162,257 results

1. pAAV-hsyn-GRAB_Ado1.0

   Plasmid

   #153285

   Purpose

   Express the adenosine sensor GRAB_Ado1.0 in neurons

   Depositor

   Yulong Li

   Article

   Peng et al Science. 2020 Sep 4;369(6508). pii: 369/

   Insert

   Adenosine sensor GRAB_Ado1.0
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   hSyn

   Available since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSGKP-km

   Plasmid

   #117233

   Purpose

   A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae

   Depositor

   Quanjiang Ji

   Article

   Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-45b(+)-Tn5

   Plasmid

   #112112

   Purpose

   Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

   Depositor

   Frank Pugh

   Article

   Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

   Insert

   Tn5
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E54K E110K P242A L372P

   Promoter

   Available since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAX FLAG APP

   Plasmid

   #30154

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein (APP Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   wild type splice variant 751

   Promoter

   Available since

   July 29, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV-APOBEC1-YTH

   Plasmid

   #131636

   Purpose

   Expresses APOBEC1 fused to the YTH domain

   Depositor

   Kate Meyer

   Article

   Meyer et al Nat Methods. 2019 Sep 23. pii: 10.1038/s

   Insert

   APOBEC1 fused to YTH domain (YTHDF2 Rat, Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. rGluA2 (px330:2-guideCas9)

   Plasmid

   #169439

   Purpose

   Expression of Cas9 and 2 guides

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMD2.G-K47Q/R354Q

   Plasmid

   #207323

   Purpose

   VSV-G mutant with abolished receptor binding, but maintained fusogenic capacity

   Depositor

   Feng Zhang

   Article

   Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

   Insert

   VSV-G (VSIVgp4 Vesicular Stomatitis Virus)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   K47Q/R354Q

   Promoter

   CMV promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. CLYBL-TO-PAX7

   Plasmid

   #204346

   Purpose

   Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

   Depositor

   Ivo Lieberam

   Article

   Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

   Insert

   human PAX7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available since

   Oct. 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSFDuet-sumo-h-cGASCD(K427E/K428E)

   Plasmid

   #127162

   Purpose

   expression of truncated human cGAS protein

   Depositor

   Thomas Tuschl

   Article

   Lama et al Nat Commun. 2019 May 21;10(1):2261. doi:

   Insert

   MB21D1 (CGAS Human)
   

   Use

   Tags

   His-SUMO tag

   Expression

   Bacterial

   Mutation

   None

   Promoter

   Available since

   Oct. 2, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-CD80

    Plasmid

    #223603

    Purpose

    Lentiviral expression of human CD80 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD80 (CD80 Human)
    

    Use

    Lentiviral; mammalian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. 2X PRE TK luc

    Plasmid

    #11350

    Depositor

    Donald McDonnell

    Article

    Giangrande et al Mol Cell Biol. 2000 May . 20(9):3102-15.

    Insert

    PRE
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. GFP-PIPK1 gamma 90

    Plasmid

    #22299

    Depositor

    Pietro De Camilli

    Article

    Di Paolo et al Nature. 2002 Nov 7. 420(6911):85-9.

    Insert

    human phosphatidylinositol phosphate kinase type 1 gamma 90 (PIP5K1C Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLENTI_RPS10

    Plasmid

    #127129

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    RPS10 (RPS10 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K24R- please see depositor comments

    Promoter

    Available since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PB CAG-mTagBFP2-T2A-iCreERT2

    Plasmid

    #177308

    Purpose

    CAG-mTagBFP2-T2A-iCre-ERT2 piggyBac transposon

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCreERT2
    

    Use

    Cre/Lox; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)-HLA-Flag-natT1R3

    Plasmid

    #113950

    Purpose

    mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain

    Depositor

    Erik Procko

    Article

    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

    Insert

    T1R3 (TAS1R3 Human)
    

    Use

    Tags

    FLAG and Signal/leader sequence from HLA class I …

    Expression

    Mammalian

    Mutation

    residues 21-852

    Promoter

    Available since

    Feb. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHR-BRD4ΔN-mCherry-sspB

    Plasmid

    #121968

    Purpose

    Expresses fusion of disordered protein BRD4(462-1362), fluorescent protein mCherry, and sspB which upon light activation binds to iLID.

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BRD4 (BRD4 Human)
    

    Use

    Lentiviral

    Tags

    mCherry-sspB

    Expression

    Mammalian

    Mutation

    Deleted amino acids 1-461

    Promoter

    SFFV

    Available since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. NLS-Laconic/pCMV-myc-nuc

    Plasmid

    #46307

    Purpose

    a nuclear-targeted, genetically-encoded sensor for lactate

    Depositor

    Luis Felipe Barros

    Article

    Barros et al Front Cell Neurosci. 2013;7:27. doi: 10.

    Insert

    NLS-Laconic
    

    Use

    Lactate biosensor

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 2, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. moxGFP

    Plasmid

    #68070

    Purpose

    C-terminal fusion to moxGFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    moxGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-SUN1 GFP

    Plasmid

    #187682

    Purpose

    In vivo visualization of SUN1

    Depositor

    Mary Beckerle

    Insert

    SUN1 (Sun1 Mouse)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hsyn-Jaws-GFP-ER2

    Plasmid

    #65016

    Purpose

    AAV-mediated expression of Jaws-ER2 under the human synapsin promoter

    Depositor

    Edward Boyden

    Article

    Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

    Insert

    Jaws-GFP-ER2
    

    Use

    AAV

    Tags

    ER2 and GFP

    Expression

    Mammalian

    Mutation

    K200R W214F

    Promoter

    human synapsin

    Available since

    July 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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