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PCV-Cas9

Plasmid

#123643

Purpose

Expresses Cas9 with an N-terminal HUH-tag called PCV2

Depositor

Wendy Gordon

Publication

Aird et al Commun Biol. 2018 May 31;1:54. doi: 10.1

Inserts

PCV2 (ORF1 )
Cas9

Tags

(EAAAK)8 linker and His6 + SUMO

Expression

Bacterial

Mutation

2-116

Available since

March 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLH-sgRNA1-2XPP7

Plasmid

#75390

Purpose

sgRNA1-2XPP7

Depositor

Thoru Pederson

Publication

Ma et al Nat Biotechnol. 2016 Apr 18. doi: 10.103

Insert

sgRNA1-2XPP7

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Promoter

U6

Available since

May 25, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pMaRSC

Plasmid

#89189

Purpose

The pMaRS plasmid is the same construct as pMaRSL274G, but contains a T2A-Mcherry sequence appended to the C-terminal of L274GMmMetRS.

Depositor

David Tirrell

Publication

Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Flag-L274G-T2A-mCherry (Mars1 Mouse)

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pRN1120

Plasmid

#101750

Purpose

Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

Depositor

Rene Verwaal

Publication

Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

Type

Empty backbone

Expression

Yeast

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTXB1-nbMmIgG1-Tn5

Plasmid

#184287

Purpose

Anti-mouse IgG1 Fc specific nanobody-Tn5 for NTT-seq (multiplexed CUT&Tag)

Depositor

New York Genome Center, Ivan Raimondi

Publication

Stuart et al Nat Biotechnol. 2022 Dec 19. doi: 10.103

Insert

nbMmIgG1-Tn5

Available since

May 20, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCSII-EF-miRFP709-hCdt(1/100)

Plasmid

#80007

Purpose

hCdt(1/100) fused with near-infrared fluorescent protein miRFP709 (G1 phase cell cycle reporter)

Depositor

Vladislav Verkhusha

Publication

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

hCdt1(1/100) (CDT1 Human)

Use

Lentiviral

Tags

miRFP709

Promoter

EF-1 alpha

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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mCherry- SEC61B

Plasmid

#121160

Purpose

Mammalian expression of mCherry tagged SEC61B, used as a general ER marker

Depositor

Christine Mayr

Publication

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SEC61B (SEC61B )

Expression

Mammalian

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pAAV hSyn FLExFRT mGFP-2A-Synaptophysin-mRuby

Plasmid

#71761

Purpose

Expression of membrane-tethered EGFP and Synaptophysin-mRuby from Flp-expressing neurons

Depositor

Liqun Luo

Publication

Beier et al Cell. 2015 Jul 30;162(3):622-34. doi: 10

Inserts

EGFP
Synaptophysin

Use

AAV

Tags

Palmitoylation signal and mRuby

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSR43.6r

Plasmid

#63197

Purpose

Expresses CcaS, Ho1, and PcyA constitutively

Depositor

Jeffrey Tabor

Publication

Schmidl et al ACS Synth Biol. 2014 Nov 21;3(11):820-31

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Expression

Bacterial

Available since

April 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pJK02

Plasmid

#105133

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid traJ oriT

Depositor

Joseph Sorg

Publication

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Available since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMitoLOC

Plasmid

#58980

Purpose

Expression of preCOX4-mCherry & preSU9-GFP for colocalisation analysis of yeast mitochondrila

Depositor

Markus Ralser

Publication

Vowinckel et al Mitochondrion. 2015 Sep;24:77-86. doi: 1

Inserts

mCherry
GFP

Tags

preCOX4 and preSU9

Expression

Yeast

Promoter

ADH1 and MET17

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pYPQ150

Plasmid

#69301

Purpose

Gateway entry vector with pcoCas9 (Plant codon optimized)

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

pcoCas9 (plant codon-optimized)

Use

CRISPR

Tags

3XFLAG

Mutation

NLS fusion

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pY026

Plasmid

#84741

Purpose

Expresses huAsCpf1 and crRNA guide

Depositor

Feng Zhang

Publication

Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

huAsCpf1

Tags

3xHA and NLS

Expression

Mammalian

Promoter

CMV

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET-His6-ERK2-MEK1_R4F_coexpression

Plasmid

#39212

Depositor

Melanie Cobb

Publication

Khokhlatchev et al J Biol Chem. 1997 Apr 25;272(17):11057-6

Inserts

Mek1 R4F (MAP2K1 Human)
Erk2 (Mapk1 Rat)

Tags

His6 and RBS

Expression

Bacterial

Mutation

MEK1(R4F) = S218E, S222D and deletion of AA32-51

Promoter

T7 and T7 via RBS

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

9 Articles

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pcDNA3.1-3xFLAG-TEV-UBE2O

Plasmid

#105718

Purpose

Expresses WT full length human N- terminally tagged UBE2O

Depositor

Ramanujan Hegde

Publication

Yanagitani et al Science. 2017 Aug 4;357(6350):472-475. d

Insert

UBE2O (UBE2O Human)

Tags

3xFLAG-TEV

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLL3.7 FLAG-YAP1-TEAD-P-H2B-mCherry

Plasmid

#128327

Purpose

Reporter to evaluate YAP1/TEAD-mediated gene transcription

Depositor

Yutaka Hata

Publication

Maruyama et al Mol Cancer Res. 2018 Feb;16(2):197-211.

Inserts

H2B mCherry
YAP1 (YAP1 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-calpainsensor

Plasmid

#36182

Purpose

detection of activity of ubiquitous calpains

Depositor

Isabelle Richard

Publication

Stockholm et al J Mol Biol. 2005 Feb 11;346(1):215-22. E

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

alpha-Fodrin cleavage site (Sptan1 Mouse)

Tags

eCFP and eYFP

Expression

Mammalian

Promoter

CMV

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-nEF Con/Fon hChR2(H134R)-EYFP

Plasmid

#55644

Purpose

Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Publication

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Promoter

nEF

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pICH41780

Plasmid

#48019

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

12 Articles

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pHes1(2.5k)-luc

Plasmid

#43806

Depositor

Ryoichiro Kageyama

Publication

Takebayashi et al J Biol Chem. 1994 Feb 18;269(7):5150-6.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Hes1 Promoter (Hes1 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Mutation

Contains the murine Hes1 promoter

Promoter

Hes1 Promoter

Available since

March 20, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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