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pKLV2-EF1a-BsdCas9-W

Plasmid

#67978

Purpose

Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the N-terminus

Depositor

Kosuke Yusa

Publication

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

EF1a-Cas9 cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Cas9 fused with Flag-tag and a NLS at the N termi…

Promoter

EF1a

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

13 Articles

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pDrVSP-IRES2-EGFP

Plasmid

#80333

Purpose

Expresses Danio rerio VSP with separated EGFP in mammalian cell

Depositor

Yasushi Okamura

Publication

Hossain et al J Biol Chem. 2008 Jun 27;283(26):18248-5

Insert

Daino rerio voltage-sensing phosphatase (tpte Zebrafish)

Expression

Mammalian

Promoter

CMV

Available since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pLV-tetO-Tfap2c

Plasmid

#70269

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Tfap2c

Depositor

Hubert Schorle

Publication

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

transcription factor AP-2, gamma (Tfap2c Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pAAV-EF1a-CVS-G-WPRE-pGHpA

Plasmid

#67528

Purpose

"Expresses the rabies glycoprotein"

Depositor

Botond Roska

Publication

Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

Insert

Rabies virus strain CVS-11 glycoprotein (G)

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available since

July 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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perbB2-ECFP

Plasmid

#40268

Depositor

Martin Offterdinger

Publication

Offterdinger et al Traffic. 2008 Jan;9(1):147-55. Epub 2007

Insert

erbB2 (ERBB2 Human)

Tags

ecfp

Expression

Mammalian

Promoter

CMV

Available since

Nov. 26, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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FLAG-Matr3 delRRM2

Plasmid

#32882

Depositor

Yossi Shiloh

Publication

Salton et al PLoS One. 2011;6(8):e23882. Epub 2011 Au

Insert

MATR3 Matrin3 (MATR3 Human)

Tags

FLAG

Expression

Bacterial and Mammalian

Mutation

deleted amino acids 497-566

Promoter

CMV

Available since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
pLKO.1 p18 shRNA

Plasmid

#26631

Depositor

David Sabatini

Publication

Sancak et al Cell. 2010 Apr 16. 141(2):290-303.

Insert

p18 shRNA (LAMTOR1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Oct. 22, 2010

Availability

Academic Institutions and Nonprofits only
pCMV5 Flag-Smad1 (AAVA)

Plasmid

#14957

Depositor

Joan Massague

Publication

Kretzschmar et al Genes Dev. 1997 Apr 15. 11(8):984-95.

Insert

Smad1 (AAVA) (SMAD1 Human)

Tags

Flag

Expression

Mammalian

Mutation

Three Serine to Alanine mutations in the C-termin…

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNA3-Flag Rac2

Plasmid

#12192

Depositor

Gary Johnson

Publication

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Tags

Flag

Expression

Mammalian

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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pBA707

Plasmid

#186238

Purpose

KanR-BBR1 entry vector for HR donor

Depositor

Jennifer Doudna

Publication

Adler et al Nat Microbiol. 2022 Dec;7(12):1967-1979.

Type

Empty backbone

Expression

Bacterial

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3_SARS2_XBB

Plasmid

#194494

Purpose

expressing SARS-CoV-2 XBB spike protein for pseudovirus production

Depositor

David Nemazee

Publication

Zhou et al Immunity. 2023 Mar 14;56(3):669-686.e7.

Insert

Spike (XBB) (S SARS-CoV-2)

Expression

Mammalian

Mutation

V83A, Y144-,H146Q, Q183E, V213E, G339H, L368I, R3…

Promoter

CMV

Available since

Jan. 11, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCMV-hATG7wt

Plasmid

#87867

Purpose

Express a wild type human Atg7/Apg7 in mammalian cells

Depositor

Eiki Kominami, Isei Tanida

Publication

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

ATG7 (ATG7 Human)

Expression

Mammalian

Promoter

CMV

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-PAX3-FOXO1/FKHR

Plasmid

#115526

Purpose

Mammalian expression construct

Depositor

Martine Roussel

Publication

Lam et al Mol Cell Biol. 1999 Jan;19(1):594-601.

Insert

Pax3-FOXO1/FKHR (FOXO1, Pax3 Mouse, Human)

Expression

Mammalian

Available since

Sept. 27, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pAAV-EF1a-GCaMP6s-WPRE-pGHpA

Plasmid

#67526

Purpose

"Fluorescent reporter for calcium imaging"

Depositor

Botond Roska

Publication

Wertz et al Science. 2015 Jul 3;349(6243):70-4. doi:

Insert

GCaMP6s

Use

AAV

Tags

6xHis, T7 tag, and Xpress epitope

Expression

Mammalian

Promoter

EF1a

Available since

July 23, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pTDpelB-C_sfYFPTwinStrep

Plasmid

#45944

Purpose

expresses P.putida codon optimized PelB-sfYFP-Twin-Strep fusion protein for periplasmic translocation via PelB signal sequence

Depositor

Thorben Dammeyer

Publication

Dammeyer et al Microb Cell Fact. 2013 May 20;12(1):49.

Insert

synthetic sfYFP (codon usage adapted to P.putida KT2440)

Tags

PelB export sequence and Twin-Strep-tag

Expression

Bacterial

Promoter

lacIq/Ptrc

Available since

Sept. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pMXs-IP GFP-Omp25

Plasmid

#38249

Depositor

Noboru Mizushima

Publication

Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

Insert

Omp25 (Synj2bp Rat)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

plasmid contains AA109-145 of rat Omp25/Synj2bp

Available since

Sept. 2, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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BasiginCD4d3+4-Comp-His-Stop

Plasmid

#36147

Purpose

EXPRESs plasmid for human erythrocyte encoding BSG/EMMPRIN/CD147 and CD4d3+4-Comp-His-Stop pentamer

Depositor

Gavin Wright

Publication

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

Basigin (BSG Human)

Tags

His tag, pentamerization domain of rat cartilagin…

Expression

Mammalian

Promoter

CMV

Available since

May 14, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CaV2.1 (pCR3.1)

Plasmid

#26573

Depositor

Diane Lipscombe

Publication

Richards et al Eur J Neurosci. 2007 Nov . 26(10):2950-6

Insert

Cacna1a (Cacna1a Mouse)

Expression

Mammalian

Available since

Nov. 29, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pRG2

Plasmid

#104174

Purpose

sgRNA expression

Depositor

Jin-Soo Kim

Publication

Kim et al Genome Biol. 2017 Nov 15;18(1):218. doi:

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

42 Articles

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pYPQ133B

Plasmid

#69283

Purpose

Golden Gate entry vector; 3rd gRNA under AtU3 promoter

Depositor

Yiping Qi

Publication

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Type

Empty backbone

Use

CRISPR

Available since

Sept. 30, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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