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pLVX-CMV-Galpha-12 ONE-GO

Plasmid

#189739

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-12 ONE-GO
GNA12 (GNA12 Human)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-RB1-shRNA63

Plasmid

#25641

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 63 (RB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pLV CMV mCherry-DDX1-V5

Plasmid

#175162

Purpose

Lentiviral expression of human DDX1-V5

Depositor

Larry Gerace

Article

Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

Insert

DDX1 (DDX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-z ONE-GO

Plasmid

#189734

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-z ONE-GO
GNAZ (Gnaz Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV-EGFP-eDHFR(69K6)-MCS

Plasmid

#172100

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFR(69K6)

Depositor

Shinya Tsukiji

Article

Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

Insert

EGFP-eDHFR(69K6)-20aa

Expression

Mammalian

Mutation

eDHFR(69K6): Hexalysine (K6) sequence is inserted…

Promoter

CMV

Available Since

July 14, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV2-SynTetOff-palGFP

Plasmid

#190232

Purpose

AAV vector, high-level expression of EGFP with a palmitoylation signal derived from GAP-43 N-terminus (palGFP) in neuronal cells

Depositor

Hiroyuki Hioki

Article

Sohn et al PLoS One. 2017 Jan 6;12(1):e0169611. doi

Insert

EGFP

Use

AAV

Tags

palmitoylation signal sequence

Expression

Mammalian

Available Since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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VN-alpha Synuclein

Plasmid

#89470

Purpose

expresses human alpha Synuclein fused to the N-terminal part of Venus

Depositor

Bradley Hyman, Tiago Outeiro

Article

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Insert

SNCA (SNCA Human)

Tags

N-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBW2287_pCAG-PV1-PYL-L1-FlpO-397C-NLS-BGHpA

Plasmid

#108770

Purpose

Expresses aa397 to C-terminus of Flp recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa397 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-hCGN

Plasmid

#236507

Purpose

For expression of Cingulin (human) in mammalian cells

Depositor

Sandra Citi

Article

Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

Insert

CGN (CGN Human)

Tags

GFP

Expression

Mammalian

Mutation

WT

Available Since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2-CRY2-mCherry

Plasmid

#140571

Purpose

For use in light-induced protein inactivation through the interaction with CIB1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Mammalian

Promoter

CMV IE94

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.4 human alpha5 full

Plasmid

#221396

Purpose

Mammalian expression of human integrin alpha5 full length

Depositor

Timothy Springer

Article

Li et al Cell. 2024 May 14:S0092-8674(24)00475-6.

Insert

integrin alpha5 full length (ITGA5 Human)

Tags

CD33 secretion peptide (MPLLLLLPLLWAGALA) and St…

Expression

Mammalian

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO-RB1-shRNA19

Plasmid

#25640

Purpose

3rd generation lentiviral vector

Depositor

Todd Waldman

Article

Michaud et al Cancer Res. 2010 Apr 15. 70(8):3228-38.

Insert

RB1-shRNA 19 (RB1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

June 21, 2010

Availability

Academic Institutions and Nonprofits only

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pDmAct5C::Cas9-2A-Neo

Plasmid

#176679

Purpose

Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)

Use

Crispr

Expression

Insect

Mutation

Drosophila codon optimized

Promoter

D. melanogaster Act5C

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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p5E--3mnx1

Plasmid

#74632

Purpose

Can be used to drive expression specifically in zebrafish motor neurons

Depositor

Nicholas Cole

Article

Don et al Zebrafish. 2016 Sep 15.

Insert

motor neuron and pancreas homeobox 1 promoter (mnx1 Zebrafish)

Use

5' entry vector for multisite gateway three …

Promoter

-3mnx1

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pLVX-CMV-Galpha-oB ONE-GO

Plasmid

#189733

Purpose

GPCR/G protein BRET biosensor

Depositor

Mikel Garcia-Marcos

Article

Janicot et al Cell. 2024 Feb 16:S0092-8674(24)00065-5.

Inserts

Galpha-oB ONE-GO
GNAO (Gnao1 Rat)

Use

Lentiviral

Available Since

Jan. 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP Abi1

Plasmid

#74905

Purpose

Expresses human Abi1 fused to GFP

Depositor

Giorgio Scita

Article

Innocenti et al Nat Cell Biol. 2004 Apr;6(4):319-27. Epu

Insert

Abi1 (ABL1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 3, 2016

Availability

Academic Institutions and Nonprofits only

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pmGFP11C-Tau

Plasmid

#71434

Purpose

Split GFP plasmid for visualization of Tau dimerization. Used together with plasmid 71433

Depositor

Henri Huttunen

Article

Brunello et al Sci Rep. 2016 Jul 27;6:30498. doi: 10.10

Insert

Tau (0N4R) (MAPT Human)

Tags

GFP (213-228)

Expression

Mammalian

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only
pGL4_NFAT-RE-CMVmin-BC0524-luc2

Plasmid

#227139

Purpose

Barcoded assay, Ca2+ sensor; barcode BC0524

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

6x clustered NFAT element linked to CMV minimal promoter driving barcode 0524 and a luciferase reporter gene

Expression

Mammalian

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLV-Kif5a-Myc

Plasmid

#186613

Purpose

Third generation lentiviral vector expressing Kinesin Heavy Chain 5 isoform A fused to Myc tag

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Kinesin Heavy Chain isoform 5A (KIF5A Human)

Use

Lentiviral

Tags

Myc tag

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG-hPPP3CB-FLAG

Plasmid

#179135

Purpose

Expression vector of human protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

Depositor

Masahito Ikawa

Article

Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

Insert

protein phosphatase 3, catalytic subunit, beta isoform (PPP3CB Human)

Tags

FLAG

Expression

Mammalian

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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