We narrowed to 39,265 results for: lat

1. pBS-Cx43-FS

   Plasmid

   #65440

   Purpose

   Cloning, T7 RNA expression

   Depositor

   Trond Aasen

   Article

   Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

   Insert

   Cx43 (GJA1 Human)
   

   Use

   T7 in vitro rna production

   Tags

   Expression

   Bacterial

   Mutation

   Frameshift (FS) mutation 35delA

   Promoter

   T7

   Available since

   June 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-HA-mIFITM3-D92N

   Plasmid

   #58412

   Purpose

   Expresses murine IFITM3-D92N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

   Depositor

   Jacob Yount, Howard Hang

   Article

   Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

   Insert

   IFITM3 (Ifitm3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Apartate 92 to Asparagine

   Promoter

   CMV IE

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pCMV-HA-mIFITM3-N2A

   Plasmid

   #58410

   Purpose

   Expresses murine IFITM3-N2A with an N-terminal HA tag in mammalian cells (mutates possible glycosylation site)

   Depositor

   Jacob Yount, Howard Hang

   Article

   Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

   Insert

   IFITM3 (Ifitm3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Asparagine 2 to Alanine

   Promoter

   CMV IE

   Available since

   Sept. 8, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pCMV-HA-mIFITM3-S38N

   Plasmid

   #58411

   Purpose

   Expresses murine IFITM3-S38N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

   Depositor

   Jacob Yount, Howard Hang

   Article

   Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

   Insert

   IFITM3 (Ifitm3 Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Serine 38 to Asparagine

   Promoter

   CMV IE

   Available since

   Sept. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

5. pJK520

   Plasmid

   #155386

   Purpose

   PphlF-TA1 crRNAs, 2x cascade. dCas12a (D917A) + PA4-mVenus

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas12a (F. novicida)
   PA4-mVenus
   PphlF-TA1 in dCas12a oscillator crRNAs
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D917A (nuclease-deactivating)

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pmax pona 12xTRICK 24xMS2SL

   Plasmid

   #64542

   Purpose

   TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

   Depositor

   Jeffrey Chao

   Article

   Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

   Insert

   12x PP7 stem-loops
   

   Use

   Tags

   24xMS2 stem loops, HA, NLS, and dNP799

   Expression

   Mammalian

   Mutation

   Promoter

   ponA

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA3.1(+)- e18

   Plasmid

   #131670

   Purpose

   Expresses an engineered variant of RAD18 ("e18") that stimulates CRISPR mediated HDR.

   Depositor

   Alberto Ciccia

   Article

   Nambiar et al Nat Commun. 2019 Jul 30;10(1):3395. doi:

   Insert

   e18 (engineered RAD18 with SAP domain deleted) (RAD18 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET30-HPF1-His-Sumo-Flag

   Plasmid

   #111577

   Purpose

   Expresses full length HPF1 in E. coli

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   HPF1 (HPF1 Human)
   

   Use

   Tags

   6xHis-Sumo-FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1-MS2CP-VPg(FCV)

   Plasmid

   #167314

   Purpose

   Template pDNA to prepare CaVT mRNA

   Depositor

   Hirohide Saito

   Article

   Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

   Insert

   CaVT
   

   Use

   Synthetic Biology

   Tags

   DYK-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSB-TRE-CRISPRoff-EF1A-TetOn

    Plasmid

    #203355

    Purpose

    Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

    Depositor

    Jonathan Brunger

    Article

    Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

    Insert

    CRISPRoff-TetOn
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSicoR-mCh-empty

    Plasmid

    #21907

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

    Depositor

    Miguel Ramalho-Santos

    Article

    Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pX330a dCas9-LSD1

    Plasmid

    #92362

    Purpose

    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to LSD1. For targeted enhancer demethylation in chicken embryos.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    dCas9-LSD1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pShuttle-mRFP-gsk3 s9a

    Plasmid

    #24371

    Depositor

    Torsten Wittmann

    Article

    Kumar et al J Cell Biol. 2009 Mar 23. 184(6):895-908

    Insert

    GSK3beta(S9A) (GSK3B Human)
    

    Use

    Adenoviral

    Tags

    mRFP

    Expression

    Mammalian

    Mutation

    S9A. Mutant version of GSK3 that lacks the inhibi…

    Promoter

    Available since

    May 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV asyn WT

    Plasmid

    #36055

    Depositor

    Hilal Lashuel

    Article

    Oueslati et al J Neurosci. 2012 Feb 1;32(5):1536-44.

    Insert

    alpha-synuclein WT (SNCA Human)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1 AK3 Hyg

    Plasmid

    #37539

    Depositor

    David Sidransky

    Article

    Chang et al PLoS One. 2011;6(6):e20806. Epub 2011 Ju

    Insert

    AK3 (AK3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 14, 2012

    Availability

    Academic Institutions and Nonprofits only

16. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR

    Plasmid

    #67931

    Purpose

    Translational reporter - ARHGAP5 UTRs

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    sfGFP-NLS-P2A-NLS-mCherry (ARHGAP5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV_hSyn_SpikeyGi2_kv2.1

    Plasmid

    #195313

    Purpose

    Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, in AAV vector under the neuronal-specific promoter hSyn

    Depositor

    Vincent Pieribone

    Article

    Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

    Insert

    SpikeyGi2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV_Flex_syn_SpikeyGi2_kv2.1

    Plasmid

    #195314

    Purpose

    Genetically-encoded voltage indicator SpikeyGi2, soma and AIS targeted, double-flowed in AAV vector under the promoter hSyn

    Depositor

    Vincent Pieribone

    Article

    Platisa et al Nat Methods. 2023 Mar 27. doi: 10.1038/s

    Insert

    SpikeyGi2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human synapsin promoter

    Available since

    March 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)

    Plasmid

    #191647

    Purpose

    E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion

    Depositor

    Emily Balskus

    Article

    Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

    Insert

    CymR
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBCMV-HaloTag-VPg(FCV)

    Plasmid

    #167311

    Purpose

    Template pDNA to prepare a split CaVT component mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    HaloTag-VPg(FCV)
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge