We narrowed to 2,540 results for: organization Addgene

1. pEGFP N1 mitoAURKA

   Plasmid

   #157754

   Purpose

   Expression of AURKA:GFP targeted to mitochondria

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   AURKA without the first 30 amino acids replaced b…

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV ShadowG-AURKA-mTurq2

   Plasmid

   #157768

   Purpose

   Expression of AuroraA kinase biosensor under CMV promoter

   Depositor

   Marc Tramier

   Article

   Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

   Insert

   AURKA (AURKA Human)
   

   Tags

   mTurquoise2 and shadowG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TVBB C-term-Dendra2-Puro

   Plasmid

   #169217

   Purpose

   Targeting vector backbone to support a knock-in of Linker-Dendra2-P2A-Puro at the C-terminus of a target locus

   Depositor

   Hugo Snippert

   Article

   Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

   Insert

   Double SapI flanked Dendra2-P2A-Puro
   

   Use

   Targeting vector backbone

   Available Since

   Jan. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLenti-VQRRA-P2A-GFP-PGK-Puro

   Plasmid

   #110864

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VQRRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VQRRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   D1135V, R1335Q, T1337R and NLS sequence at the N-…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-VRERRA-P2A-GFP-PGK-Puro

   Plasmid

   #110865

   Purpose

   Lentiviral vector for constitutive expression of Cas9-VRERRA-P2A-GFP in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   Cas9-VRERRA
   

   Use

   Lentiviral

   Tags

   FLAG

   Mutation

   D1135V, G1218R, R1335E, T1337R, and NLS sequence …

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLenti-tetON-NKX2-1-R162del-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204191

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (R162del) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Deletion of R162

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pLenti-tetON-NKX2-1-I207M -EF1a-TagRFP-2A-tet3G

   Plasmid

   #204213

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (I207M) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Point mutation on I207M

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLenti-tetON-NKX2-1-Q175∗-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204211

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (Q175∗) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Point mutation on Q175* (*termination)

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-tetON-NKX2-1-R178∗-EF1a-TagRFP-2A-tet3G

   Plasmid

   #204210

   Purpose

   Doxycycline inducible lentiviral vector for human NKX2-1 (R178∗) overexpression

   Depositor

   Emma Rawlins

   Article

   Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

   Insert

   NKX2-1 (NKX2-1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   Point mutation on R178* (*termination)

   Promoter

   TetON

   Available Since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLenti-tetON-NKX2-1-I207F -EF1a-TagRFP-2A-tet3G

    Plasmid

    #204212

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (I207F) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NK2 homeobox 1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Tags

    Point mutation on I207F

    Expression

    Mammalian

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMeCP2G pc1121

    Plasmid

    #167566

    Purpose

    The complete rat MeCp2 ORF is fused in frame of the NH2-terminus of the enhanced GFP (isolated from pEGFP-N1 vector; CLONTECH Laboratories, Inc.)

    Depositor

    Cristina Cardoso

    Article

    Brero et al J Cell Biol. 2005 Jun 6;169(5):733-43. d

    Inserts

    Methyl-CpG binding protein 2 (Mecp2 Rat)
    GFP
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET15b MOSPD2 [315-445]

    Plasmid

    #186474

    Purpose

    Expression of human MOSPD2 (MSP domain from residue 315 to 445), fused to a cleavable 6HIS tag, in bacteria

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO J. 2020 Dec 1;39(23):e104369. doi:

    Insert

    MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)
    

    Tags

    6HIS

    Expression

    Bacterial

    Mutation

    MSP domain [315-445]

    Available Since

    July 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCMV ShadowY-AURKA-mTurq2

    Plasmid

    #157770

    Purpose

    Expression of AuroraA kinase biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and shadowY

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA 3.1 mitoAURKA Lys162Met

    Plasmid

    #157752

    Purpose

    Expression of kinase-dead AURKA K62M in mitochondria

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP N1 AURKA 1-110

    Plasmid

    #157755

    Purpose

    Expression of AURKA Nter (aa1-110):GFP in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The first 110 amino acids of AURKA were cloned

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pEGFP N1 AURKA 1-30

    Plasmid

    #157756

    Purpose

    Expression of AURKA Nter (aa1-30):GFP in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The first 30 amino acids of AURKA were cloned

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA 3.1 AURKA deltaNter

    Plasmid

    #157749

    Purpose

    Expression of AURKA without Nter in mammalian cells

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Expression

    Mammalian

    Mutation

    the first 30AA of Aurora A are lacking

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLenti-VRER-P2A-GFP-PGK-Puro

    Plasmid

    #110862

    Purpose

    Lentiviral vector for constitutive expression of Cas9-VRER-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-VRER
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    D1135V, G1218R, R1335E, T1337R

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLenti-HF1-P2A-GFP-PGK-Puro

    Plasmid

    #110863

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1-P2A-GFP (not codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    N497A, R661A, Q695A, Q926A

