We narrowed to 1,322 results for: pET28a

1. pET28-p53 (1-73) (72P)

   Plasmid

   #62083

   Purpose

   Expression of human p53 (1-73) (72P) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   HIs

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; 72P

   Promoter

   T7

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

2. HOXB1 (human) HIS-tag pET

   Plasmid

   #8520

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXB1 (HOXB1 Human)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   PCR from cDNA clone

   Promoter

   Available Since

   July 6, 2006

   Availability

   Academic Institutions and Nonprofits only

3. p28atDarpp

   Plasmid

   #108318

   Purpose

   Expresses tDarpp in E.coli

   Depositor

   Jamil Momand

   Article

   Momand et al FEBS Open Bio. 2017 Aug 29;7(9):1328-133

   Insert

   tDarpp (PPP1R1B Human)
   

   Use

   Tags

   6 Histadine

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   July 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET28-p53 (1-73) (72R)

   Plasmid

   #62082

   Purpose

   Expression of human p53 (1-73) (72R) in e. coli

   Depositor

   Gary Daughdrill

   Article

   Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

   Insert

   p53 (TP53 Human)
   

   Use

   Tags

   HIs

   Expression

   Bacterial

   Mutation

   contains TAD residues 1-73; 72R

   Promoter

   T7

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

5. 4E2H.210_LUMABS

   Plasmid

   #155209

   Purpose

   Expresses 4E2H.210_LUMABS sensor in E.coli

   Depositor

   Bruno Correia

   Article

   Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

   Insert

   4E2H.210_LUMABS
   

   Use

   Tags

   6x His Tag

   Expression

   Mutation

   Promoter

   Available Since

   Aug. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. HOXD4 (murine) HIS-tag pET

   Plasmid

   #8548

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXD4 (Hoxd4 Mouse)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   makes protein by Western and by in vitro transcri…

   Promoter

   Available Since

   May 30, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pET-28a-His6-SUMO-RABIF

   Plasmid

   #135556

   Purpose

   Express human RABIF in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   RABIF (RABIF Human)
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available Since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pJK597

   Plasmid

   #71121

   Purpose

   Produces E coli formyl-CoA:oxalate CoA-transferase (H6YfdW) in E. coli

   Depositor

   T. J. Kappock

   Article

   Mullins et al Protein Sci. 2012 May;21(5):686-96. doi:

   Insert

   formyl-CoA:oxalate CoA-transferase
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p47 PX(1-121)/p40phox(136-339)

   Plasmid

   #19018

   Depositor

   Michael Yaffe

   Article

   Bissonnette et al J Biol Chem. 2008 Jan 25. 283(4):2108-19

   Insert

   p40phox/p47phox (NCF4, NCF1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   insert containing amino acids 1-121 of p47phox fu…

   Promoter

   Available Since

   Aug. 25, 2008

   Availability

   Academic Institutions and Nonprofits only

10. hSTX4(1-271,272C)

    Plasmid

    #206031

    Purpose

    Bacterial expression of human Syntaxin 4 without transmembrane helix and a C-terminal cysteine

    Depositor

    Geert van den Bogaart

    Article

    Chen et al J Cell Sci. 2025 Sep 22:jcs.264139. doi:

    Insert

    Syntaxin-4 (STX4 Human)
    

    Use

    Tags

    his-tag (removable with thrombin)

    Expression

    Bacterial

    Mutation

    deleted amino acids 272-297 (encoding the transme…

    Promoter

    T7

    Available Since

    Oct. 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. His-Thrombin-Munc13-1 C1-C2B-MUN-C2C R1598E F1658E (delta1408-1452 delta1533-1551)

    Plasmid

    #243822

    Purpose

    Munc13-1 protein purification

    Depositor

    Jose Rizo-Rey

    Article

    Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

    Insert

    Munc13-1 (Unc13a Rat)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    delta1408-1452 delta1533-1551 R1598E F1658E

    Promoter

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-28a(+)-6xHis-TEV-ORF9c

    Plasmid

    #201023

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    open reading frame 9c
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available Since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Human_TFAM_HMGA_linker_pET28

    Plasmid

    #167327

    Purpose

    Expresses TFAM HMGA+linker mutant in bacterial cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Truncation mutant containing HMGA and linker doma…

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Human_TFAM_HMGB_ctail_pET28

    Plasmid

    #167330

    Purpose

    Expresses TFAM HMGB+C-tatil mutant in bacterial cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Truncation mutant containing HMGB and C-tail doma…

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Human_TFAM_dC_pET28

    Plasmid

    #167328

    Purpose

    Expresses TFAM dC-tail mutant in bacterial cells

    Depositor

    Tom Misteli

    Article

    Feric et al EMBO J. 2021 Mar 15;40(6):e107165. doi:

    Insert

    TFAM (TFAM Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Truncation mutant containing HMGA, linker and HMG…

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. 4E1H.95_LUMABS

    Plasmid

    #155208

    Purpose

    Expresses 4E1H.95_LUMABS sensor in E.coli

    Depositor

    Bruno Correia

    Article

    Yang et al Nat Chem Biol. 2021 Apr;17(4):492-500. d

    Insert

    4E1H.95_LUMABS
    

    Use

    Tags

    6x His Tag

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. His6-pAG-RTase

    Plasmid

    #214932

    Purpose

    Express fusion proteins of Protein A/G and MMLV reverse transcriptase (25-497) in bacterial systems

