We narrowed to 3,251 results for: actin

1. pET28b_RanWT

   Plasmid

   #186270

   Purpose

   Expression vector of His-RanQ69L in E.coli

   Depositor

   Masahito Ikawa

   Article

   Mori et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

   Insert

   GTP-binding nuclear protein Ran (Ran Mouse)
   

   Tags

   His tagged

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. plko-shmfn2-mito-GFP-ER tethering

   Plasmid

   #160509

   Purpose

   Rescue mfn2 knockdown by tethering mitochondria and ER with GFP

   Depositor

   Qing Deng

   Article

   Zhou et al J Cell Sci. 2020 Sep 4;133(17). pii: jcs

   Insert

   mito-GFP-ER tethering
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJJR82

   Plasmid

   #75027

   Purpose

   EGFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Article

   Ramalho et al Development. 2020 Jul 22;147(14):dev1880

   Insert

   EGFP^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized EGFP

   Expression

   Worm

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJJR83

   Plasmid

   #75028

   Purpose

   mCherry^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Mike Boxem

   Article

   Ramalho et al Development. 2020 Jul 22;147(14):dev1880

   Insert

   mCherry^SEC^3xFlag
   

   Use

   CRISPR

   Tags

   3xFLAG and C. elegans codon optimized mCherry

   Expression

   Worm

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBA-mKeima-PIM

   Plasmid

   #174650

   Purpose

   Low level expression of mKeima-PIM. Can be used for ratiometric detection of inducible aggregate acidification after addition of AP20187. mKeima fluorophore allows for simultaneous GFP-tagged imaging.

   Depositor

   Lukas Kapitein

   Article

   Janssen et al J Cell Sci. 2021 Oct 1;134(19). pii: 271

   Insert

   xXFKBP homo-mKeima-2x FKBP homo-2x FKBP
   

   Expression

   Mammalian

   Mutation

   Val24Glu, Tyr80Cys, Ala95Thr mutations in second …

   Promoter

   Chicken beta-actin

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. Myo1E-pEGFP

   Plasmid

   #124658

   Purpose

   Mouse myosin-1e fused with EGFP

   Depositor

   Cheng-han Yu

   Article

   Zhang et al Mol Biol Cell. 2019 Mar 1;30(5):622-635.

   Insert

   Myo1e (Myo1e Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Available Since

   May 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEGFP TEM4 FL

   Plasmid

   #58893

   Purpose

   Expresses GFP tagged full length TEM4 protein. Please see notes below for information regarding the L722F mutation in TEM4.

   Depositor

   Channing Der

   Article

   Mitin et al PLoS One. 2012;7(7):e41876. doi: 10.1371

   Insert

   TEM4 (ARHGEF17 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L722F

   Promoter

   CMV

   Available Since

   Sept. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBa.Halo-Kif13B tail 442-1843

   Plasmid

   #162721

   Purpose

   Mammalian expression of WT mouse Kif13b with deletion of the 441 N-terminal residues

   Depositor

   Marvin Bentley

   Article

   Frank et al Traffic. 2020 Sep 21. doi: 10.1111/tra.1

   Insert

   Kif 13B (Kif13b Mouse)
   

   Expression

   Mammalian

   Mutation

   WT with deletion of the 441 N-terminal residues.

   Promoter

   Chicken Beta actin

   Available Since

   Dec. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti CMVie-IRES-BlastR alt MCS

   Plasmid

   #120862

   Purpose

   3rd generation lentiviral expression under a CMVie promoter with Blasticidin selection

   Depositor

   Ghassan Mouneimne

   Article

   Puleo et al J Cell Biol. 2019 Oct 8. pii: jcb.201902

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV immediate early

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB_Cdc42-TomKat-sensor-CAAX

    Plasmid

    #172473

    Purpose

    Piggybac vector for mammalian expression of TomKat (tdTomato-tdKatushka) FRET sensor for Cdc42 activity anchored to the membrane with the polybasic and CAAX motif from Kras.

