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pcDNA3.2/V5 mmu-mir-375

Plasmid

#26336

Depositor

David Bartel

Article

Chiang et al Genes Dev. 2010 May 15. 24(10):992-1009.

Insert

mmu-mir-375 (Mir375 Mouse)

Expression

Mammalian

Available Since

Sept. 29, 2010

Availability

Academic Institutions and Nonprofits only
[#GS5-RV] MitoTRACER-Donor

Plasmid

#233505

Purpose

Retroviral construct of the MitoTRACER genetic reporter to be expressed in the donor cells

Depositor

Simon Grelet

Article

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

MitoTRACER-Donor

Use

Retroviral and Synthetic Biology

Tags

GFP11 and HA Tag

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-FLEX-eNpHR-EYFP

Plasmid

#89876

Purpose

To generate AAV virus that will express eNpHR-EYFP in Cre-dependent recombination under control of tetO promoter

Depositor

Hyungbae Kwon

Article

Lee et al Nat Methods. 2017 May;14(5):495-503. doi

Insert

eNpHR=EYFP

Use

AAV

Tags

EYFP

Expression

Mammalian

Promoter

TetO

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRY-HF1-ABE8e

Plasmid

#185670

Purpose

Expresses SpRY-HF1-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY-HF1

Use

CRISPR

Expression

Mammalian

Mutation

SpRY-HF1 D10A

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-wtTnsC

Plasmid

#234732

Purpose

Wild-type human-codon optimized PseTnsC in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PseTnsC

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCMV-wtTnsAB

Plasmid

#234731

Purpose

Wild-type human-codon optimized PseTnsAB in mammalian cells

Depositor

David Liu

Article

Witte et al Science. 2025 May 15;388(6748):eadt5199.

Insert

PseTnsAB

Expression

Mammalian

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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RFP-PASS

Plasmid

#193971

Purpose

Monitoring the production of phosphatidic acid with RFP-PASS (phosphatidic acid biosensor with superior sensitivity)

Depositor

Guangwei Du

Article

Zhang et al Mol Cell Biol. 2014 Jan;34(1):84-95. doi

Insert

PA biosenor

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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Nme2Cas9_pCDest

Plasmid

#119923

Purpose

Nme2Cas9 CMV-driven mammalian expression plasmid

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Tags

NLS-3xHA-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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PIEZO1-HaloTag

Plasmid

#207834

Purpose

PIEZO1 fusion for biochemistry and live imaging

Depositor

Charles Cox

Article

Zhou et al Science. 2023 Aug 18;381(6659):799-804.

Insert

PIEZO1 (PIEZO1 Human)

Tags

HaloTag

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 15, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO Puro DEST (670-1)

Plasmid

#17293

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pZR112_Lenti-SFFV-mCherry-2A-dCas9-VP64

Plasmid

#180263

Purpose

Lentiviral expression vector for dCas9-VP64 in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Blast DEST (706-1)

Plasmid

#17451

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBT281

Plasmid

#122611

Purpose

express evoAPOBEC1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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YFP B1 in pcDNA3.1

Plasmid

#36397

Depositor

Narasimhan Gautam

Article

Azpiazu et al J Biol Chem. 2004 Jun 25;279(26):27709-1

Insert

G protein B1 (GNB1 Bovine)

Tags

YFP

Expression

Mammalian

Promoter

cmv

Available Since

April 4, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.2/EV

Plasmid

#120833

Purpose

Control vector, 20nt random sequence between gateway recombination sites

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Puro DEST (w529-2)

Plasmid

#19068

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Puro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pZR071_SFFV-dCas9-mCherry-KRAB

Plasmid

#180264

Purpose

Lentiviral expression vector for dCas9-KRAB in mammalian cells

Depositor

Alexander Marson

Article

Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

KRAB and mCherry

Expression

Mammalian

Mutation

Nuclease dead Cas9

Promoter

SFFV

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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CRY2olig-mCherry

Plasmid

#60032

Purpose

Expresses a fusion of CRY2PHR E490G with mCherry

Depositor

Chandra Tucker

Article

Taslimi et al Nat Commun. 2014 Sep 18;5:4925. doi: 10.

Insert

CRY2PHR E490G (CRY2 Mustard Weed)

Expression

Mammalian

Mutation

E490G

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only

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pC014 - LwCas13a-msfGFP

Plasmid

#91902

Purpose

Expresses active LwCas13a for mammalian RNA targeting

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LwCas13a

Use

CRISPR

Tags

NLS and msfGFP-NLS-3xHA

Expression

Mammalian

Promoter

EF1a

Available Since

Dec. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

Plasmid

#180280

Purpose

The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI sgRNA (backbone) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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