We narrowed to 21,601 results for: ESC;

1. pAAV- CAG-h2BmRuby2FLAG

   Plasmid

   #116872

   Purpose

   Nuclear localized mRuby2FLAG

   Depositor

   Loren Looger

   Article

   Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

   Insert

   H2BmRuby2FLAG
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGBW-m4046910

   Plasmid

   #145672

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 nsp4

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 nsp4 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;StrepII

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 29, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV2_VP1_RaPID_r1c3

   Plasmid

   #65723

   Purpose

   Produce random mCherry insertion in AAV2 VP3 region of VP1; ITRs in VP1 only

   Depositor

   Junghae Suh

   Article

   Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

   Insert

   mCherry
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

4. Clover-Rab5a-7

   Plasmid

   #56530

   Purpose

   Localization: Ras GTPase, Excitation: 505, Emission: 515

   Depositor

   Michael Davidson

   Article

   Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

   Insert

   Rab5a
   

   Use

   Tags

   Clover

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 18, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pmScarlet-i_NES_C1

   Plasmid

   #85062

   Purpose

   In vivo visualization of the cytoplasm, but not the nucleus (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   NES
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNC1011

   Plasmid

   #42898

   Depositor

   Beverly Errede

   Article

   Houser et al Yeast. 2012 Dec;29(12):519-30. doi: 10.1

   Insert

   3 tandem copies of GFP*
   

   Use

   Yeast integrating

   Tags

   Expression

   Mutation

   changed Phenylalanine 64 to Leucine, Serine 65 t…

   Promoter

   None

   Available since

   March 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX330-sgRNA_Dgcr8_1

   Plasmid

   #73525

   Purpose

   Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8

   Depositor

   Constance Ciaudo

   Article

   Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

   Insert

   DGCR8
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Jan. 30, 2017

   Availability

   Academic Institutions and Nonprofits only

8. pAJT430

   Plasmid

   #202291

   Purpose

   RPU integration plasmid (LPattB7)

   Depositor

   Christopher Voigt

   Article

   Triassi et al Cell Syst. 2023 Jun 21;14(6):512-524.e12

   Insert

   J23101-RiboJ00-B0034-YFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRU292

   Plasmid

   #167687

   Purpose

   Final destination vector for single Gateway LR reaction

   Depositor

   Niko Geldner

   Article

   Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

   Insert

   PcUBQ4-2::Cas9i
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEB2-TagRFP

    Plasmid

    #103997

    Purpose

    Plasmid encoding TagRFP

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    TagRFP
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to eqFP578 (R32G, K42R, K67R, …

    Promoter

    proC

    Available since

    Dec. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV.Syn-FLEX.iGABASnFR.F102G.mRuby3

    Plasmid

    #112170

    Purpose

    GABA Sensor

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

    Insert

    iGABASnFR.F102G
    

    Use

    AAV

    Tags

    mRuby3

    Expression

    Mutation

    F102G

    Promoter

    Synapsin

    Available since

    July 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. mEos2-Golgi-7

    Plasmid

    #57382

    Purpose

    Localization: Golgi Complex, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Golgi (B4GALT1 Human)
    

    Use

    Tags

    mEos2

    Expression

    Mammalian

    Mutation

    aa 1-82 of NM_001497.3

    Promoter

    CMV

    Available since

    Feb. 5, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pZH520

    Plasmid

    #102673

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZH521

    Plasmid

    #102674

    Purpose

    Tetracycline inducible expression of GFPmut2 with constitutive TetR; weak ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

    Depositor

    Zach Hensel

    Article

    Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

    Insert

    GFPmut2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMito-dCherry-FRB

    Plasmid

    #186573

    Purpose

    Expression of "dark MitoTrap" - an FRB-domain targeted to the mitochondria that is the same size as pMito-mCherry-FRB but is non-fluorescent - in human cells

    Depositor

    Stephen Royle

    Article

    Kuey et al Elife. 2022 Jul 19;11. pii: 78929. doi:

    Insert

    Dark MitoTrap
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutation of "K70N" to render mCherry no…

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRU61

    Plasmid

    #167692

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    pEC1.2::asCpf1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. Lentiviral-KOR-FRB

    Plasmid

    #191496

    Purpose

    Rapamycin-dependent opioid sensor for the kappa opioid receptor (component 1)

    Depositor

    Wenjing Wang

    Article

    Kroning et al ACS Chem Biol. 2022 Aug 19;17(8):2212-22

    Insert

    KOR-FRB
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pNVLTv2-pvdIJD-Ptrc-base

    Plasmid

    #169243

    Purpose

    Suicide vector for integrating expression cassette in P. putida (IPTG induction)

    Depositor

    Brian Pfleger

    Article

    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:

    Insert

    lacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq

    Available since

    June 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pFLAG-ING2

    Plasmid

    #13295

    Depositor

    Curtis Harris

    Article

    Pedeux et al Mol Cell Biol. 2005 Aug . 25(15):6639-48

    Insert

    ING2 (INGX Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

20. PEP115E

    Plasmid

    #97401

    Purpose

    binary construct of Golgi-targeted mCherry with sfGFP11 tag for plant expression

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

    Insert

    mCherry-sfGFP11
    

    Use

    Tags

    Golgi target

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Sept. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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