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Lenti-FAB(PX-LD)

    Plasmid

    #214422

    Purpose

    Detection of peroxisome-lipid droplet contact site

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    PMP34-FKBP-V5-RspAN_IRES_CFAST-20nm-Halo-6xHp NC (SLC25A17 Human)
    

    Use

    Lentiviral

    Tags

    CFAST-Halo and FKBP-V5-RspAN

    Promoter

    CMV

    Available Since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. W45A mutant of H3K9me3 biosensor

    Plasmid

    #120808

    Purpose

    FRET biosensor. Disrupting HP1 binding capability in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1(W45A mutation)-EV linker-ECFP-mouse histone H3
    

    Expression

    Mammalian

    Mutation

    Tryptophan 45 is mutated to Alanine 45 on HP1 dom…

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. K9L mutant of H3K9me3 biosensor

    Plasmid

    #120807

    Purpose

    FRET biosensor. Eliminating the H3 lysine 9 methylation site in H3K9me3 biosensor to abolish the detection capability

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3(K9L mutation)
    

    Expression

    Mammalian

    Mutation

    lysine 9 is mutated to leucine 9 on histone H3

    Promoter

    CMV

    Available Since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSS02 NAPstar3B

    Plasmid

    #241225

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar3B in Arabidopsis thaliana

    Depositor

    Markus Schwarzländer

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    cyt-NAPstar3B
    

    Expression

    Plant

    Available Since

    Sept. 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pSS02 NAPstar2

    Plasmid

    #241272

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar2 in Arabidopsis thaliana

    Depositor

    Markus Schwarzländer

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    cyt-NAPstar2
    

    Expression

    Plant

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSS02 NAPstar7

    Plasmid

    #241224

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar7 in Arabidopsis thaliana

    Depositor

    Markus Schwarzländer

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    cyt-NAPstar7
    

    Expression

    Plant

    Available Since

    Sept. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSS02 NAPstarC

    Plasmid

    #241271

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstarC in Arabidopsis thaliana

    Depositor

    Markus Schwarzländer

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    cyt-NAPstarC
    

    Expression

    Plant

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pSS02 NAPstar6

    Plasmid

    #241223

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar6 in Arabidopsis thaliana

    Depositor

    Markus Schwarzländer

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    cyt-NAPstar6
    

    Expression

    Plant

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSS02 NAPstar3

    Plasmid

    #241273

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar3 in Arabidopsis thaliana

    Depositor

    Markus Schwarzländer

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    cyt-NAPstar3
    

    Expression

    Plant

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSS02 NAPstar4.3

    Plasmid

    #241222

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar4.3 in Arabidopsis thaliana

    Depositor

    Markus Schwarzländer

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    cyt-NAPstar4.3
    

    Expression

    Plant

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSS02 NAPstar1

    Plasmid

    #241270

    Purpose

    Expresses NADPH/NADP+ biosensor NAPstar1 in Arabidopsis thaliana

    Depositor

    Markus Schwarzländer

    Article

    Scherschel et al Nat Commun. 2024 Dec 19;15(1):10704. doi

    Insert

    cyt-NAPstar1
    

    Expression

    Plant

    Available Since

    Aug. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLenti-tetON-NKX2-1(delDBD)-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204214

    Purpose

    Doxycycline inducible lentiviral vector for human NKX2-1 (delDBD) overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    NKX2-1 (NKX2-1 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    Deletion of DNA binding domain (delDBD)

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. MSCV-human Erbb2-IRES-GFP

    Plasmid

    #91888

    Purpose

    Retroviral expression of human Erbb2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Erbb2 (ERBB2 Human)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Promoter

    MMLV gag

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. MSCV-mouse Ezh2 Y637S-IRES-GFP

    Plasmid

    #91887

    Purpose

    Retroviral expression of mouse Ezh2 with Y637S

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Ezh2 (Ezh2 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Y637S

    Promoter

    MMLV gag

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. MSCV-mouse Ezh2 delta SETdomain-IRES-GFP

    Plasmid

    #91885

    Purpose

    Retroviral expression of mouse Ezh2 with a SET domain deletion

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    Ezh2 (Ezh2 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    SET domain deleted

    Promoter

    MMLV gag

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. AOI-WT-Cas9-sg-mouse Ezh2-E10-GFP

    Plasmid

    #91879

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

36. AOI-WT-Cas9-sg-mouse Ezh2-E18-GFP

    Plasmid

    #91880

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Ezh2

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Ezh2 (Ezh2 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

37. AOI-WT-Cas9-sg-mouse Gfi1-exon5 (F3)-GFP

    Plasmid

    #91884

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Gfi1 (Gfi1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 19, 2017

    Availability

    Academic Institutions and Nonprofits only

38. AOI-WT-Cas9-sg-mouse Gfi1-exon4(F2)-GFP

    Plasmid

    #91877

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Gfi1 (Gfi1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

39. AOI-WT-Cas9-sg-mouse Gfi1-exon4(F1)-GFP

    Plasmid

    #91883

    Purpose

    Expresses 3xFLAG-Cas9 and a gRNA targeting mouse Gfi1

    Depositor

    Martine Roussel

    Article

    Vo et al Cell Rep. 2017 Mar 21;18(12):2907-2917.

