We narrowed to 57,299 results for: plasmid
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Plasmid#122186PurposeBacterial genome editing plasmid with spectinomycin resistance markerDepositorTypeEmpty backboneUseCRISPRExpressionBacterialAvailable sinceMarch 12, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pMAL-SH3(2)-5R
Plasmid#112089PurposeBacterial expression plasmid containing His and MBP tags for 5 repeats of the second SH3 domain from human NCK1.DepositorInsertSH3(2)-5R (NCK1 Human)
TagsHis6 and MBPExpressionBacterialMutationconstruct contains only repeats of the second SH3…PromoterLacAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28b-T7-hAsCas12a-NLS(nucleoplasmin)-6xHis (BPK3541)
Plasmid#114069PurposeT7 promoter bacterial expression plasmid for human codon optimized AsCas12a with C-terminal NLS and His-tagDepositorInserthuman codon optimized AsCas12a with C-terminal NLS and His-tag
TagsNLS(nucleoplasmin) and 6xHisExpressionBacterialAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEEK2
Plasmid#83492PurposeExpression plasmid for conjugational transfer into cyanobacteriaDepositorTypeEmpty backboneUseSynthetic BiologyTagsStrep-tagExpressionBacterialPromoterPtrc coreAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LSB-hsa-miR-21-5p
Plasmid#103345PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-21-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP mCherry-ApoB-EGFP
Plasmid#112859PurposePlasmid bearing the editing target of APOBEC1 fused between the mCherry cds and the EGFP one. In presence of APOBEC1, the CAA codon in ApoB is edited to a Stop codon (UAA), leading to loss of EGFPDepositorInsertmCherry-apoB-EGFP
ExpressionMammalianPromoterCMV promoterAvailable sinceOct. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET51b(+)_H-Luc
Plasmid#113924PurposePlasmid encoding the bacterial expression of the chimeric HaloTag7-NanoLucCP fusion dubbed H-Luc, for BRETDepositorInsertH-Luc
UseLuciferaseExpressionBacterialPromoterT7Available sinceSept. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pF CAG BFP2 IRES neo
Plasmid#108175PurposeExpresses a blue fluorescent protein (BFP2) in mammalian cells along with conferring resistance to G418DepositorInsertBlue fluorescent protein (BFP2)
UseLentiviral; Fluorescent proteinExpressionMammalianPromoterCAGAvailable sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
Pdgfra-T2A-H2B-eGFP donor
Plasmid#113120PurposeDonor plasmid for knock-in T2A-H2B-eGFP to the c-terminal of mouse Pdgfra coding sequenceDepositorInsertPdgfra donor region including homology arms and T2A-H2B-eGFP (Pdgfra Synthetic, Mouse)
UseSynthetic BiologyAvailable sinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTXRE5-Z
Plasmid#112509PurposeReporter plasmid, responsive to activated Ah receptor/ArntDepositorInsertRW95-3 with 5x xenobiotic response elements (lacZ Synthetic, E.coli)
ExpressionYeastPromoter5 XREs+ minimal Cytochrome CAvailable sinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
sgPBRM1-2- LentiCRISPRv2
Plasmid#107404PurposeLentiviral expression of Cas9 and gRNA targeting PBRM1DepositorInsertgRNA targeting PBRM1 (PBRM1 Human)
UseLentiviralAvailable sinceJune 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_LP1B_St1Cas9_LMD-9_SpA_U6_sgRNA
Plasmid#110624PurposeA single vector AAV-Cas9 system containing a liver-specific promoter with Cas9 from Streptococcus thermophilus CRISPR1 (St1Cas9 LMD-9) and its U6-driven sgRNADepositorInsertsSt1Cas9 LMD-9
sgRNA for St1Cas9
UseAAV, CRISPR, and Mouse TargetingTagsSV40 NLSExpressionMammalianPromoterLP1B and hU6Available sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-T2A-Puro_h53BP1_gRNA_D
Plasmid#110302PurposeExpresssion of Cas9-T2A-puromycin resistant gene and a gRNA targeting exon 10 of human 53BP1DepositorInsertp53-binding protein 1 (TP53BP1 Human)
ExpressionMammalianAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB1-spect-PthrC3_8-eGFP
Plasmid#107411PurposeThis plasmid contain promoter PthrC3_8, which is used for study about Short, Auto-inducible Promoters for Well-Controlled Protein Expression in Escherichia coli.DepositorInserteGFP
ExpressionBacterialPromoterPthrC3_8Available sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM489-Snap
Plasmid#108437PurposeORBIT integrating plasmid for C-terminal tagging with SNAP tag.DepositorInsertattB-SNAP tag
UseOrbit integrating plasmidAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKM492-Myc-His
Plasmid#109283PurposeORBIT integrating plasmid for C-terminal tagging with Myc-His tagDepositorInsertattB-Myc-His
UseOrbit integrating plasmidAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBAD-K-GECO1
Plasmid#105864PurposeBacterial expression plasmid for red fluorescent Ca2+ indicator K-GECO1DepositorInsertK-GECO1
Tags6-His TagExpressionBacterialAvailable sinceApril 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMSCVpuro-Venus
Plasmid#96940PurposeRetroviral plasmid containing Venus for labeling of cellsDepositorInsertVenus Fluorescent Protein
ExpressionMammalianAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
NRIP1_pCSdest
Plasmid#53805DepositorAvailable sinceMarch 27, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
YIplac211-yoHaloTag-TLG1
Plasmid#105277PurposePlasmid for pop-in/pop-out replacement of Tlg1 with yoHaloTag-Tlg1.DepositorInsertTlg1 (TLG1 Budding Yeast)
TagsyoHaloTagExpressionYeastMutationHaloTag has yeast optimized codonsAvailable sinceJan. 26, 2018AvailabilityAcademic Institutions and Nonprofits only