We narrowed to 10,965 results for: T7

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FLuc-N-HiBiT

Plasmid

#207117

Purpose

Bacterially expressed HiBiT-FLuc for Ni-NTA purification

Depositor

James Inglese

Article

Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

Insert

luciferin 4-monooxygenase

Tags

HiBiT Tag and His-tag with thrombin site

Expression

Bacterial

Mutation

Codon optmization for expression in BL21DE3

Promoter

T7 Promotor

Available Since

May 13, 2025

Availability

Academic Institutions and Nonprofits only

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pETM33_CRK_SH3

Plasmid

#178472

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CRK_SH3 (CRK Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-Erythropoietin

Plasmid

#182422

Purpose

Bacterial expression of mNeongreen-Erythropoietin fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-eythropoietin (EPO Human)

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pETM33_MDM2_SWIB

Plasmid

#178481

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

MDM2_SWIB (MDM2 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pBK086

Plasmid

#183145

Purpose

pET-6xHis-MBP-TEV-MapTIR-APAZ/MapAgo - Heterolgous MapSPARTA expression

Depositor

Daan Swarts

Article

Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

Insert

MapSPARTA operon (6xHis-MBP-MapTIR-APAZ and MapAgo)

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pET21 Catalytic Domain of MALT1

Plasmid

#48970

Purpose

Expresses catalytic domain of MALT1 in e.coli AA 329-566

Depositor

Guy Salvesen

Article

Hachmann et al Biochem J. 2012 Apr 1;443(1):287-95. doi

Insert

MALT1 (MALT1 Human)

Tags

His

Expression

Bacterial

Mutation

Contains AA 329-566

Promoter

T7

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-sACt-mCherry-NLS

Plasmid

#138214

Purpose

Encodes residues 1-469 of rat soluble adenylyl cyclase (ADCY10) fused to mCherry; nuclear targeted.

Depositor

Jin Zhang

Article

Sample et al Nat Chem Biol. 2012 Feb 26;8(4):375-82.

Insert

sACt-mCherry-NLS (Adcy10 Rat)

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-Etanercept

Plasmid

#182420

Purpose

Bacterial expression of mNeongreen-Etanercept fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-Etanercept (TNFRSF1B Human)

Tags

mNeongreen

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSMT3-FBF2

Plasmid

#166972

Purpose

Expresses FBF-2 in E. coli for purification and crystallization studies

Depositor

Traci Hall

Article

Bhat et al Elife. 2019 Jan 17;8. pii: 43788. doi: 1

Insert

FBF-2 (fbf-2 Nematode)

Tags

His-SUMO

Expression

Bacterial

Mutation

FBF-2 PUM RNA binding domain; amino acids 164-575

Promoter

T7

Available Since

April 29, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX6P1-human full-length LIC1

Plasmid

#74597

Purpose

used for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag

Depositor

Ron Vale

Article

Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

Insert

full length human dynein light intermediate chain 1 (DYNC1LI1 Human)

Tags

N-terminal GST and Strep II

Expression

Bacterial

Promoter

T7

Available Since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-8

Plasmid

#172772

Purpose

Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-8

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaM-2

Plasmid

#172768

Purpose

Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaM-2

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pBK092

Plasmid

#183146

Purpose

pET-6xHis-MBP-TEV-CrtTIR-APAZ/CrtAgo - Heterologous CrtSPARTA expression

Depositor

Daan Swarts

Article

Koopal et al Cell. 2022 Apr 28;185(9):1471-1486.e19.

Insert

CrtSPARTA operon (6xHis-MBP-CrtTIR-APAZ and CrtAgo)

Tags

6xHis and MBP

Expression

Bacterial

Promoter

T7

Available Since

April 21, 2022

Availability

Academic Institutions and Nonprofits only

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pETM33_CLIP_CAP_Gly

Plasmid

#178470

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CLIP_CAP_Gly (CLIP1 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

Plasmid

#123353

Purpose

Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

Depositor

Jin Zhang

Article

Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

Insert

Cerulean3-Cerulean3-FLARE-Cameleon

Tags

6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

Expression

Mammalian

Promoter

CMV

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_hGH

Plasmid

#182410

Purpose

Bacterial expression of human growth hormone

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

hGH (GH1 Human)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pET28-Mff(1-61)-PP-GST

Plasmid

#73042

Purpose

Bacterial expression of mouse Mff residues 1-61 with C-terminal GST-tag and PreScission protease cleavage site

Depositor

David Chan

Article

Liu et al Mol Biol Cell. 2015 Dec 1;26(24):4466-77

Insert

Mff(1-61) (Mff Mouse)

Tags

GST-tag and PreScission protease site

Expression

Bacterial

Mutation

Truncation containing amino acids 1-61

Promoter

T7

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pAR127_StrepI_m(KS_MAT)_mouseL_ACP_H8_pET22b

Plasmid

#122850

Purpose

Expresses a designed construct of the condensing part fused to the ACP domain of murine type I fatty acid synthase (FASN) in Escherichia coli

Depositor

Martin Grininger

Article

Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

Insert

m(KS_MAT)_mouseL_ACP (Fasn Mouse)

Tags

His8 tag and Twin-Strep tag

Expression

Bacterial

Mutation

wildtype

Promoter

T7 promoter

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pET-21a_2xNLS_LbCpf1_protein_expression

Plasmid

#114366

Purpose

Protein expression plasmid for 2xNLS LbCpf1 in pET-21a backbone

Depositor

Scot Wolfe

Article

Xu et al Blood. 2019 Jan 31. pii: blood-2019-01-8

Insert

2xNLS_LbCpf1

Use

CRISPR

Tags

NLS

Expression

Bacterial

Promoter

T7

Available Since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pETM41_KPNA4_Arm

Plasmid

#178478

Purpose

Bacterial expression of human domain with His-tag and MBP tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

KPNA4_Arm (KPNA4 Synthetic, Human)

Tags

His-MBP

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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