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Plasmid#180011PurposeDonor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld methodDepositorInsert2A-KalTA4; HE1A: BFP
UseSynthetic BiologyAvailable SinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-21-5p
Plasmid#103345PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBabe_puro_DEST_Flag_TNIK
Plasmid#45276DepositorAvailable SinceMay 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
Rescue Septin 11 mRNA
Plasmid#122022PurposeRescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.DepositorInsertSEPT11 (Sept11 Rat)
ExpressionMammalianMutationSilent mutations are in 5 consecutive codons (AUU…Available SinceFeb. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-TAPBPR-TM
Plasmid#178645PurposeMammalian expression of FLAG-tagged TAPBPR with MHC TMDepositorInsertTAPBPR (TAPBPL Human)
TagsFLAG and Signal/leader sequence from HLA class I …ExpressionMammalianMutationswitched transmembrane domain with that of MHC-IPromoterCMVAvailable SinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOD2047-bwmControl
Plasmid#100006PurposeMosSCI targeting vector expressing an ubiquitin ligase adaptor ZIF-1, serving as a control for pOD2046-bwmDEG. Expression is controlled by Pmyo-3 (body wall muscle specific).DepositorInsertsUseTargeting vector for mos1 transposon mediated sin…Available SinceSept. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRK5 Myc-tagged PSK
Plasmid#197149PurposeExpression of MYC-tagged PSK1-alpha / TAOK2 in mammalian cellsDepositorAvailable SinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pST1374-GCN4-D10A
Plasmid#113022PurposeExpresses GCN4-D10A in mammalian cellsDepositorAvailable SinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN_TT 3XFLAG-wtGATA6-3XAU1
Plasmid#72612PurposeGateway entry vector for an inducible N-terminally 3XFLAG-tagged and C-terminally AU1-tagged human wtGATA6DepositorInsertGATA binding protein 6 (GATA6 Human)
UseEntry vector for gateway cloningTags3XAU1 and 3XFLAGExpressionBacterialPromoterTRE tight promoterAvailable SinceJuly 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-let-7b-5p
Plasmid#103148PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-let-7b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-let-7b-5p target (MIRLET7B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-19a-3p
Plasmid#103323PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-19a-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-19a-3p target (MIR19A Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFRLuc-CoV2-delSS
Plasmid#177615PurposeDual luciferase reporter for SARS-CoV-2 programmed -1 ribosomal frameshifting. Slippery sequence (SS) deleted (negative control).DepositorInsertSARS-CoV-2 FSE
UseLuciferaseExpressionMammalianMutationSlippery sequence deletedAvailable SinceDec. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-SMYD3
Plasmid#85650Purposeretroviral expression of human SMYD3DepositorAvailable SinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTRY-YFP
Plasmid#15302DepositorInsertYFP
UseGateway entryAvailable SinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLV-SI-121
Plasmid#131126PurposeA-to-G base editing reporterDepositorInsertmutant EGFP
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterCMV promoterAvailable SinceSept. 23, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL3-IRES-miR34a-P2
Plasmid#64786PurposeLuciferase expression driven by miR-34a promoter P2DepositorInsertmiR-34a promoter P2 (MIR34A Human)
UseLuciferaseAvailable SinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSCV-IRES-GFP ASXL1 (1-479) 3x FLAG
Plasmid#81023PurposeCancer-associated ASXL1 truncation mutant in retroviral vectorDepositorInsertASXL1 (ASXL1 Human)
Tags3X FLAGExpressionMammalianMutationContains ASXL1 amino acids 1-479Promoter5’LTRAvailable SinceNov. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
J23100-mRFP1-331Bb
Plasmid#78271PurposemRFP1 behind constitutive promoter J23100 in pSEVA331Bb backboneDepositorInsertJ23100-mRFP1
Available SinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR Human CASP9 -2
Plasmid#198420PurposeExpresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter ProteinDepositorInsertHuman CASP9 Specific gRNA (CASP9 Human)
UseCRISPRTagsCas9/Orange Fluorescent Protein ReporterExpressionMammalianPromoterU6; CMVAvailable SinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR Human CASP9 -1
Plasmid#198419PurposeExpresses Human CASP9 Specific gRNA/Cas9 Complex and Reporter ProteinDepositorInsertHuman CASP9 Specific gRNA (CASP9 Human)
UseCRISPRTagsCas9/Orange Fluorescent Protein ReporterExpressionMammalianPromoterU6; CMVAvailable SinceApril 13, 2023AvailabilityAcademic Institutions and Nonprofits only