We narrowed to 55,043 results for: tra

1. pSimpleII-U6-tracr-U6-crRNA(tdTomato)-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47869

   Purpose

   All-in-one plasmid targeting tdTomato

   Depositor

   James Thomson, Erik Sontheimer

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Inserts

   NmCas9
   U6pr-tracrRNA
   tdTomato crRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Available Since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCyPet-FHA2-AuroraB Substrate-YPet-IRES-puro2b

   Plasmid

   #40731

   Depositor

   Daniel Gerlich

   Article

   Wurzenberger et al J Cell Biol. 2012 Jul 23;198(2):173-83.

   Insert

   Aurora B FRET-sensor
   

   Tags

   CyPet and YPet

   Expression

   Bacterial and Mammalian

   Promoter

   CMV

   Available Since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSimpleII-U6-tracr-U6-crRNA(OCT4)-NLS-NmCas9-HA-NLS(s)

   Plasmid

   #47870

   Purpose

   All-in-one plasmid targeting human OCT4, cuts roughly ~84bp downstream of stop codon.

   Depositor

   James Thomson, Erik Sontheimer

   Article

   Hou et al Proc Natl Acad Sci U S A. 2013 Aug 12.

   Inserts

   NmCas9
   U6pr-tracrRNA
   OCT4 crRNA
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Available Since

   Sept. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET His6 TEV co-transformation cloning vector (13S-B)

   Plasmid

   #48324

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET His6 TEV co-transformation cloning vector (13K-B)

   Plasmid

   #48316

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   His6

   Expression

   Bacterial

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pspCas9-ABE-3'UTR-sgRNA-Tetra-com-vector

   Plasmid

   #132560

   Purpose

   Vector plasmid expressing ABEmax and sgRNA scaffold with com replacing the Tetraloop.

   Depositor

   Baisong Lu

   Article

   Lyu et al CRISPR J. 2021 Feb;4(1):69-81. doi: 10.1

   Inserts

   ABEmax
   sgRNA, with Tetraloop replaced by com aptamer
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

   Plasmid

   #157985

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET StrepII TEV co-transformation cloning vector (13S-R)

   Plasmid

   #48328

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   StrepII

   Expression

   Bacterial

   Available Since

   Sept. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. PX398 Nitratifractor salsuginis str DSM 16511 Cas9

   Plasmid

   #68334

   Purpose

   Nitratifractor salsuginis str DSM 16511 Cas9

   Depositor

   Feng Zhang

   Insert

   NsCas9
   

   Use

   CRISPR

   Tags

   HA and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET StrepII TEV co-transformation cloning vector (13K-R)

    Plasmid

    #48319

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    StrepII

    Expression

    Bacterial

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1 Flag YFP hNKCC1 extracellular-cysteineless (NT864)

    Plasmid

    #49069

    Purpose

    Expresses human NKCC1 mutant lacking extracellular cysteine residues and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C563S,C568S,C577S,C582S in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Nov. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. Human Coxsackievirus A16 strain G10 inactive 2A protease

    Plasmid

    #228665

    Purpose

    Bacterial expression of the A71 2A protease, innactive and can be used as a control in assays

    Depositor

    ASAP Discovery, Frank von Delft

    Insert

    Enterovirus A71 2A protease
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Mutation

    Avitag insertion and C2A mutations / Active site …

    Available Since

    Feb. 18, 2025

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pcDNA3.1-SLC25A46[R257A,R347A]-P2A-Electra1 (pAH36)

    Plasmid

    #227620

    Purpose

    Overexpression of SLC25A46 with R247A and R347A mutations in mammalian cell lines

    Depositor

    Luke Chao

    Article

    Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

    Insert

    SLC25A46 (SLC25A46 Human)
    

    Tags

    P2A-mElectra1

    Expression

    Mammalian

    Mutation

    R257A,R347A

    Promoter

    CMV, T7

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR

    Plasmid

    #211815

    Purpose

    lentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishment

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    SpCas9, tracrRNA and puromycin selection gene
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    U1a promoter, U6 promoter, and hPGK promoter

    Available Since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUPD2 sgRNA:scaffold tetraloop MS2 aptamer SAM (GB1436)

    Plasmid

    #160570

    Purpose

    A version of scaffold sgRNA with the sequence of MS2 aptamer inside the tetraloop of the scaffold

    Depositor

    Diego Orzaez

    Article

    Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

    Insert

    sgRNA:scaffold tetraloop MS2 aptamer SAM
    

    Use

    CRISPR

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    Dec. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 Flag YFP hNKCC1 intracellular-cysteineless (NT855)

    Plasmid

    #49080

    Purpose

    Expresses human NKCC1 mutant lacking cysteine residues within intracellular loops and with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Article

    Somasekharan et al PLoS One. 2013 Dec 5;8(12):e82060. doi:

    Insert

    human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
    

    Tags

    3x Flag and mVenus

    Expression

    Mammalian

    Mutation

    C791S, C820S, C1052S in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Nov. 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET His6 Thioredoxin TEV co-transformation cloning vector (13K-T)

    Plasmid

    #48322

    Depositor

    Scott Gradia

    Type

    Empty backbone

    Tags

    His6 and Thioredoxin

    Expression

    Bacterial

    Available Since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. NYESO beta/alpha into TRAC HDRT Source (pTR 169)

    Plasmid

    #112021

    Purpose

    DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha

    Depositor

    Alexander Marson

    Article

    Roth et al Nature. 2018 Jul 11. pii: 10.1038/s41586

    Insert

    NYESO beta/alpha into TRAC HDRT
    

    Use

    CRISPR and Synthetic Biology

    Available Since

    Sept. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pX260-U6-DR-BB-DR-Cbh-NLS-hSpCas9-NLS-H1-shorttracr-PGK-puro

    Plasmid

    #42229

    Purpose

    This plasmid separately encodes a human codon-optimized SpCas9, a tracrRNA and customizable crRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLV-Ftractin-mRuby3-p2A-mTurquoise-MLC-IRES-Blast

    Plasmid

    #85146

    Purpose

    lentiviral expression of a stoichiometric F-actin and myosin light chain reporter

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Insert

    Ftractin-mRuby3-P2A-mTurquoise-MLC
    

    Use

    Lentiviral

    Tags

    mRuby3, mTurquoise

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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