We narrowed to 4,840 results for: phy

1. pIntegrase_8

   Plasmid

   #60579

   Purpose

   plasmid contains the integrase gene under the control of the arabinose-inducible promoter, Pbad

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Integrase_8
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli

   Promoter

   pBAD

   Available since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMH48

   Plasmid

   #131861

   Purpose

   Produces holo-Cph1* in E. coli

   Depositor

   Wilfried Weber

   Article

   Horner et al ACS Synth Biol. 2019 Sep 26. doi: 10.102

   Inserts

   Cph1(1-514,Y263F)-RGD-His6-Cys
   HO1
   PcyA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter-1 and T7 promoter-2

   Available since

   Oct. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRiA4-VIR

   Plasmid

   #138193

   Purpose

   Virulence helper compatible with pCambia binary vectors.

   Depositor

   Qi-Jun Chen

   Article

   Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

   Insert

   vir gene cluster
   

   Use

   Vir gene expression

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pG108 - K

   Plasmid

   #178039

   Purpose

   Bacteroides - Escherichia coli shuttle vector with TetQ and Kanamycin selection markers

   Depositor

   Janina Lewis

   Article

   Jones et al Mol Oral Microbiol. 2020 Aug;35(4):181-1

   Insert

   Kanamycin resistance
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   ErmAM has been replaced with Kanamycin for select…

   Promoter

   Available since

   Dec. 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSKI015

   Plasmid

   #11570

   Depositor

   Detlef Weigel

   Article

   Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

   Insert

   CaMV 35S enhancers
   

   Use

   Activation tagging

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 4, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. gamma Synuclein-VC

   Plasmid

   #89734

   Purpose

   expresses human gamma Synuclein fused to the C-terminal part of Venus

   Depositor

   Tiago Outeiro

   Article

   Eckermann et al Biochim Biophys Acta. 2015 Aug;1852(8):1

   Insert

   SNCG (SNCG Human)
   

   Use

   Tags

   C-terminal part of Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pALOD4

   Plasmid

   #111026

   Purpose

   Expresses domain 4 of anthrolysin O cholesterol sensor, has N-terminal His6-tag and two point mutations, S404C and C472A

   Depositor

   Arun Radhakrishnan

   Article

   Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

   Insert

   Domain 4 of Anthrolysin O
   

   Use

   Tags

   His6 tag

   Expression

   Bacterial

   Mutation

   Mutations: S404C, C472A

   Promoter

   T7

   Available since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSKI074

   Plasmid

   #11571

   Depositor

   Detlef Weigel

   Article

   Weigel et al Plant Physiol. 2000 Apr . 122(4):1003-13

   Insert

   CaMV 35S enhancers
   

   Use

   Activation tagging

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   May 24, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAureus-TnCAM

   Plasmid

   #199807

   Purpose

   vector for transposome-mediated saturation mutagenesis and Tn-Seq in S. aureus

   Depositor

   Steve Salipante

   Article

   Lo et al Infect Immun. 2023 Oct 17;91(10):e002282

   Insert

   transposon cassette
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pKIR1.0

    Plasmid

    #86409

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    AtRPS5A

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. MDC1 KO sgRNA

    Plasmid

    #207103

    Purpose

    pX330 based plasmid for expression of Cas9 and the GGTGTAACGTGGAGCCAGTA sgRNA to target the MDC1 coding sequence.

    Depositor

    Jens Schmidt

    Article

    Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

    Insert

    GGTGTAACGTGGAGCCAGTA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and U6

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pETNb-cALFA

    Plasmid

    #159989

    Purpose

    For the protein expression with ALFA tag at C-terminus in E. coli

    Depositor

    Motoyuki Hattori

    Article

    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

    Type

    Empty backbone

    Use

    Tags

    HRV3C protease cleavage site-ALFA tag-8histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pHLUM (version 2)

    Plasmid

    #64166

    Purpose

    harbours marker genes HIS3, LEU2, URA3 and MET17

    Depositor

    Markus Ralser

    Article

    Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Sept. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. mKate1.31-Lifeact-7

    Plasmid

    #54668

    Purpose

    Localization: Actin, Excitation: 588, Emission: 635

    Depositor

    Michael Davidson

    Article

    Shcherbo et al Biochem J. 2009 Mar 15;418(3):567-74. do

    Type

    Empty backbone

    Use

    Tags

    LifeAct-mKate1.31

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pRSII42H-TDH3pr-mNeon

    Plasmid

    #194539

    Purpose

    High copy vector backbone with hygromycin B selectivity, encodes for TDH3(pr) driven expression of mNeon

    Depositor

    Patrick Gibney

    Article

    Smith et al Appl Microbiol. 2024; 4(1):453-469. doi:

    Insert

    hphMX
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    TDH3

    Available since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SauriBE4max

    Plasmid

    #135969

    Purpose

    Expresses AncBE4max and nSauriCas9

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLv-CMV-MyoD-ER(T)

    Plasmid

    #26809

    Depositor

    Jeffrey Chamberlain

    Article

    Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

    Insert

    MyoD-ER(T) (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 12, 2011

    Availability

    Academic Institutions and Nonprofits only

18. human K19

    Plasmid

    #14744

    Depositor

    M Bishr Omary

    Article

    Zhou et al J Biol Chem. 1999 Apr 30. 274(18):12861-

    Insert

    Keratin 19 (KRT19 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 6, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMOS011: superecliptic pHluorin (mitochondrial)

    Plasmid

    #163050

    Purpose

    superecliptic pHluorin (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    superecliptic pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRRL-VinculinTS

    Plasmid

    #111830

    Purpose

    Vinculin tension sensor in lentiviral expression vector

    Depositor

    Brenton Hoffman

    Article

    Rothenberg et al Biophys J. 2018 Apr 10;114(7):1680-1694.

