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We narrowed to 128,269 results for: CIL

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Showing: 8481 - 8500 of 128269 results

  1. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Publication
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Expression
    Mammalian
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

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  3. pCK302

    Plasmid
    #87768
    Purpose
    As pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be used
    Depositor
    John Heap
    Publication
    Kelly et al ACS Synth Biol. 2016 Oct 21;5(10):1136-1
    Inserts
    PrhaBAD-sfGFP
    rhaS from E. coli with its native RBS from E. coli
    Use
    Synthetic Biology
    Tags
    6xHis Tag
    Expression
    Bacterial
    Promoter
    PrhaBAD rhamnose-inducible promoter from E. coli …
    Available since
    May 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    87768-plasmid-map-sequence-id-171131

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  6. EB3N-VVDfast-mCl3-iLID

    Plasmid
    #190165
    Purpose
    Expresses EB3N-VVDfast-mCl3-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.
    Depositor
    Anna Akhmanova
    Publication
    Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EB3N-VVDfast-mClover3-iLID (MAPRE3 Synthetic, Human)
    Tags
    -VVDfast-mClover3-iLID
    Expression
    Mammalian
    Mutation
    1-189 aa from EB3
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    190165-plasmid-map-sequence-id-374287

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  7. pCAG R2pH-LAMP1-3xFLAG

    Plasmid
    #157940
    Purpose
    expresses ratiometric sensor of lysosomal lumenal pH
    Depositor
    Massimiliano Stagi
    Publication
    Ponsford et al Autophagy. 2020 Jun 9:1-19. doi: 10.1080
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    R2pH-LAMP1-3xFLAG (Lamp1 Mouse)
    Tags
    3XFLAG, mCherry, and pHluorin
    Expression
    Mammalian
    Promoter
    Chick beta actin pomoter
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    157940-plasmid-map-sequence-id-315065

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  8. SUV[SET]-dCas9

    Plasmid
    #100088
    Purpose
    Catalytic domain [SET] of human SUVAR39H1 fused to N-terminus of dCas9; pCDNA3 vector backbone, mammalian expression
    Depositor
    David Segal
    Publication
    O'Geen et al Nucleic Acids Res. 2017 Sep 29;45(17):99
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SUVAR39H1 (SUV39H1 Human)
    Use
    CRISPR
    Tags
    3XFLag-NLS-SUV[SET]-dCas9-NLS
    Expression
    Mammalian
    Mutation
    deleted aa 1-76
    Promoter
    CMV
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    100088-plasmid-map-sequence-id-197559

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  11. pCMV5 HA-TGFBRII

    Plasmid
    #19158
    Depositor
    Joan Massague
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Transforming growth factor, beta receptor II (TGFBR2 Human)
    Tags
    HA (YDVPDYASL)
    Expression
    Mammalian
    Mutation
    PCR based mutagenesis
    Available since
    Oct. 1, 2008
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    19158-plasmid-map-sequence-id-364716

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  14. pl-Synapsin-YFP-NaVII-III

    Plasmid
    #91246
    Purpose
    Expresses YFP-NaVII-III from the Synapsin promoter, for neuron-specific and activity-independent live label of the Axon Initial Segment (AIS)
    Depositor
    Matthew Grubb
    Publication
    Dumitrescu et al Front Cell Neurosci. 2016 Nov 23;10:268.
    Insert
    Nav1.2 (Scn2a Rat)
    Use
    Lentiviral
    Tags
    YFP
    Expression
    Mammalian
    Mutation
    The insert codes only for the intracellular loop …
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    91246-plasmid-map-sequence-id-180624

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  15. AICSDP-13:FBL-mEGFP

    Plasmid
    #87427
    Purpose
    Homology arms and linker-mEGFP sequence for C-terminus tagging of human FBL
    Depositor
    Allen Institute for Cell Science
    Publication
    Roberts et al Mol Biol Cell. 2017 Oct 15;28(21):2854-2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FBL Homology Arms with linker-mEGFP (FBL Human)
    Use
    CRISPR; Donor template
    Tags
    linker-mEGFP
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Available since
    March 15, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    87427-plasmid-map-sequence-id-173980

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  16. pcDNA3-N-Flag-NLRP3 711-1034

    Plasmid
    #75141
    Purpose
    Expresses N-terminal Flag-tagged amino acids 711-1034 of NLRP3 (the LRR domain) in mammalian cells
    Depositor
    Bruce Beutler
    Publication
    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:
    Insert
    NLR family, pyrin domain containing 3 (Nlrp3 Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    fragment encoding amino acids 711-1034
    Promoter
    CMV
    Available since
    May 26, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    75141-plasmid-map-sequence-id-148062

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  17. pUBC_mCherry_serinemod_E488QADAR_p2A_yGFP

    Plasmid
    #154786
    Purpose
    Expresses mCherry fused to hyperactive ADAR catalytic domain, control plasmid for HyperTRIBE
    Depositor
    Robert Singer
    Publication
    Biswas et al iScience. 2020 Jun 28;23(7):101318. doi:
    Insert
    Adenosine deaminse (Adar Synthetic, Fly)
    Use
    Lentiviral
    Tags
    GFP, V5, and mCherry
    Expression
    Mammalian
    Mutation
    Synonymized serine to prevent autoediting of ADAR…
    Promoter
    UBC
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    154786-plasmid-map-sequence-id-306310

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Showing: 8481 - 8500 of 128269 results