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pET-45b-mEGFP-HMGB1-WT

Plasmid

#194543

Purpose

Bacterial Expression of 6xHis mEGFP-HMGB1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB1 (HMGB1 Human)

Tags

mEGFP

Expression

Bacterial

Available Since

Feb. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pX458-DsRed2

Plasmid

#112219

Purpose

Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2

Depositor

Yinming Liang

Article

Luo et al J Biotechnol. 2018 Jun 7;281:11-20. doi:

Insert

DsRed2

Use

CRISPR

Expression

Mammalian

Mutation

DsRed2 contains a silent nucleotide substitution …

Available Since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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hRad51(A89E)–Cas9(D10A)

Plasmid

#125563

Purpose

Expresses the Rad51(A89E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(A89E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCHC039 (∆phcA)

Plasmid

#226207

Purpose

Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

Depositor

Christopher Johnson

Article

Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

Insert

Homology arms for PhcA

Use

Synthetic Biology

Available Since

Nov. 18, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCW57.1-4EBP1_4xAla

Plasmid

#38240

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

4EBP1 (Eif4ebp1 hybrid gene of rat and human, Rat)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Mutation

T37A, T46A, S65A, T70A (dominant negative)

Promoter

Tet ON

Available Since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pPerimCh

Plasmid

#227013

Purpose

Expresses periplasmic mCherry

Depositor

Suzan Rooijakkers

Article

Heesterbeek et al Sci Rep. 2019 Feb 28;9(1):3074. doi: 10.

Insert

PelB-mCherry, arabinose inducible cytosolic GFP

Expression

Bacterial

Promoter

rpsM and pBAD

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CBh-mKate2-IRES-MCS (WT-WT)

Plasmid

#105921

Purpose

AAV-mediated gene expression from CBh promoter. Contains canonical left and right AAV2 ITRs

Depositor

Marcella Patrick

Has Service

AAV2

Inserts

mKate2
IRES-Multiple cloning site

Use

AAV

Mutation

codon optimized

Promoter

CBh and same CBh promoter as mKate2

Available Since

Feb. 15, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pLH01

Plasmid

#117261

Purpose

Genome editing for Lactobacillus plantarum WCSF1

Depositor

Sheng Yang

Article

Huang et al Biotechnol J. 2019 Jul;14(7):e1800690. d

Insert

PorfX-Lp_0640-42, sapK-sappR

Use

Other

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo

Plasmid

#108101

Purpose

Lentiviral expression plasmid of cDNA with neomycin resistance gene (empty vector)

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

FLAG tag

Expression

Mammalian

Promoter

EFS promoter

Available Since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP4K4

Plasmid

#23486

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP4K4 (MAP4K4 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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gRNA_GFP-T1

Plasmid

#41819

Purpose

Expresses a guide RNA (gRNA) to target GFP (T1 target sequence) for genome engineering

Depositor

George Church

Article

Mali et al Science. 2013 Feb 15;339(6121):823-6. do

Insert

gRNA_GFP-T1

Use

CRISPR

Expression

Mammalian

Available Since

Jan. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pMpGWB313

Plasmid

#68641

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

MpEF1alpha promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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mAmetrine-C1

Plasmid

#54660

Purpose

Localization: C1 Cloning Vector, Excitation: 406, Emission: 526

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al Nat Methods. 2008 May;5(5):401-3. doi: 1

Type

Empty backbone

Tags

mAmetrine

Expression

Mammalian

Available Since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pSM.wrmScarlet1-10::SL2::GFP1-10

Plasmid

#234318

Purpose

For the expression of wrmScarlet1-10 and GFP1-10 in C. elegans

Depositor

Kota Mizumoto

Article

Kurashina et al Genetics. 2024 Dec 22:iyae214. doi: 10.1

Insert

wrmScarlet1-10::SL2::GFP1-10

Expression

Worm

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pLminP_Luc2P_RE56

Plasmid

#247034

Purpose

Glucocorticoid Receptor pathway reporter; lentiviral expression of NR3C1 (GR) DNA response element cloned 5' to firefly luciferase. Coexpresses EmGFP to monitor lentiviral transduction.

Depositor

Ramnik Xavier

Article

O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

Insert

Firefly luciferase

Use

Lentiviral

Promoter

NR3C1 (GR)

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pKLBac-FluB-trimer

Plasmid

#229859

Purpose

Influenza B/Memphis/13/03 polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Reich et al Nature. 2014 Dec 18;516(7531):361-6. doi

Inserts

PA, Influenza B (B/Memphis/13/03)
PB1, Influenza B (B/Memphis/13/03)
PB2, Influenza B (B/Los Angeles/1/02)

Tags

2x-Streptag and 8xHis-tag

Expression

Insect

Available Since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBAD/His-miRFP670

Plasmid

#79984

Purpose

Monomeric near-infrared fluorescent protein miRFP670 in bacterial plasmid

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Commun. 2016 Aug 19;7:12405. doi: 10

Insert

miRFP670

Expression

Bacterial

Promoter

araB

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR5'_ubi

Plasmid

#27320

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

zebrafish ubiquitin promoter (ubb Zebrafish)

Use

Multisite gateway 5' entry vector

Available Since

Feb. 3, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA-f:PGC1

Plasmid

#1026

Depositor

Bruce Spiegelman

Article

Monsalve et al Mol Cell 2000 Aug;6(2):307-16.

Insert

PPAR gamma coactivator 1 alpha (Ppargc1a Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-Tol2

Plasmid

#31823

Depositor

Stephen Ekker

Article

Balciunas et al PLoS Genet. 2006 Nov 10;2(11):e169. Epub

Insert

Tol2 Transposase

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only

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