We narrowed to 9,441 results for: pho

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pDEST26 Ago2 S387E

Plasmid

#59906

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387E mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387E, phospho-dominant

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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pEF.DEST51-PTPN11(D425A,C459S)-Clover

Plasmid

#215526

Purpose

Expression vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Tags

Clover

Expression

Mammalian

Mutation

D425A, C459S (Phosphatase inactive)

Promoter

EF1a

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pENTR2b-PTPN11(D425A,C459S)-Clover

Plasmid

#215520

Purpose

Gateway entry vector with a clover-tagged phosphatase-inactive Shp2 mutant (D425A,C459S)

Depositor

Johanna Ivaska

Article

Conway et al Nat Cell Biol. 2025 May 26. doi: 10.1038

Insert

PTPN11 (PTPN11 Human)

Use

Gateway entry vector

Tags

Clover

Mutation

D425A, C459S (Phosphatase inactive)

Promoter

none

Available Since

Sept. 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1 mTagBFP2 rat PGK1 shRNA

Plasmid

#222869

Purpose

Expresses mTagBFP2 along with an shRNA against rat PGK1

Depositor

Timothy Ryan

Article

Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

Insert

PGK1 shRNA (Pgk1, Pgk1 Mouse, Rat)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1 PA-PLA1 (EGFP-PA-PLA1)

Plasmid

#162880

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with EGFP

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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flag-hKLF6-4A (1010)

Plasmid

#49489

Purpose

expresses human Flag tagged KLF6 with 4A mutation

Depositor

Scott Friedman

Article

Lang et al Oncogene. 2012 Oct 22. doi: 10.1038/onc.

Insert

KFL6-4A (KLF6 Human)

Tags

Flag

Expression

Mammalian

Mutation

three serines and one tyrosine mutated to alanin…

Promoter

CMV

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only
pDEST26 Ago2 S387A

Plasmid

#59905

Purpose

Expresses 6xHis-Ago2 (N-termi tag) S387A mutant in mammalian cells

Depositor

Carl Novina

Article

Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

Insert

Argonaute 2 (AGO2 Human)

Tags

6xHis (N-termi tag)

Expression

Mammalian

Mutation

S387A, phospho-null alanine

Promoter

CMV

Available Since

May 26, 2015

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S12TAG

Plasmid

#107155

Purpose

MBP-rPKL construct for production and purification of rat PKL pS12

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 12 to TAG for phosphoserine insertion

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-rPKL S113TAG

Plasmid

#107157

Purpose

MBP-rPKL S113TAG for production and purification of rat PKL pS113

Depositor

Jesse Rinehart

Article

Gassaway et al Cell Rep. 2019 Dec 10;29(11):3394-3404.e

Insert

MBP-tagged rat pyruvate kinase liver isoform (Pklr Rat)

Tags

C-term 6-his and N-term MBP

Expression

Bacterial

Mutation

Serine 113 changed to TAG for phosphoserine inser…

Promoter

PBAD

Available Since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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RMCE ABCDE>ala DNA-PKcs

Plasmid

#233914

Purpose

Low copy number provides expression of human DNA-PKcs with the 6 ABCDE phosphorylation sites substituted to alanine.

Depositor

Katheryn Meek

Article

Buehl et al Mol Cell. 2023 Mar 2;83(5):698-714.e4. d

Insert

human DNA-PKcs ABCDE phosphorylation site mutant (PRKDC Human)

Expression

Mammalian

Mutation

phosphorylation sites 2609, 2612, 2620, 2624, 263…

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pCDFDuet-1 MKK4(EE)-MKK7a1(EE)

Plasmid

#47580

Purpose

Bacterial expression plasmid for co-expression of constitutively active MKK4 and MKK7a1

Depositor

Kevin Janes

Article

Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

Inserts

MKK4-EE (MAP2K4 Human)
MKK7a1-EE (Map2k7 Mouse)

Expression

Bacterial

Mutation

Lacks the first 36 residues of the RefSeq protein…

Available Since

Sept. 19, 2013

Availability

Academic Institutions and Nonprofits only
pmEos2-C1 PA-PLA1 (mEos2-PA-PLA1)

Plasmid

#162878

Purpose

Expression in mammalian cells of Phosphatidic Acid preferring Phospholipase A1 tagged with mEos2 to perform sptPALM

Depositor

Frederic Meunier

Article

Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p

Insert

Phosphatidic acid-preferring phospholipase A1 (PA-PLA1) (DDHD1 Human)

Tags

mEos2

Expression

Mammalian

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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MSCV-IRES_Tomato MEF2C S222D

Plasmid

#107231

Purpose

hMEF2C phosphomimetic obtained from Quikchange Lightning Mutagenesis

Depositor

Alex Kentsis

Article

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Retroviral

Expression

Mammalian

Mutation

S222D

Available Since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-TET1-S-WT

Plasmid

#232942

Purpose

Expresses short isoform of TET1 in mammalian cells in its wild-type form

Depositor

Jianjun Chen

Article

Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

Insert

TET1-S-WT (TET1 Human)

Use

Lentiviral

Available Since

May 19, 2025

Availability

Academic Institutions and Nonprofits only

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His-SUMO-Rab10 Q68L

Plasmid

#236720

Purpose

Expresses Rab10 Q68L with His tag and SUMO cleavage sequence

Depositor

Suzanne Pfeffer

Article

Claire Y. et al bioRxiv, 2025

Insert

Rab10 Q68L (RAB10 Human)

Tags

6xHis-SUMO

Expression

Bacterial

Mutation

Q68L

Promoter

T7

Available Since

May 7, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-3_Flag-TET1-S-MUT

Plasmid

#232943

Purpose

Expresses mutant form of the short isoform of TET1 in mammalian cells

Depositor

Jianjun Chen

Article

Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

Insert

TET1-S-MUT (TET1 Human)

Use

Lentiviral

Mutation

H1652Y and D1654A

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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pCDH-no-Flag-TET1-S-WT

Plasmid

#232941

Purpose

Expresses short isoform of TET1 in mammalian cells in its wild-type form

Depositor

Jianjun Chen

Article

Chen et al Sci Transl Med. 2023 Mar 29;15(689):eabq

Insert

TET1-S-WT (TET1 Human)

Use

Lentiviral

Available Since

March 21, 2025

Availability

Academic Institutions and Nonprofits only

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AIMTOR A757

Plasmid

#140829

Purpose

AIMTOR BRET Biosensor containing a mutated non-phosphorylable ULK1 peptide to use as a control constuct in parrallel with AIMTOR T757

Depositor

Anne Bonnieu

Article

Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1

Insert

AIMTOR A757 (ULK1 Human)

Tags

NES Nuclear export signal

Expression

Mammalian

Mutation

The insert comprises only a small sequence of hum…

Available Since

July 10, 2020

Availability

Academic Institutions and Nonprofits only

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pRSETa mEos2

Plasmid

#20341

Purpose

bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

Depositor

Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

mEos-2

Tags

His

Expression

Bacterial

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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mEos2-Lifeact-7

Plasmid

#54809

Purpose

Localization: Actin, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson, Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Lifeact-mEos2

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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