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HaloTag-TREM2 Vector

Plasmid

#238682

Purpose

Express HaloTag-TREM2 Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

TREM2 (TREM2 Human)

Tags

HaloTag (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Sept. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pGL4 [luc2P/hID1/Hygro] Vector

Plasmid

#236808

Purpose

Express hID1 response element in luciferase reporter assay

Depositor

Promega Corporation

Has Service

DNA

Insert

hID1 (C17orf28 Human)

Use

Luciferase

Mutation

None

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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AAVS1 EGFP-p62 HRD

Plasmid

#207550

Purpose

Homologous recombination donor to integrate and expression cassette for eGFP-p62 in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-P62

Tags

EGFP

Expression

Mammalian

Promoter

Endogenous

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pXJ-Myc-TCF7L2

Plasmid

#232644

Purpose

transient expresses TCF7L2 in mammalian cells

Depositor

Chen-Yang Shen

Article

Chou et al NPJ Genom Med. 2022 Jan 19;7(1):4. doi:

Insert

TCF7L2 (TCF7L2 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

March 6, 2025

Availability

Academic Institutions and Nonprofits only

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lvl0-G-CasE

Plasmid

#229184

Purpose

lvl 0 5 gene

Depositor

Ron Weiss

Article

Duportet et al Nucleic Acids Res. 2014 Dec 1;42(21):134

Insert

CasE

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-LC3B HRD

Plasmid

#207551

Purpose

Homologous recombination donor to integrate and expression cassette for eGFP-LC3B in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-LC3B

Tags

EGFP

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1 EGFP-GABARAPL1 HRD

Plasmid

#207552

Purpose

Homologous recombination donor to integrate and expression cassette for EGFP-GABRAPL1 in the AAVS1 locus of human cells

Depositor

Jens Schmidt

Article

Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

Insert

TET inducible EGFP-GABARAPL1

Tags

EGFP

Expression

Mammalian

Promoter

Endogenous

Available Since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SEMA3E-Fc(DAPA)-AviTag-6xHis

Plasmid

#156970

Purpose

Mammalian expression of secreted protein fused to Fc(DAPA)-Avi-6xHis.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SEMA3E (SEMA3E Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPC1-hVAP-A KD/MD

Plasmid

#104449

Purpose

Expression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to GFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Alpy et al J Cell Sci. 2013 Dec 1;126(Pt 23):5500-1

Insert

vesicle-associated membrane protein-associated protein A (VAPA Human)

Tags

EGFP

Expression

Mammalian

Mutation

changed K94 to D and M96 to D

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pDONR223-CRKL

Plasmid

#23354

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CRKL (CRKL Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pRK5-HA-mCherry-raptor

Plasmid

#73386

Purpose

Expresses raptor tagged with mCherry and HA

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

Regulatory Associated Protein Of MTOR, Complex 1 (RPTOR Human)

Tags

HA and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Leo1

Plasmid

#11061

Depositor

Matthew Meyerson

Article

Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

Insert

Leo1 (LEO1 Human)

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only

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pCENPTΔC-dCas9-3xGFP

Plasmid

#198325

Purpose

Expresses CENPTΔC-dCas9-3xGFP protein in mammalian cells. When coupled with a guide RNA against a high repeat target locus, this can be applied to seed ectopic kinetochores at the target locus

Depositor

Sarah McClelland

Article

Tovini et al EMBO J. 2023 May 15;42(10):e111587. doi:

Insert

CENPTΔC (CENPT Human)

Use

Lentiviral

Tags

EGFP x 3 and dCas9

Expression

Mammalian

Mutation

truncation - encodes only amino acids 1-375

Promoter

CMV-TetO

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-SLC38A5_STOP

Plasmid

#161284

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A5 (SLC38A5 Human)

Expression

Mammalian

Available Since

Sept. 15, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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-962 human cyclin D1 promoter pGL3Basic

Plasmid

#32727

Depositor

Frank McCormick

Article

Tetsu et al Nature. 1999 Apr 1;398(6726):422-6.

Insert

CCND1 promoter (CCND1 Human)

Use

Luciferase; Luciferase reporter gene

Promoter

-962 CCND1

Available Since

Jan. 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hADARB2-3xFLAG

Plasmid

#165470

Purpose

Human ADARB2 mammalian expression vector with 3 C-terminal FLAG tags.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Adenosine deaminase RNA specific B2 (inactive) (ADARB2 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGEx-4T-1-YTHDF3

Plasmid

#70088

Purpose

Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only
pHBS838 TDP43 RRM-GFP

Plasmid

#98249

Purpose

TDP43 RRM-GFP reporter construct

Depositor

Rajat Rohatgi

Article

Schmidt et al Cell Rep. 2016 Aug 2;16(5):1228-36. doi:

Insert

TARDBP (TARDBP Human)

Tags

Internal GFP to replace RRM domains

Expression

Mammalian

Promoter

CMV

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-Nup35

Plasmid

#87342

Purpose

To express human Nup35 in mammalian cells

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

nucleoporin 35 (NUP35 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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AAV-CAG::FLEX-rev:: ChR2HA-2a-hM4D

Plasmid

#52521

Purpose

Co-expresses HA tagged channelrhodopsin and hM4D (Gi) from a Cre-dependent virus

Depositor

Scott Sternson

Article

Stachniak et al Neuron. 2014 May 21;82(4):797-808. doi:

Insert

ChR2-2a-hM4D

Use

AAV and Cre/Lox

Tags

2xHA on ChR2

Promoter

CBA

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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