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pORANGE Tubb3-GFP KI

Plasmid

#131497

Purpose

Endogenous tagging of β3 Tubulin: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Publication

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Tubb3, Tubb3 Rat, Mouse)

Expression

Mammalian

Promoter

U6 and Cbh

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-C-terminal GST

Plasmid

#128025

Purpose

Mammalian expression vector with C terminal GST tag

Depositor

Oskar Laur

Type

Empty backbone

Tags

GST

Expression

Mammalian

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-iGlucoSnFR-TS

Plasmid

#127952

Purpose

Expresses the iGlucoSnFR-TS biosensor for intracellular [glucose], with a fluorescence-lifetime readout, in mammalian cells

Depositor

Gary Yellen

Publication

Diaz-Garcia et al J Neurosci Res. 2019 May 20. doi: 10.100

Insert

iGlucoSnFR-TS

Use

AAV

Tags

His6 and Xpress epitope

Expression

Mammalian

Promoter

CAG

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLenti-PGK-Neo-PIP-NLS-mVenus

Plasmid

#118619

Purpose

Lentiviral expression of the cell cycle marker PIP-NLS-mVenus

Depositor

Jean Cook

Publication

Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

Insert

PIP-NLS-mVenus

Use

Lentiviral

Tags

mVenus

Expression

Mammalian

Promoter

hPGK

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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GAG-CRErec

Plasmid

#119971

Purpose

Expresses GAG (FMLV) fused with CRE recombinase for the production of VLPs loaded with CRE protein

Depositor

Philippe Mangeot, Théophile Ohlmann, Emiliano Ricci

Publication

Mangeot et al Nat Commun. 2019 Jan 3;10(1):45. doi: 10

Insert

GAG-nlsCRErec

Tags

nls

Expression

Mammalian

Promoter

hCMV

Available since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pET21a-CysRS-His

Plasmid

#124107

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Publication

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Cysteinyl-tRNA ligase (cysS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBLO 62.5

Plasmid

#123124

Purpose

Human expression vector for planctomyces CasX and sgRNA

Depositor

Jennifer Doudna, Benjamin Oakes

Publication

Liu et al Nature. 2019 Feb 4. pii: 10.1038/s41586-

Inserts

CasX protein
CasX sgRNA

Use

CRISPR

Expression

Mammalian

Available since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pcDNA3.1_MMP-9-HA

Plasmid

#121172

Purpose

Expression of mouse MMP9

Depositor

M. Elizabeth Fini, Shinwu Jeong

Publication

Jeong et al Am J Pathol. 2012 May;180(5):2028-39. do

Insert

MMP9 (matrix metallopeptidase 9) (Mmp9 Mouse)

Tags

HA

Expression

Mammalian

Mutation

See depositor comments below

Promoter

CMV immediate/early

Available since

March 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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HIV-1 LTR-gfp

Plasmid

#115809

Purpose

HIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr

Depositor

Jeremy Luban

Publication

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

gfp and ngfr

Use

Lentiviral

Promoter

HIV1 LTR

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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EFS-GFP

Plasmid

#110834

Purpose

positive control for GFP expression

Depositor

Michael Lewis

Publication

Wei et al Stem Cells. 2014 Oct;32(10):2571-82. doi

Insert

elongation factor 1alpha binding sequence (EFS) at GFP promoter region

Use

Lentiviral

Expression

Mammalian

Available since

May 25, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pLJM1 EGFP-Tau

Plasmid

#108868

Purpose

Expresses EGFP linked to human Tau

Depositor

Gabriele Kaminski Schierle

Insert

Tau (MAPT Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

human four-repeat tau lacking the N-terminal sequ…

Promoter

CMV

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pENN.AAV.tMCK.PI.eGFP.WPRE.bGH

Plasmid

#105556

Purpose

AAV expression of EGFP from tMCK promoter

Depositor

James M. Wilson

Has service

AAV9

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

tMCK

Available since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pZyxin-mMaple3

Plasmid

#101151

Purpose

Fluorescent protein for superresolution imaging and a focal adhesion protein for binding substrate

Depositor

Xiaowei Zhuang

Publication

Wang et al Proc Natl Acad Sci U S A. 2014 Jun 10;11

Insert

human codon optimized mMaple3 fused to zyxin

Expression

Mammalian

Promoter

cmv

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV_hsyn_NES-his-CaMPARI2-L398T-WPRE-SV40

Plasmid

#101064

Purpose

AAV vector expressing CaMPARI2_L398T (Kd = 825nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

Depositor

Eric Schreiter

Publication

Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

Has service

AAV1

Insert

CaMPARI2_L398T

Use

AAV

Tags

FLAG-HA-myc and NES_his

Promoter

hsyn (synapsin-1)

Available since

Oct. 2, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-CAG-tTA

Plasmid

#99118

Purpose

An AAV genome that ubiquitously expresses tTA from the CAG promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has service

AAV2

Insert

tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

CAG

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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AID-BE3

Plasmid

#100803

Purpose

Expresses AID-BE3 in mammalian cells

Depositor

David Liu

Publication

Komor et al Sci Adv. 2017 Aug 30;3(8):eaao4774. doi:

Insert

activation induced cytidine deaminase (AICDA S. pyogenes, Human)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 31, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-CAG-mRuby2

Plasmid

#99123

Purpose

An AAV genome that ubiquitously expresses the fluorescent protein mRuby2 from the CAG promoter

Depositor

Viviana Gradinaru

Publication

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Insert

mRuby2

Use

AAV

Expression

Mammalian

Promoter

CAG

Available since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pDIRECT_22A

Plasmid

#91133

Purpose

Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9 + AtU6:gRNA, Plant Selection: 2x35S:npt II

Depositor

Daniel Voytas

Publication

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

Engineering Reagent: 35S:AtCas9 + AtU6:gRNA

Use

CRISPR

Expression

Plant

Available since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pEFIRES-P-mCherry-HPos

Plasmid

#87159

Purpose

Fluorescent marker of lipid droplets (LDs)

Depositor

Elina Ikonen

Publication

Salo et al EMBO J. 2016 Dec 15;35(24):2699-2716. Ep

Insert

HPos

Tags

mCherry

Expression

Mammalian

Promoter

EF-1-alpha

Available since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDule-tfmF A65V S158A

Plasmid

#85484

Purpose

Machinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNA

Depositor

Ryan Mehl

Publication

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

tri-fluoromethyl-phenylalanine Mj synthetase A65V S158A

Tags

None

Expression

Bacterial

Mutation

Y32V F108W Q109M I159M with 65Val and 158Ala

Promoter

lpp (constitutive)

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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