    Insert

    VinculinTS (VCL Chicken)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pYPQ167

    Plasmid

    #69309

    Purpose

    Gateway entry vector with Cas9p

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    Cas9p (Plant codon-optimized; high GC content at 5 prime region)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    NLS fusion

    Promoter

    Available since

    Sept. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCN34e-ktrA-yfp

    Plasmid

    #227640

    Purpose

    c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    ktrA-yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LZF40: hSyn-Cre

    Plasmid

    #104124

    Purpose

    synOptopatch construct

    Depositor

    Adam Cohen

    Article

    Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

    Insert

    Cre
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin I

    Available since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pcDNA3/Myc-DNMT3L

    Plasmid

    #35523

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3L (DNMT3L Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AAV-oChIEF-citrine

    Plasmid

    #50974

    Purpose

    AAV transfer vector with mammalian codon-optimized ChIEF-citrine between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-citrine
    

    Use

    AAV

    Tags

    citrine

    Expression

    Mutation

    ChIEF gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCN38-yfp

    Plasmid

    #227637

    Purpose

    Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

    Depositor

    Angelika Grundling

    Article

    Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

    Insert

    yfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. MAC_C_ROS1

    Plasmid

    #187766

    Purpose

    MAC-tagged gene expression of human ROS1

    Depositor

    Markku Varjosalo

    Article

    Salokas et al EMBO Rep. 2022 Apr 5:e54041. doi: 10.152

    Insert

    ROS1 (ROS1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. VN-gamma Synuclein

    Plasmid

    #89733

    Purpose

    expresses human gamma Synuclein fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Eckermann et al Biochim Biophys Acta. 2015 Aug;1852(8):1

    Insert

    SNCG (SNCG Human)
    

    Use

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pRN112

    Plasmid

    #84464

    Purpose

    pJB38-NWMN29-30+ SarA_P1-mAmetrine-Term

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    SarA_P1-mAmetrine from pRN12
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRN10

    Plasmid

    #84454

    Purpose

    shuttle plasmid for sarA P1-mKate expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    mKate2
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pFN18A-HaloTag-SpyCatcher

    Plasmid

    #206041

    Purpose

    HaloTag-Spy0128-(Protein of Interest)-Spy0128-SpyCatcher-8xHisTag

    Depositor

    Sergi Garcia-Manyes

    Article

    Tapia-Rojo et al Nat Protoc. 2024 Mar 11. doi: 10.1038/s4

    Type

    Empty backbone

    Use

    Tags

    HaloTag and SpyCatcher-8His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMOS017: ratiometric pHluorin (cytosolic)

    Plasmid

    #163052

    Purpose

    ratiometric pHluorin (cytosolic)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    ratiometric pHluorin
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. AAV-oChIEF-tdTomato

    Plasmid

    #50977

    Purpose

    AAV transfer vector with mammalian codon-optimized ChIEF-tdTomato between AAV2 ITR

    Depositor

    Roger Tsien

    Article

    Lin et al Biophys J. 2009 Mar 4;96(5):1803-14. doi

    Insert

    ChIEF-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mutation

    ChIEF gene is mammalian codon optimized

    Promoter

    human synapsin promoter

    Available since

    Feb. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGWB2-CYCD3;1

    Plasmid

    #140750

    Purpose

    Inducing plant cells to enter mitosis

    Depositor

    Bo Liu

    Article

    Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

    Insert

    Cyclin D3;1 (CYCD3;1 Mustard Weed)
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    35S

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBAD/HisB-PAmKate

    Plasmid

    #32691

    Depositor

    Vladislav Verkhusha

    Article

    Gunewardene et al Biophys J. 2011 Sep 21;101(6):1522-8. Ep

    Insert

    PAmKate
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    F84W/S148N/S165G compared to mKate (but numbering…

    Promoter

    araB

    Available since

    Oct. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. SauriABEmax

    Plasmid

    #135968

    Purpose

    Expresses ABEmax and nSauriCas9

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pNM262

    Plasmid

    #128412

    Purpose

    Vanillic acid inducible production of lysostaphin. LsyT with the amyE signaling peptide for secretion.

    Depositor

    Christopher Voigt

    Article

    Gonzalez et al Nat Chem Biol. 2019 Dec 2. pii: 10.1038/

    Insert

    Lysostaphin (epr Staphylococcus simulans)
    

    Use

    Synthetic Biology

    Tags

    amyE SP

    Expression

    Bacterial

    Mutation

    Promoter

    Pspank(V)

    Available since

    Sept. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRN12

    Plasmid

    #84456

    Purpose

    shuttle plasmid for sarA P1-mAmetrine expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    mAmetrine
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGWB660-CYCD3;1

    Plasmid

    #140751

    Purpose

    Inducing plant cells to enter mitosis

    Depositor

    Bo Liu

    Article

    Xu et al New Phytol. 2019 Nov 2. doi: 10.1111/nph

    Insert

    Cyclin D3;1 (CYCD3;1 Mustard Weed)
    

    Use

    Tags

    TagRFP

    Expression

    Plant

    Mutation

    Promoter

    CaMV 35 promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pBPGAL4.2::p65Uw

    Plasmid

    #26229

    Depositor

    Gerald Rubin

    Article

    Pfeiffer et al Genetics. 2010 Aug 9. ():.

    Insert

    GAL4::p65 (RELA Human, Budding Yeast)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 22, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge