We narrowed to 20,982 results for: OMP

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pETSUMO-TtAgocatdead

Plasmid

#210322

Purpose

Bacterial expression of the His- and SUMO-tagged catalytically inactive Argonaute protein of Thermus thermophilus

Depositor

Phillip Zamore

Article

Jolly et al Cell. 2020 Sep 17;182(6):1545-1559.e18.

Insert

TtAgo (TT_P0026 Thermus thermophilus)

Tags

6x His tag and SUMO tag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-NES-ZapCY2

Plasmid

#59016

Purpose

Cytosolic zinc-binding FRET biosensor

Depositor

Amy Palmer

Article

Qin et al ACS Chem Biol. 2013 Nov 15;8(11):2366-71

Insert

NES-ZapCY2 genetically encoded cytosolic Zn(II) sensor

Tags

Nuclear Exclusion Signal

Expression

Mammalian

Mutation

See comment

Promoter

CMV

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
RS18960_pMQ30_KO_Kan

Plasmid

#185393

Purpose

Non-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11

Depositor

Adam Arkin

Article

Sander et al Biotechnol Bioeng. 2024 Jan;121(1):139-1

Inserts

RR42_RS18960 upstream flanking homology region
RR42_RS18960 downstream flanking homology region

Available Since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-Flag-mPRMT7-GFP

Plasmid

#87104

Purpose

Express mouse PRMT7 with Flag-tag in mammalian cells

Depositor

Min Gyu Lee

Article

Lee et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

Protein arginine methyltransferase 7 (Prmt7 Mouse)

Tags

Flag

Expression

Mammalian

Promoter

EF1

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pOPS0253

Plasmid

#115505

Purpose

Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing gusA.

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Rlv3841pNifH pOGG082, gusA pOGG083, T-pharma pOGG003 assembled in pOGG026

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean-WW-NES

Plasmid

#84629

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean-WW-NES

Tags

6xHis, Cerulean, Nuclear export signal (NES), T7 …

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-BirA(R118G)-NES-YFP-STOP-L2

Plasmid

#127357

Purpose

gateway entry vector for expressing cytosolic BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NES, V5, and YFP

Mutation

R118G

Promoter

no promoter

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) neo with Smad5 CDS

Plasmid

#61800

Purpose

pcDNA3.1(+) with full length mouse Smad5 CDS

Depositor

Judy Lieberman

Article

Perdigao-Henriques et al Oncogene. 2015 Mar 23. doi: 10.1038/onc.

Insert

Smad5 CDS (Smad5 Mouse)

Expression

Mammalian

Available Since

May 14, 2015

Availability

Academic Institutions and Nonprofits only
pGEX6p-Meis1a

Plasmid

#78144

Purpose

bacterial expression of GST-Meis1a

Depositor

Francesco Blasi

Article

Dardaei et al Proc Natl Acad Sci U S A. 2014 Mar 11;11

Insert

Meis1a (Meis1 Mouse)

Tags

GST

Expression

Bacterial

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
OVA-miSFIT-8-G

Plasmid

#124675

Purpose

Tuning expression of cytosolic Ovalbumin coupled to EGFP

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

Cyto-Ovalbumin

Expression

Mammalian

Mutation

Truncated OVA for cytoplasmic expression

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pOPS0384

Plasmid

#133234

Purpose

Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

Depositor

Philip Poole

Article

Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

Insert

constructed with NoP (pOGG113), mCherry (EC15071) and T-pharma (pOGG003)

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

April 8, 2020

Availability

Academic Institutions and Nonprofits only

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MS2-adRNA (5'UAG)

Plasmid

#170126

Purpose

Lentiviral vector carrying 2 copies of the MS2 adRNA targeting a UAG at the 5' end of the ADAR2 deaminase domain in plasmids #170124 and 170125

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

MS2-ADAR2(5'UAG)-MS2

Use

Lentiviral

Expression

Mammalian

Promoter

Human U6 and mouse U6

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-EF1A-LivePAR(R163A)-Hygro

Plasmid

#187609

Purpose

EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

Depositor

Robert Sobol

Article

Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

Insert

LivePAR(R163A)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

R163A mutation in the WWE Domain

Promoter

EF1A

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-UAG site)

Plasmid

#170138

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(UAG)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX Syk(N)-SH2

Plasmid

#46520

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Syk(N) (SYK Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 7-114

Promoter

Tac

Available Since

July 12, 2013

Availability

Academic Institutions and Nonprofits only

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REEP5-2Strep

Plasmid

#210350

Purpose

Expresses REEP5 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP5 (REEP5 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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BoxB-MS2 adRNA (RAB7A-ACG site)

Plasmid

#170155

Purpose

AAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editing

Depositor

Prashant Mali

Article

Katrekar et al Elife. 2022 Jan 19;11. pii: 75555. doi:

Insert

BoxB-RAB7A(ACG)-MS2

Use

AAV

Expression

Mammalian

Promoter

Human U6

Available Since

March 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGAD-Atg17

Plasmid

#121386

Purpose

For Yeast-two-hybrid analysis

Depositor

Daniel Klionsky

Article

Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

Insert

ATG17

Expression

Yeast

Promoter

ADH1 promoter

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pfastbac/mBmal62-447

Plasmid

#47335

Purpose

mBmal fragment cloned into pfastbac and crystallized

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Mutation

aa 62-447

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pRenilla (GB0056)

Plasmid

#68198

Purpose

Provides the CDS of the Renilla protein as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Renilla coding region

Use

Luciferase and Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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