Addgene

We narrowed to 37,652 results for: KAN

Showing: 2151 - 2200 of 37652 results

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pGWT35S-AePHOT-Citrine

Plasmid

#220105

Purpose

Transient expression of AePHOT-Citrine in plant cells

Depositor

Yutaka Kodama

Article

Yong et al Photochem Photobiol. 2023 Oct 26. doi: 1

Insert

AePHOT-Citrine

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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E2_pENTR_R4-R3_HSPter-RPS5A-NLS

Plasmid

#193371

Purpose

nTALEN or nTALECD vector assembly, Entry clone for plants to express ORFs from entry clone with NLS under control of the RPS5A promoter and HSP terminator.

Depositor

Shin-ichi Arimura

Article

Hosoda et al bioRxiv 2023.02.27.530312

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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INT_SS9sp

Plasmid

#212145

Purpose

Effector plasmid for for operon integration into the genome (Safe Site 9) using CRISPR-Cas12a. Plasmid can be removed by incubating cells at 37 C.

Depositor

Kang Zhou

Insert

VchTniQ, VchCas8, VchCas7, VchCas6, VchTnsA, VchTnsB, VchTnsC

Use

Tags

Expression

Bacterial

Mutation

Promoter

pJ23119

Available since

Jan. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(E224Q, GiVL-2)

Plasmid

#32035

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Use

Tags

His6-SUMO

Expression

Bacterial

Mutation

E224Q and N195-M215 replaced by P697-D714 of GiDi…

Promoter

T7

Available since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pMOD3-mCardinal-Sh_ble

Plasmid

#120334

Purpose

Bacterial mCardinal promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mCardinal

Use

Tags

Expression

Bacterial

Mutation

Promoter

(None)

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD3-mNeptune2-nat

Plasmid

#120335

Purpose

Bacterial mNeptune2 promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mNeptune2

Use

Tags

Expression

Bacterial

Mutation

Promoter

(None)

Available since

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD3-mNeptune2-Sh_ble

Plasmid

#120336

Purpose

Bacterial mNeptune2 promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mNeptune2

Use

Tags

Expression

Bacterial

Mutation

Promoter

(None)

Available since

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA3mt

Plasmid

#140464

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Insert

R-CEPIA3mt

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV R-CEPIA4mt

Plasmid

#140463

Purpose

Red fluorescent indicator for calcium imaging in the mitochondria (low affinity variant)

Depositor

Masamitsu Iino

Article

Kanemaru et al Sci Rep. 2020 Feb 18;10(1):2835. doi: 10

Insert

R-CEPIA4mt

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAXY0014

Plasmid

#205583

Purpose

HYG(1-89)-NpuDnaE(N)_GFPuv

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(N)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Tol2-ISceI-ZSP:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194518

Purpose

ZSP promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMK437 (mAID-mCherry-P2A-BSD)

Plasmid

#168232

Purpose

C-terminal tagging with mAID-mCherry

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-mCherry-P2A-BSD

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRS405-LEU2-TEF-KpolDcr1v2

Plasmid

#85193

Purpose

Integrating plasmid, K. polysporus Dcr1 under TEF promoter

Depositor

David Bartel

Article

Nakanishi et al Nature. 2012 Jun 20;486(7403):368-74. do

Insert

Dcr1

Use

Integrating, shuttle vector

Tags

Expression

Yeast

Mutation

Promoter

TEF

Available since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pMK434 (mAID-mClover-P2A-Hygro)

Plasmid

#168229

Purpose

C-terminal tagging with mAID-mClover

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-mClover-P2A-Hygro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-ISceI-E1b:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#193851

Purpose

E1b promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-ISceI-fos:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194516

Purpose

fos promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD3-proD/Lux-nat

Plasmid

#120324

Purpose

Bacterial luminescence

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

Lux operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

proD

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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ISA431: aTc-MP6

Plasmid

#189935

Purpose

Mutagenesis Plasmid: pTet, danQ926, dam, seqA, emrR, ugi and cda1, tetR, CloDF13, KanR

Depositor

Nathan Crook

Article

Al'Abri et al Nucleic Acids Res. 2022 Jun 10;50(10):e5

Insert

danQ926, dam, seqA, emrR, ugi and cda1

Use

Tags

Expression

Bacterial

Mutation

Promoter

pTet

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMK436 (mAID-mCherry-P2A-Hygro)

Plasmid

#168231

Purpose

C-terminal tagging with mAID-mCherry

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

mAID-mCherry-P2A-Hygro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD3-mClover-nat

Plasmid

#120328

Purpose

Bacterial mClover promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mClover

Use

Tags

Expression

Bacterial

Mutation

D103N (see comments below)

Promoter

(None)

Available since

April 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
Tol2-ISceI-P2:EGFP;cryaa:mCherry-ISceI-Tol2

Plasmid

#194515

Purpose

P2 promoter for transgenic enhancer assays in zebrafish

Depositor

Junsu Kang

Article

Begeman et al Dev Biol. 2022 Sep 24;492:47-58. doi: 10

Insert

Enhanced Green Fluorescent Protein

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-mCD8mCherry

Plasmid

#170206

Purpose

Gateway middle entry vector that encodes mCD8mCherry

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

mCD8

Use

Zebrafish

Tags

mCherry

Expression

Mutation

Promoter

none

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET28a-Rx3(A7)

Plasmid

#35525

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

His6-Rx3(A7)

Use

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available since

April 5, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD3-proD/mNeptune2-nat

