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We narrowed to 10,356 results for: plasmids 101

Showing: 8561 - 8580 of 10356 results

  3. pcDNA5 rtTA BirA eIF4G

    Plasmid
    #158789
    Purpose
    pcDNA5 based transfection plasmid for the exogenous expression of N-terminal BirA-Flag-tagged eIF4G1 lacking the microexon for generation of N2A FlpIn cell lines for BioID
    Depositor
    Benjamin Blencowe
    Article
    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1
    Insert
    eIF4G1
    Use
    Tags
    3xFlag and BirA*
    Expression
    Mammalian
    Mutation
    Promoter
    miniCMV
    Available Since
    Aug. 27, 2020
    Availability
    Academic Institutions and Nonprofits only
    158789-plasmid-map-sequence-id-309804

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  4. pLCKO-CMV-Blasticidin-P2A-3xHA-TMEM106B_Hamster

    Plasmid
    #238131
    Purpose
    This plasmid encodes the full-length hamster (Mesocricetus auratus) TMEM106B protein fused with a 3xHA tag, along with an N-terminal blasticidin selection marker.
    Depositor
    Dirk Daelemans
    Article
    Baggen et al Cell. 2023 Aug 3;186(16):3427-3442.e22.
    Insert
    TMEM106B
    Use
    Lentiviral
    Tags
    Blasticidin-P2A-3xHA
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    June 18, 2025
    Availability
    Academic Institutions and Nonprofits only
    238131-plasmid-map-sequence-id-476564

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  8. Δ1-60MEK1C222

    Plasmid
    #164641
    Purpose
    Bacterial expression plasmid for His6-ΔMEK1C222
    Depositor
    Ben Davis
    Article
    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00
    Insert
    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    Use
    Tags
    His6-tag
    Expression
    Bacterial
    Mutation
    S222C/C277S/C376S; residues 1-60 at N-terminus we…
    Promoter
    T7
    Available Since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164641-plasmid-map-sequence-id-328776

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  9. Δ1-60MEK1C218

    Plasmid
    #164643
    Purpose
    Bacterial expression plasmid for His6-ΔMEK1C218
    Depositor
    Ben Davis
    Article
    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00
    Insert
    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    Use
    Tags
    His6-tag
    Expression
    Bacterial
    Mutation
    S218C/C277S/C376S; residues 1-60 at N-terminus we…
    Promoter
    T7
    Available Since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164643-plasmid-map-sequence-id-329215

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  10. Δ1-60MEK1C218C222

    Plasmid
    #164645
    Purpose
    Bacterial expression plasmid for His6-ΔMEK1C218C222
    Depositor
    Ben Davis
    Article
    Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00
    Insert
    N-terminal truncated mitogen-activated protein kinase kinase 1 (MAP2K1 Human)
    Use
    Tags
    His6-tag
    Expression
    Bacterial
    Mutation
    S218C/S222C/C277S/C376S; residues 1-60 at N-termi…
    Promoter
    T7
    Available Since
    May 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    164645-plasmid-map-sequence-id-329217

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  16. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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  17. G12-CASE

    Plasmid
    #190714
    Purpose
    Encodes a BRET-based activity sensor for the heterotrimeric G protein G12. Composed of the subunits G alpha 12 (GNA12) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).
    Depositor
    Gunnar Schulte
    Article
    Schihada et al J Biol Chem. 2022 Aug 3:102328. doi: 10.
    Inserts
    GNB3 (GNB3 Human)
    GNGT9
    GNA12 (GNA12 Human)
    Use
    Luciferase
    Tags
    Nluc is inserted at A133/F134 within GNA12 and cp…
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    Dec. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    190714-plasmid-map-sequence-id-379753

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Showing: 8561 - 8580 of 10356 results