We narrowed to 21,477 results for: SPR

Showing: 8821 - 8840 of 21477 results

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pDGE497

Plasmid

#153249

Purpose

M6E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pFH41

Plasmid

#125880

Purpose

Level0 Parsley PcUBI4-2 Pol II promoter

Depositor

Vladimir Nekrasov

Article

Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

Insert

Level0 Parsley PcUbi4-2 Pol II promoter

Use

CRISPR and Synthetic Biology

Expression

Plant

Available Since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pJL009

Plasmid

#122862

Purpose

Binary expression vector containing 35S:MS2-SRDX:tRBCS followed by GreenGate A-G sequences for insertion of additional modules

Depositor

Markus Schmid

Article

Lee et al PLoS One. 2019 Sep 26;14(9):e0222778. do

Insert

35S:MS2-SRDX:tRBCS

Use

Golden gate compatible plant transformation vector

Available Since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-28

Plasmid

#120007

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with 3xHA

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

3xHA

Use

Pcr template

Tags

3xHA

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pMaCTag-17

Plasmid

#119996

Purpose

Template for C-terminal PCR tagging of mammalian genes (without selection) with mEos4b

Depositor

Michael Knop

Article

Fueller et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

Insert

mEos4b

Use

Pcr template

Tags

mEos4b

Promoter

None

Available Since

Jan. 8, 2019

Availability

Academic Institutions and Nonprofits only

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BCJJ180

Plasmid

#117516

Purpose

Cas9_4 Golden Gate Level 0

Depositor

Jonathan D Jones

Article

Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

Insert

BsaI:AATG:Cas9_4:GCTT:BsaI

Use

CRISPR

Tags

3xFLAG and NLS

Expression

Plant

Available Since

Nov. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ157

Plasmid

#98413

Purpose

Pombe Expression Vector - nmt81 promoter with Sp.ura4 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ174

Plasmid

#98421

Purpose

Pombe Expression Vector - nmt1 promoter with Sp.lys9 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Expression

Yeast

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pYZ190

Plasmid

#98428

Purpose

Pombe General Purpose Vector - with Sp.ura4 marker

Depositor

Jef Boeke

Article

Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pGCG26

Plasmid

#82371

Purpose

YFP expressed from pJ23119 in the glpK locus with gentamicin resistance

Depositor

Brian Pfleger

Article

Gordon et al Metab Eng. 2016 Jul 29. pii: S1096-7176(

Insert

YFP

Promoter

PJ23119

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pJZC548

Plasmid

#62316

Purpose

sgRNA with 1x PP7 for yeast cells

Depositor

Wendell Lim, Stanley Qi

Article

Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01

Insert

sgRNA + 1x PP7

Expression

Yeast

Promoter

SNR52

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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pX552-hsyn DIO GCaMP8f(with gRNA scaffold)

Plasmid

#199580

Purpose

Expresses GCaMP8f in a Cre-dependent manner

Depositor

Bryan Copits

Article

Moffa et al eNeuro. 2024 Jul 5;11(7):ENEURO.0438-23.

Insert

His-DIO GCaMP8f

Use

AAV, CRISPR, and Cre/Lox

Tags

6xHis

Expression

Mammalian

Promoter

hsyn

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pUb-Cas9-mCherry_cU6:6-sgRNA

Plasmid

#190598

Purpose

The plasmid encodes S. pyogenes Cas9 and mCherry separated by a T2A peptide under an Aedes aegypti polyubiquitin promoter. It also expresses a sgRNA scaffold under a Culex quinquefasciatus U6 promoter

Depositor

Claudia Rueckert

Article

Torres et al Insects. 2022 Sep 19;13(9):856. doi: 10.

Inserts

Cas9
sgRNA

Use

CRISPR

Tags

mCherry - separated by T2A

Promoter

Aedes aegypti polyubiquitin and Culex quinquefasc…

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro

Plasmid

#192684

Purpose

Lentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Human IL1RN activating gRNAs #1,2,3 (IL1RN Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pDIV494

Plasmid

#177701

Purpose

Plasmid expressing optimized Cas9 and NAT marker and sgRNA targeting ADE2 locus in D. hansenii

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat
sgRNA Targeting ADE2 locus in D.hansenii: AGCTAAGCAGATTAATGCAT

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ), SNR52p (Candida s…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

Plasmid

#168107

Purpose

PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry

Depositor

Erich Jarvis

Article

Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10

Insert

Cas9-P2A-mCherry

Use

CRISPR and Cre/Lox

Tags

HA

Promoter

CAG

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pDGB3 alpha1 Tnos:nptII:Pnos - P35S:Cas9:Tnos - P35S:DsRed:Tnos (GB2234)

Plasmid

#160628

Purpose

Module for the constitutive expression of the nptII, Cas9 and DsRed genes.

Depositor

Diego Orzaez

Article

Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

Insert

tNos:nptII:PNos-P35s:Cas9:tNos-P35s:DsRed:tNos

Use

CRISPR

Mutation

BsaI and BsmBI sites removed

Promoter

Pnos, 35S, 35S

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH1

Plasmid

#164800

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA1

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA1
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.ERH2

Plasmid

#164801

Purpose

For CRISPRi knockdown of ERH by lentiviral delivery of ERH gRNA2

Depositor

David Bartel

Article

Fang et al Mol Cell. 2020 Apr 16;78(2):289-302.e6.

Inserts

ERH CRISPRi gRNA2
puro-T2A-BFP

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1Alpha and U6

Available Since

March 15, 2021

Availability

Academic Institutions and Nonprofits only

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BRD2 gRNA (BRDN0001148966)

Plasmid

#77985

Purpose

3rd generation lentiviral gRNA plasmid targeting human BRD2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

BRD2 (BRD2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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