-
Plasmid#127344PurposeUntagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vectorDepositorInsertCyclin Dependent Kinase 13 (Cdk13 Mouse)
UsePiggybac transposase vectorTagsNoneExpressionMammalianMutationBase pairs 1-1554 of transgene are codon optimize…PromoterTetO PromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-Neo-TetO-Cdk13_Nterm_FlagHa
Plasmid#127345PurposeN-terminal Flag- HA- tandem epitope tagged mouse Cdk13 transgene (NP_001074527.1 isoform) cloned into a dox-inducible piggybac destination vectorDepositorInsertCyclin Dependent Kinase 13 (Cdk13 Mouse)
UsePiggybac transposase vectorTagsFlag- HA- Tandem EpitopesExpressionMammalianMutationBase pairs 1-1554 of transgene are codon optimize…PromoterTetO PromoterAvailable sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL2620 MTK3-TetR
Plasmid#226670PurposeMTK3 part containing the coding sequence of TetRDepositorInsertTetR
UseSynthetic BiologyTagsExpressionMutationPromoternoneAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-human cGASdeltaN-HA
Plasmid#186897PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertHuman cGAS truncation mutant (160-522 a.a.) with C-terminal HA tag (Adamts1 Synthetic, Human)
UseRetroviralTagsHAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTetOne-orangutan cGASdeltaN-HA
Plasmid#186898PurposeDoxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transductionDepositorInsertOrangutan cGAS truncation mutant (158-521 a.a.) with C-terminal HA tag (CGAS Pongo abelii, Synthetic)
UseRetroviralTagsHAExpressionMutationN-terminal truncationPromoterTRE3GAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-scFVtet1_bGHpA
Plasmid#177357PurposeAAV expression of scFV-fused catalytic domain of TET1 from doxycycine-inducible promoter for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 1 (TET1 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationN terminal domain of human Tet1PromoterTetracycline-dependent promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-TRE-scFVtet2_bGHpA
Plasmid#177358PurposeAAV expression of scFV-fused catalytic domain of TET2 from doxycycine-inducible promoter for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 2 (TET2 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationCatalytic domains of human TET2 (1129–1936 and 14…PromoterTetracycline-dependent promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-CMV-scFVtet2_bGHpA
Plasmid#177352PurposeAAV expression of scFV-fused catalytic domain of TET2 for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 2 (TET2 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationCatalytic domains of human TET2 (1129–1936 and 14…PromoterCMV promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSyn-scFVtet2_bGHpA
Plasmid#177353PurposeAAV expression of scFV-fused catalytic domain of TET2 from Synapisin promoter for targeted DNA methylation editingDepositorInsertTet Methylcytosine Dioxygenase 2 (TET2 Human)
UseAAVTagsmyc and scFVExpressionMammalianMutationCatalytic domains of human TET2 (1129–1936 and 14…Promoterhuman Synapsine promoterAvailable sinceSept. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-Tet-O-EYA2-Puro
Plasmid#221669PurposeExpresses EYA2 in mammalian cellsDepositorInsertEYA2 (EYA2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
p-Tet-O-Foxp1A-Puro
Plasmid#221665PurposeExpresses Foxp1A in mammalian cellsDepositorInsertFoxp1A (Foxp1 Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mTet1s pc3901
Plasmid#201700PurposeExpresses of GFP-tagged mTet1s in mammalian cellsDepositorInsertTet1s (Tet1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmcherry-mTet1s pc3905
Plasmid#201703PurposeExpresses of mcherry-tagged mTet1s in mammalian cellsDepositorInsertTet1s (Tet1 Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCAGAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mTet1s_K852R pc3914
Plasmid#201704PurposeExpresses of GFP-tagged mTet1s_K852R in mammalian cellsDepositorInsertTet1s-K852R (Tet1 Mouse)
UseTagsGFPExpressionMammalianMutationLysine 852 mutated to argininePromoterCAGAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGFP-mTet1s_K852E pc3915
Plasmid#201705PurposeExpresses of GFP-tagged mTet1s_K852E in mammalian cellsDepositorInsertTet1s-K852E (Tet1 Mouse)
UseTagsGFPExpressionMammalianMutationLysine 852 mutated to glutamatePromoterCAGAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmch-mTet1-ZF-CD pc3956
Plasmid#201709PurposeExpresses of mcherry-tagged mTet1-ZF-CD in mammalian cellsDepositorInsertTet1-ZF_CD (Tet1 Mouse)
UseTagsmCherryExpressionMammalianMutationZinc finger domain of Tet1 fused to its catalytic…PromoterCAGAvailable sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Alternate Quartet
Plasmid#214728PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses pang17, RmYN02, Rf1, and WIV1 each separated by GS-linkers with an N-terminal SpyTag fusionDepositorInsertSpyTag-Alternate Quartet
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Quartet [SARS1]
Plasmid#214729PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV each separated by GS-linkers with an N-terminal SpyTag fusionDepositorInsertSpyTag-Quartet [SARS1] (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
SpyTag-Kraken Quartet
Plasmid#214730PurposeIn mammalian cells expresses a Quartet of receptor-binding domains (RBDs) from the sarbecoviruses SHC014, Rs4081, RaTG13, and SARS-CoV-2 XBB.1.5 separated by GS-linkers with an N-terminal SpyTagDepositorInsertSpyTag-Kraken Quartet (S Synthetic)
UseTagsSpyTagExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
EF1a-hP53DD (TET)
Plasmid#214083PurposeMammalian expression tetramerization domain (TET) of human TP53DepositorInserthP53DD (TP53 Human)
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only