_STOP

    Plasmid

    #161305

    Purpose

    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

    Depositor

    RESOLUTE Consortium, Giulio Superti-Furga

    Article

    Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

    Insert

    SLC29A2 (SLC29A2 Human)
    

    Expression

    Mammalian

    Available Since

    Sept. 15, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. AAVS1 intron 1 gRNA as2

    Plasmid

    #132394

    Purpose

    Targets AAVS1 intron 1, gRNA: AGAACCAGAGCCACATTAAC, MLM3636 backbone

    Depositor

    Gerold Schmitt-Ulms

    Article

    Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

    Insert

    gRNA targeting AAVS1 intron 1 (PPP1R12C Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pIG-830_HA-GD2-28z_CAR_tNGFR_Retroviral

    Plasmid

    #207507

    Purpose

    This plasmid can be used to generate retrovirus.

    Depositor

    Alexander Marson

    Article

    Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

    Insert

    tNGFR, HA-GD2-28z_CAR (NGFR Human)
    

    Use

    Retroviral

    Available Since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-N-Myc_WT USP7

    Plasmid

    #131242

    Purpose

    Mammalian expression of N-terminally Myc-tagged USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV enhancer + CMV promoter

    Available Since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBa-LSS-GFP-LDLR wt

    Plasmid

    #98184

    Purpose

    Low Density Lipoprotein Receptor N-terminally tagged with Green Fluorescent Protein. LSS= LDLR signal sequence, which is cleaved leaving the GFP attached to the mature LDLR protien.

    Depositor

    Marvin Bentley, Gary Banker

    Article

    Jenkins et al J Cell Biol. 2012 Aug 20;198(4):749-61.

    Insert

    LDLR (LDLR Human)
    

    Tags

    GFP and LDLR signal sequence, N-term of GFP so GF…

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Chicken Beta Actin

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. AP1muA-SNAP-V5-polyA-Puro HR

    Plasmid

    #229680

    Purpose

    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.

    Depositor

    Francesca Bottanelli

    Article

    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/

    Insert

    genomic sequence AP1M1 locus (AP1M1 Human)
    

    Use

    CRISPR

    Tags

    SNAP, V5

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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