Plasmid

#120337

Purpose

Bacterial mNeptune2 labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

mNeptune2

Use

Tags

Expression

Bacterial

Mutation

Promoter

proD

Available since

Aug. 31, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pProm1_BCD1-GFP

Plasmid

#80649

Purpose

Promoter1 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

NONE

Promoter

constitutive promoter

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pB7WG_ASX

Plasmid

#98891

Purpose

Plant expression vector for multigene cloning

Depositor

Kristina Gruden

Article

Lukan et al PLoS One. 2018 Jan 4;13(1):e0190526. doi

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Jan. 8, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
JBEI-12050

Plasmid

#84982

Purpose

Isopentenol production

Depositor

Taek Soon Lee

Article

Kang et al Metab Eng. 2016 Mar;34:25-35. doi: 10.10

Insert

MevTo-T21-MK-aphA

Use

Tags

Expression

Bacterial

Mutation

Promoter

LacUV5

Available since

July 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a-Vx3(A7)

Plasmid

#35526

Depositor

Robert Cohen

Article

Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

Insert

Vx3(A7)

Use

Tags

His6

Expression

Bacterial

Mutation

Changed G2 to P

Promoter

T7

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD3-Lux-nat

Plasmid

#120323

Purpose

Bacterial luminescence promoter trap

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

Lux operon

Use

Tags

Expression

Bacterial

Mutation

Promoter

(None)

Available since

July 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL2_136

Plasmid

#199083

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMK438 (Stag-3FLAG-P2A-Hygro)

Plasmid

#168233

Purpose

C-terminal tagging with Stag-3FLAG

Depositor

Masato Kanemaki

Article

Saito et al Curr Protoc. 2021 Aug;1(8):e219. doi: 10

Insert

Stag-3FLAG-P2A-Hygro

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 21, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
Pado Red pcDNA3.1

Plasmid

#79665

Purpose

Pado with mKate2

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

Pado mKate2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pProm11_BCD1-GFP

Plasmid

#80656

Purpose

Promoter11 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

NONE

Promoter

constitutive promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
Pado pcDNA3.1

Plasmid

#79663

Purpose

Proton Channel Fluorescent Voltage Indicator

Depositor

Bradley Baker

Article

Kang et al Sci Rep. 2016 Apr 4;6:23865. doi: 10.103

Insert

Pado Green

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF/myc/ER FLIPglu-3.2mDelta13V

Plasmid

#18019

Depositor

Wolf Frommer

Article

Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

Insert

FLIPglu F16A S112A Delta13

Use

Tags

ECFP and Venus

Expression

Mammalian

Mutation

F16A, S112A in binding protein

Promoter

Available since

Feb. 17, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
EF2451_pET21a(+)

Plasmid

#13840

Depositor

SeWon Suh

Article

Kang et al Acta Crystallograph Sect F Struct Biol C

Insert

PPAT

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
pProm9_BCD1-GFP

Plasmid

#80655

Purpose

Promoter9 with BCD1-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

NONE

Promoter

constitutive promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pMOD3-proD/tdTomato-hph

Plasmid

#120327

Purpose

Bacterial tdTomato labeling

Depositor

James Kirby

Article

Kang et al Infect Immun. 2017 Apr 21;85(5). pii: e0

Insert

tdTomato

Use

Tags

Expression

Bacterial

Mutation

Promoter

proD

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pProm1_BCD20-GFP

Plasmid

#80651

Purpose

Promoter1 with BCD20-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

NONE

Promoter

constitutive promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pProm1_BCD21-GFP

Plasmid

#80652

Purpose

Promoter1 with BCD21-gfp

Depositor

Nathan Hillson

Article

Linshiz et al ACS Synth Biol. 2014 Aug 15;3(8):515-24.

Insert

GFP

Use

P15a origin, constitutive promoter, kanr

Tags

Expression

Mutation

NONE

Promoter

constitutive promoter

Available since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pRGM10

Plasmid

#48790

Purpose

I-SceI DSB repair reporter cassette

Depositor

Michael Glickman

Article

Gupta et al Mol Microbiol. 2011 Jan;79(2):316-30. do

Inserts

lacZ(I-SceI)
lacZ-deltaN

Use

Dsb repair reporter

Tags

Expression

Mutation

Promoter

Available since

Feb. 3, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pMCV

Plasmid

#199743

Purpose

Minimal cloning vector

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAXY0012

Plasmid

#205581

Purpose

HYG(1-52)-NpuDnaE(N)_GFPuv

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(N)

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLEL_1 [βω]

Plasmid

#199057

Purpose

Even assembly end-linker

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

βω assembly linker

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLDV_42 [21o1G]

Plasmid

#199071

Purpose

Odd assembly vector with sfGFP dropout for Odd level assemblies

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL1_41

Plasmid

#199048

Purpose

Level 1 plasmid, barcoded ORI

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

2μ ORI plus NGS barcode 18

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLDV_8 [28o4G]

Plasmid

#199068

Purpose

Odd assembly vector with sfGFP dropout for Odd level assemblies

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLDV_21 [28o1*G]

Plasmid

#199069

Purpose

Odd assembly vector with sfGFP dropout for Odd level assemblies

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLDV_6 [28o2G]

Plasmid

#199066

Purpose

Odd assembly vector with sfGFP dropout for Odd level assemblies

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGLEL_2 [γω]

Plasmid

#199058

Purpose

Even assembly end-linker

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

γω assembly linker

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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