We narrowed to 9,902 results for: UTY

1. PhyB-mCherry-Spc72

   Plasmid

   #51582

   Purpose

   Constitutively express spindle pole body localized PhyB in Saccharomyces cerevisiae

   Depositor

   Chao Tang

   Article

   Yang et al Mol Biol Cell. 2013 Aug;24(15):2419-30.

   Insert

   PhyB (PHYB Mustard Weed)
   

   Use

   Tags

   Spc72 and mCherry

   Expression

   Yeast

   Mutation

   1-908aa only

   Promoter

   ADH1pr

   Available Since

   Aug. 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSLIK-Venus-TmiR-Luc

   Plasmid

   #25739

   Purpose

   Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

   Depositor

   Iain Fraser

   Article

   Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

   Insert

   Luciferase miR-shRNA
   

   Use

   Lentiviral and RNAi

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 1, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAFMW-Nbr-D435A/E437A

   Plasmid

   #40829

   Purpose

   Expresses Flag, Myc tagged mutant Nbr in Drosophila cells

   Depositor

   Phillip Zamore

   Article

   Han et al Curr Biol. 2011 Nov 22;21(22):1878-87. E

   Insert

   Nbr D435A/E437A (Nbr Fly)
   

   Use

   Tags

   3xFLAG and 6xMyc

   Expression

   Insect

   Mutation

   D435A/E437A, part of the DEDD motif; mutations p…

   Promoter

   Actin5C

   Available Since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pMRE-Tn5-151

   Plasmid

   #118533

   Purpose

   miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mTurquoise2
   

   Use

   Minitn5 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. PB-TAC-OKMS-Cas9

   Plasmid

   #120353

   Purpose

   piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.

   Depositor

   Knut Woltjen

   Article

   Kagawa et al Stem Cell Reports. 2018 Dec 28. pii: S22

   Insert

   OKMS cassette
   

   Use

   CRISPR; Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Klf4 [10-483]

   Promoter

   Available Since

   April 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMRE-Tn7-137

   Plasmid

   #118555

   Purpose

   miniTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

   Depositor

   Mitja Remus-Emsermann

   Article

   Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

   Insert

   mClover3
   

   Use

   Minitn7 delivery vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFPm-DAD M1041A

   Plasmid

   #25411

   Depositor

   Arthur Alberts

   Article

   Alberts et al J Biol Chem. 2001 Jan 26. 276(4):2824-30

   Insert

   Diap3 (Diaph3 Mouse)
   

   Use

   Tags

   EGFP, His, and Myc

   Expression

   Mammalian

   Mutation

   Alanine substitution at critical residue in DAD t…

   Promoter

   Available Since

   Oct. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pJM529 EF1α 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA in TUPV5

   Plasmid

   #161555

   Purpose

   Constitutive expression of 3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA under the EF1α promoter

   Depositor

   Joshua Leonard

   Article

   Muldoon et al Sci Adv. 2021 Feb 19;7(8). pii: 7/8/eabe

   Insert

   3xFLAG-NLS-DsDed-intC-ZF10-NLS-HA
   

   Use

   Synthetic Biology

   Tags

   3x-FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLIX402 caPHF2

   Plasmid

   #91701

   Purpose

   inducible lentiviral expression of constitutively active PHF2

   Depositor

   Bob Weinberg

   Article

   Pattabiraman et al Science. 2016 Mar 4;351(6277):aad3680. d

   Insert

   PHF2 (PHF2 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   caPHF2; S757D, S899D, S954D, S1056D

   Promoter

   TRE promoter, Tet ON

   Available Since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

10. XW17

    Plasmid

    #65835

    Purpose

    Punc-4-QF-3'UTR-SL2-mCherry-unc-54 (The endogenous SphI, KpnI and SacI sites in QF are removed for the convenience of cloning, most sites are compatible with pPD95.77)

    Depositor

    Kang Shen

    Article

    Wei et al Nat Methods. 2012 Mar 11;9(4):391-5. doi

    Insert

    OR74A quinic acid utilization activator (qa-1F)
    

    Use

    Tags

    mCherry

    Expression

    Worm

    Mutation

    The endogenous SphI, KpnI and SacI sites in QF ar…

    Promoter

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPS0380

    Plasmid

    #133230

    Purpose

    Reporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.