    Insert

    gRNA targeting Gfi1 (Gfi1 Mouse)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    June 26, 2017

    Availability

    Academic Institutions and Nonprofits only

40. pcDNA3-WT-WLS-HA-IRES-GFP

    Plasmid

    #178061

    Purpose

    Expression of WLS-HA protein (WT)

    Depositor

    Joseph Gleeson

    Article

    Chai et al N Engl J Med. 2021 Sep 30;385(14):1292-1

    Insert

    WLS (WLS Human)
    

    Tags

    HA and IRES-GFP

    Expression

    Mammalian

    Available Since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. pCDH-EF1a-hSNCA

    Plasmid

    #170445

    Purpose

    Lentiviral vector expressing human SNCA, under control of EF1alpha promoter.

    Depositor

    Sang-Hun Lee

    Article

    Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

    Insert

    human SNCA (SNCA Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

    Plasmid

    #196196

    Purpose

    homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

    Depositor

    Jürgen Knoblich

    Article

    Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

    Insert

    G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
    

    Use

    Homology repair donor

    Tags

    V5-APEX2-mScarlet-I

    Available Since

    April 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pK029.CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #69138

    Purpose

    For the Supernova, a series of vector systems for single-cell labeling and gene function sudies in vivo

    Depositor

    Takuji Iwasato

    Article

    Mizuno et al Neuron. 2014 Apr 16;82(2):365-79. doi: 1

    Insert

    CAG-loxP-stop-loxP-RFP-ires-tTA-WPRE
    

    Tags

    none

    Expression

    Mammalian

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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44. pLenti-tetON-TFAP2C-EF1a-TagRFP-2A-tet3G

    Plasmid

    #204190

    Purpose

    Doxycycline inducible lentiviral vector for human TFAP2C overexpression

    Depositor

    Emma Rawlins

    Article

    Lim et al Cell Stem Cell. 2023 Jan 5;30(1):20-37.e

    Insert

    TFAP2C (TFAP2C Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    TetON

    Available Since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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45. pCMV superYFP-AURKA K162M-mTurq2

    Plasmid

    #157773

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and superYFP

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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46. pET22b-GST-6P2-LTLT-KIF1C(642–922)-GFP-6His

    Plasmid

    #222288

    Purpose

    Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal GFP and His tag in E.coli for purification.

    Depositor

    Anne Straube

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Insert

    KIF1C (KIF1C Human)
    

    Tags

    6xHis, GFP, and GST

    Expression

    Bacterial

    Mutation

    stalk region only amino acids 642 to 922

    Promoter

    T7

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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47. pET22b-GST-6P2-LTLT-KIF1C(642–922)-6His

    Plasmid

    #222289

    Purpose

    Expression of KIF1C stalk with N-terminal GST (cleavable) and C-terminal 6xHis tag in E.coli for purification.

    Depositor

    Anne Straube

    Article

    Abid Ali et al Nat Struct Mol Biol. 2025 Apr;32(4):756-

    Insert

    KIF1C (KIF1C Human)
    

    Tags

    6His and GST

    Expression

    Bacterial

    Mutation

    stalk region only amino acids 642 to 922

    Promoter

    T7

    Available Since

    June 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pCMV ShadowG-AURKA K162M-mTurq2

    Plasmid

    #157769

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and shadowG

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. pCMV ShadowY-AURKA K162M-mTurq2

    Plasmid

    #157771

    Purpose

    Expression of AuroraA kinase-dead biosensor under CMV promoter

    Depositor

    Marc Tramier

    Article

    Bertolin et al eLife. 2018 Aug 2;7. pii: 38111. doi: 10

    Insert

    AURKA (AURKA Human)
    

    Tags

    mTurquoise2 and shadowY

    Expression

    Mammalian

    Mutation

    AURKA K162M is a kinase-dead version of AURKA

    Promoter

    CMV

    Available Since

    Feb. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. CAG-Cas9-T2A-EGFP-ires-puro

    Plasmid

    #78311

    Purpose

    Expresses WT SpCas9, EGFP and Puromycin resistance from a CAG promoter.

    Depositor

    Timo Otonkoski

    Article

    Saarimaki-Vire et al Cell Rep. 2017 Apr 11;19(2):281-294. doi

    Insert

    WT SpCas9-T2A-EGFP-ires-puromycin resistance
    

    Use

    CRISPR

    Tags

    T2A-EGFP-ires-puro

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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