    Depositor

    Sean R Collins

    Article

    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

    Insert

    tdKatushka2-PBD(Pak1)-EVlinker-Cdc42-tdTomato-CAAX
    

    Use

    Piggybac transposon

    Tags

    polybasic and CAAX motif from Kras, tdKatushka2, …

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCB6-Cor1-HA

    Plasmid

    #102815

    Purpose

    Expression of Coronin1 in eukaryotic cells

    Depositor

    Jean Pieters

    Article

    Gatfield et al Mol Biol Cell. 2005 Jun;16(6):2786-98. E

    Insert

    TACO/Coronin 1 (Coro1a Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV6M-Pak1 H83L H86L

    Plasmid

    #12211

    Depositor

    Jonathan Chernoff

    Article

    Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

    Insert

    Pak1 (PAK1 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    PBD (p21-binding domain) mutant: H83L, H86L (see …

    Available Since

    Aug. 11, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV_PP1-mCitrine

    Plasmid

    #172472

    Purpose

    Mammalian expression of a human codon-optimized version of the optogenetic GPCR parapinopsina (PP1) from zebrafish fused to mCitrine. Also contains an N-terminal prolactin signal sequence.

    Depositor

    Sean R Collins

    Article

    Bell et al Nat Commun. 2021 Nov 16;12(1):6148. doi:

    Insert

    prolactinSS-PP1-mCitrine (parapinopsina Zebrafish)
    

    Use

    Lentiviral

    Tags

    mCitrine and prolactin signal sequence (cleaved p…

    Expression

    Mammalian

    Promoter

    EF1alpha

    Available Since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. mCherry-PH-SWAP70

    Plasmid

    #84582

    Purpose

    Expresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

    Insert

    SWAP70 residues 209-306 (SWAP70 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Pleckstrin-homology domain, residues 209-306

    Promoter

    CMV

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pCAG-GFP-PQRv3-RFP

    Plasmid

    #73951

    Purpose

    Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

    Depositor

    Brian Chen

    Article

    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

    Inserts

    sfGFP (superfolder GFP)
    RFP
    

    Tags

    A residual PQR peptide wii be left at the C-termi…

    Expression

    Mammalian

    Promoter

    Chicken beta actin and Chicken beta actin (shared…

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only

16. pCS2+MT-hToca-1-MGD

    Plasmid

    #33033

    Depositor

    Marc Kirschner

    Article

    Ho et al Cell. 2004 Jul 23;118(2):203-16.

    Insert

    Toca-1 (FNBP1L Human)
    

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    MGD = amino acid substitutions M383I, G384S and D…

    Promoter

    simian CMV IE94

    Available Since

    Nov. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pmiR-report-hEZH2

    Plasmid

    #112543

    Purpose

    3'UTR reporter assay

    Depositor

    Shusheng Wang

    Article

    Zhou et al Mol Ther. 2014 Feb;22(2):378-389. doi: 1

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Luciferase

    Available Since

    July 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGEX-6P-2_dCrk

    Plasmid

    #131146

    Purpose

    Expresses GST::dCrk (based on crk isoform RA).

    Depositor

    Mark Peifer

    Article

    Spracklen et al Mol Biol Cell. 2019 Jul 18:mbcE19050302.

    Insert

    Crk (Crk Fly)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV5-Flag-EPLIN delta N-term

    Plasmid

    #40932

    Depositor

    Elizabeth Luna, Scott Maul

    Article

    Song et al Mol Biol Cell. 2002 Apr;13(4):1408-16.

    Insert

    EPLIN (LIMA1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-220

    Available Since

    Dec. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pFX-Lifeact-mCherry

    Plasmid

    #175822

    Purpose

    Express mCherry with actin-targeting sequence in mammalian cells

    Depositor

    Yusuke Ohba

    Article

    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

    Insert

    mCherry with actin-targeting sequence
    

    Tags

    Lifeact (N-terminal 17 amino acids from S. Cerevi…

    Expression

    Mammalian

    Promoter

    CMB

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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