    Depositor

    Philip Poole

    Article

    Mendoza-Suarez et al Proc Natl Acad Sci U S A. 2020 May 5;117

    Insert

    constructed with constitutive promoter Rlv3841pnifH (pOGG082), mCherry (EC15071) and T-pharma (pOGG003)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available Since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCXLE-dCas9VPP300-T2A-GFP-shP53

    Plasmid

    #102896

    Purpose

    EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

    Depositor

    Timo Otonkoski

    Article

    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

    Insert

    dCas9VPP300-T2A-GFP-shP53
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A

    Promoter

    CAG

    Available Since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSLIK-Neo-TmiR-Luc

    Plasmid

    #25743

    Purpose

    Control lentiviral vector with Tet-based inducible expression of Luciferase miR30-based shRNA, constitutive Neomycin resistance gene coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    Luciferase miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 1, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1_nV5-TAOK2

    Plasmid

    #173000

    Purpose

    constitutive expression of V5-tagged TAOK2

    Depositor

    Michael Wehr

    Article

    Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

    Insert

    V5-TAOK2 (TAOK2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBlastR-5xUAS-sfGFP-MiniWhite

    Plasmid

    #165910

    Purpose

    Blasticidin resistant 5xUAS sfGFP Mini-White CDS response vector. Contains an Alpha level GB2.0 entry point for addition of custom assemblies utilizing blue-white bacterial colony screening

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Selectable UAS Response Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMRE-Tn5-144

    Plasmid

    #118528

    Purpose

    miniTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteria

    Depositor

    Mitja Remus-Emsermann

    Article

    Schlechter et al Front Microbiol. 2018 Dec 12;9:3052. doi

    Insert

    mOrange2
    

    Use

    Minitn5 delivery vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSB1C3_YPet-PCP (y-plasmid)

    Plasmid

    #127637

    Purpose

    Encodes a fluorecent protein with an RNA binding peptide: YPet-PCP.Expression with constitutive E. coli RNAP promoter (J23106), ribozyme RiboJ and RBS BBa_B0034.

    Depositor

    Friedrich Simmel

    Article

    Schwarz-Schilling et al Nano Lett. 2018 Apr 11;18(4):2650-2657.

    Insert

    YPet
    

    Use

    Synthetic Biology

    Tags

    RNA binding peptide: PP7 coat protein (PCP)

    Expression

    Bacterial

    Mutation

    Promoter

    pJ21306

    Available Since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1

    Plasmid

    #56658

    Purpose

    expresses constitutively active human PKCi with a deletion on the PB1 domain

    Depositor

    Pedro Salas

    Article

    Forteza et al Biol Open. 2013 Oct 8;2(11):1264-9. doi:

    Insert

    Human PKCi (PRKCI Human)
    

    Use

    Lentiviral

    Tags

    V5/His

    Expression

    Mutation

    A120E and deletion of PB1 domain (aa 24–98)

    Promoter

    CMV

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

19. DT034A

    Plasmid

    #159395

    Purpose

    Constitutive LuxR expression, controlled under E.coli hybrid (J23117-J23106) promoter, and inducible Perfringolysin O (PFO) expression, controlled under V. fischeri HSL-regulated pLux (luxPR) promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.J23117/J23106-LuxR-pr.LuxR-PFO-pdt
    

    Use

    Synthetic Biology

    Tags

    FLAG/FLAG and pdt#3 from Collins Nat. Biotech. 20…

    Expression

    Bacterial

    Mutation

    Promoter

    J23117 and pLux promoter

    Available Since

    March 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. SIN40C.SFFV.mTagBFP2.let-7c

    Plasmid

    #169310

    Purpose

    Constitutive overexpression of let-7c with mTagBFP2 as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    let-7c (MIRLET7C Human)
    

    Use

    Lentiviral

    Tags

    TagBFP2

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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