We narrowed to 19,994 results for: IRE

Showing: 8581 - 8600 of 19994 results

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pAAV-hSyn1-EGFP-P2A-EGFPf-WPRE-HGHpA

Plasmid

#74513

Purpose

AAV vector that use human synapsin-1 promoter to drive the expression of EGFP and membrane-targeted EGFPf linked by self-cleaving P2A peptide.

Depositor

Guoping Feng

Article

Zhang et al J Neurosci. 2016 Feb 17;36(7):2247-60. d

Insert

EGFP-p2A-EGFP-f

Use

AAV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-SpCas9

Plasmid

#104588

Purpose

AAV vector expressing Myc-tagged SpCas9 under the EFS promoter

Depositor

Ryohei Yasuda

Article

Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

Has Service

AAV8

Insert

Streptococcus pyogenes Cas9

Use

AAV and CRISPR

Tags

Myc

Expression

Mammalian

Promoter

EFS

Available Since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-ADAM17-HA

Plasmid

#65105

Purpose

Expression of HA tagged ADAM17 (TACE) in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

ADAM17 (ADAM17 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR_zeo_sgNT135

Plasmid

#183181

Purpose

pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

NT135

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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LCC-ICCG_pET21b

Plasmid

#215408

Purpose

Expression construct for LCC-ICCG gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

LCC-ICCG

Expression

Bacterial

Mutation

F243I/D238C/S283C/Y127G

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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SPLICS PM-ER Short P2A

Plasmid

#164112

Purpose

Detect the short Plasma membrane-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP PM-ER Short P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV6-AC EJ7-GFP

Plasmid

#113617

Purpose

Reporter for canonical-NHEJ. Reporter for end joining between two double-strand breaks, induced with the 7a and 7b sgRNAs with CAS9. EJ between the distal ends w/o indel mutations restores GFP

Depositor

Jeremy Stark

Article

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

EJ7-GFP variant of EGFP

Expression

Mammalian

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-16

Plasmid

#172746

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-16; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-16

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Promoter

T7

Available Since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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GST-p25

Plasmid

#24896

Depositor

Harish Pant

Article

Amin et al J Neurosci Res. 2002 Feb 1. 67(3):354-62

Insert

p25 (CDK5R1 Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal truncated version of p35, contains aa …

Available Since

May 17, 2010

Availability

Academic Institutions and Nonprofits only
SPLICS PM-ER Long P2A

Plasmid

#164111

Purpose

Detect the long Plasma membrane-Endoplasmic reticulum contact

Depositor

Marisa Brini, Tito Calì

Article

Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

Insert

Split YFP PM-ER Long P2A

Expression

Mammalian

Available Since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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WT (REP10)

Plasmid

#21368

Depositor

Gregory Germino

Article

Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Expression

Mammalian

Available Since

Jan. 4, 2010

Availability

Academic Institutions and Nonprofits only

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pXR001: EF1a-CasRx-2A-EGFP

Plasmid

#109049

Purpose

CasRx (NLS-RfxCas13d-NLS) with 2A-EGFP for RNA targeting

Depositor

Patrick Hsu

Article

Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

Insert

CasRx

Use

CRISPR and Lentiviral

Tags

2A-eGFP, HA, and NLS

Expression

Mammalian

Promoter

EF1A

Available Since

May 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-BFP

Plasmid

#78545

Purpose

Fusion protein Cas9+BFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

BFP

Use

Lentiviral

Expression

Mammalian

Promoter

Fusion protein with Cas9

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pLentiCas9-T2A-BFP

Plasmid

#78547

Purpose

Cas9 protein with BFP

Depositor

Roderic Guigo, Rory Johnson

Article

Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

Insert

T2A-BFP

Use

Lentiviral

Expression

Mammalian

Promoter

In frame with Cas9

Available Since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_NDG

Plasmid

#26820

Depositor

Derrick Rossi

Article

Warren et al Cell Stem Cell. 2010 Nov 5;7(5):618-30.

Insert

5'UTR-NDGFP-3'UTR

Expression

Mammalian

Mutation

nuclear destabilized eGFP

Available Since

Nov. 17, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pXD70LacZ6-LacI-Ptac(LacO)-LacZ

Plasmid

#191621

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), IPTG-inducible promoter-lacZ, engineered from pMAL-c2X

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

lacZ

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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ABEx1_SpRY_V106W (ABE8e_V28C_nCas9_SpRY_V106W)

Plasmid

#251552

Purpose

Mammalian expression plasmid encoding an nCas9-SpRY_V106W adenine base editor (ABE8e) carrying the V28C mutation in the deaminase domain

Depositor

George Church

Article

Perrotta et al Nat Biotechnol. 2025 Dec 18. doi: 10.103

Insert

ecTadA(8e)_V28C-nCas9_SpRY_V106W

Use

CRISPR

Expression

Mammalian

Mutation

ABEx4 V28C_M151E

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG-LKB1

Plasmid

#8590

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Tags

FLAG

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pNluc-hbb

Plasmid

#239753

Purpose

Template for in vitro transcription of nanoluciferase flanked by the human haemoglobin beta 5' and 3' UTRs.

Depositor

Catherine Jopling

Article

Smart et al Mol Ther Nucleic Acids. 2025 Jun 24;36(3

Insert

Nanoluciferase flanked by human haemoglobin beta 5' and 3' UTRs

Use

Luciferase

Promoter

T7 (included as part of insert)

Available Since

July 7, 2025

Availability

Academic Institutions and Nonprofits only

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AZ64_pE.DonorCLYBL.TS

Plasmid

#199228

Purpose

Donor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locus

Depositor

Manuel Goncalves

Article

Wang et al Nucleic Acids Res. 2023 Mar 17:gkad165.

Insert

Expression unit for EGFP reporter

Use

Gene targeting donor plasmid

Expression

Mammalian

Promoter

Human PGK1 gene promoter

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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