    Depositor

    Chuan He

    Article

    Xiao et al Nat Methods. 2024 Jan 10. doi: 10.1038/s

    Insert

    fusion protein of protein A/G and MMLV reverse transcriptase (25-497)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    MMLV RTase (H8Y, D200N, T306K, W313F, T330P, D524…

    Promoter

    T7 promoter

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PARP1 FL

    Plasmid

    #169815

    Purpose

    Expresses codon optimised full length His tagged PARP1 in bacterial cells

    Depositor

    David Neuhaus

    Article

    Ogden et al Nucleic Acids Res. 2021 Feb 26;49(4):226

    Insert

    Poly[ADP-ribose] polymerase 1 (PARP1 Codon optimised, Synthetic, Human)
    

    Use

    Tags

    MKHHHHHHMKQ

    Expression

    Bacterial

    Mutation

    Valine 762 mutated to an alanine

    Promoter

    T7 promoter

    Available Since

    Aug. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET28-MBP-super TEV protease

    Plasmid

    #171782

    Purpose

    Expresses Maltose-binding protein-super TEV protease in bacterial cytoplasm. This protease version performs well in the absence of added reducing agent.

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    MBP-sTEV
    

    Use

    Tags

    Arg6 tag and Internal His6 tag

    Expression

    Bacterial

    Mutation

    No internal TEV cleavage site between the MBP and…

    Promoter

    T7

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28:LanIII

    Plasmid

    #225075

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanIII (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIII)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Dec. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28:LanIII-43

    Plasmid

    #225077

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanIII-43 (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanIII-43)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET28:LanII

    Plasmid

    #225066

    Purpose

    Expresses precursor peptide and lanthipeptide synthetase from LanII (FAST-RiPPs) in E. coli

    Depositor

    Wilfred van der Donk

    Article

    Ayikpoe et al Nat Commun. 2022 Oct 17;13(1):6135. doi:

    Insert

    LanA (LanII)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    WT

    Promoter

    T7

    Available Since

    Oct. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. proISG15 (2-165)

    Plasmid

    #110762

    Purpose

    Bacterial expression of proISG15

    Depositor

    David Komander

    Article

    Swatek et al Proc Natl Acad Sci U S A. 2018 Mar 6;115

    Insert

    ISG15 (ISG15 Human)
    

    Use

    Tags

    N-His6-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRD421

    Plasmid

    #163112

    Purpose

    Production of His6_TEV-cleavable-linker_mEos3.2_H-NSdbd in BL21 (DE3) pLysS

    Depositor

    Remus Dame

    Article

    Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

    Insert

    His-tag_TEV-cleavable-linker_mEos3.2_H-NSdbd
    

    Use

    Tags

    His-tag with TEV-cleavable linker

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Brg1 Del4 pET28C

    Plasmid

    #122118

    Purpose

    Expression Brg1 amino acids 1446-1647 in bacteria

    Depositor

    Paul Herring

    Article

    Zhou et al Arterioscler Thromb Vasc Biol. 2009 Jun;

    Insert

    Brg1 (SMARCA4 Human)
    

    Use

    Tags

    His-T7

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHelA12

    Plasmid

    #176493

    Purpose

    Expresses the N-terminal His6-tagged SARS-CoV helicase in E coli.

    Depositor

    Xiaodong Cheng, Jian-Dong Huang

    Article

    Tanner et al J Biol Chem. 2003 Oct 10;278(41):39578-8

    Insert

    SARS-CoV NSP13 (ORF1ab SARS-CoV)
    

    Use

    Tags

    His6 and T7

    Expression

    Bacterial

    Mutation

    V570I

    Promoter

    T7

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HOXB3 (human) HIS-tag pET

    Plasmid

    #8524

    Depositor

    Corey Largman

    Article

    Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

    Insert

    HOXB3 (HOXB3 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains 5 additional amino acids before ATG from…

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

28. C-His-GvpC-Calpain

    Plasmid

    #153295

    Purpose

    Expresses calpain-sensitive variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Calpain-sensitive Gas vesicle protein C (his-tagged)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    1) Contains an extra glycine after the methionine…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. N-His-GvpC-ssrA

    Plasmid

    #153297

    Purpose

    Expresses ClpXP-degradable variant of Anabaena flos-aquae GvpC in E.coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C (with N-terminal his-tag and C-terminal ssrA tag)
    

    Use

    Tags

    His-tag and ssrA tag

    Expression

    Bacterial

    Mutation

    1) N-terminal 6xHis tag flanked by 1x glycine res…

    Promoter

    T7

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. HOXB4 (human) HIS-tag pET new RBS

    Plasmid

    #8525

    Depositor

    Corey Largman

    Article

    Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

    Insert

    HOXB4 (HOXB4 Human)
    

    Use

    Tags

    His and T7

    Expression

    Bacterial

    Mutation

    Contains improved Ribosomal binding site; makes …

    Promoter

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only