We narrowed to 21,605 results for: ESC;

1. SBP-mCherry-Ecadherin_puromycin

   Plasmid

   #65293

   Purpose

   Ecadherin reporter only (no hook) (RUSH system)

   Depositor

   Franck Perez

   Article

   Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

   Insert

   Ecadherin
   

   Use

   Tags

   IL-2 Signal Sequence and Streptavidin Binding Pro…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. mOrange2-H2B-6

   Plasmid

   #57962

   Purpose

   Localization: Nucleus/Histones, Excitation: 549, Emission: 565

   Depositor

   Michael Davidson

   Article

   Kremers et al Nat Methods. 2009 May;6(5):355-8. doi: 1

   Insert

   H2B (H2BC11 Human)
   

   Use

   Tags

   mOrange2

   Expression

   Mammalian

   Mutation

   D26G and V119I in H2B

   Promoter

   CMV

   Available since

   Oct. 24, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pGBW-m4046959

   Plasmid

   #145722

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 ORF3a protein

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 ORF3a protein (ORF3a Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   April 27, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pGBW-m4046879

   Plasmid

   #149238

   Purpose

   Bacterial expression plasmid for SARS-CoV-2 nsp2

   Depositor

   Benjie Chen, Ginkgo Bioworks

   Insert

   SARS-CoV-2 nsp2 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available since

   May 12, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pMito.iGABASnFR.R205A

   Plasmid

   #112178

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.R205A
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R205A (no GABA binding)

   Promoter

   Available since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.CAG-FLEX.iGABASnFR.F102G

   Plasmid

   #112167

   Purpose

   GABA Sensor

   Depositor

   Loren Looger

   Article

   Marvin et al Nat Methods. 2019 Aug;16(8):763-770. doi

   Insert

   iGABASnFR.F102G
   

   Use

   AAV

   Tags

   Expression

   Mutation

   F102G

   Promoter

   CAG

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST-hPDIb′a′

   Plasmid

   #60556

   Purpose

   enhance solubility and correct disulfide bond-formation of fused protein expressed in E.coli

   Depositor

   Han Choe

   Article

   Song et al Appl Biochem Biotechnol. 2013 May;170(1)

   Type

   Empty backbone

   Use

   Tags

   His6-hPDIb'a' tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Nov. 4, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. mStrawberry-Vimentin-7

   Plasmid

   #55945

   Purpose

   Localization: Intermediate Filaments, Excitation: 574, Emission: 596

   Depositor

   Michael Davidson

   Article

   Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

   Insert

   Vimentin (VIM Human)
   

   Use

   Tags

   mStrawberry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFOX6-Tier1-PhCMV-TdTomato

   Plasmid

   #169515

   Purpose

   Tier-1 vector encoding PhCMV-driven bright-red fluorescent protein TdTomato (PhCMV-TdTomato-pA).

   Depositor

   Martin Fussenegger

   Article

   Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

   Insert

   PCMV-driven dimeric red fluorescent protein TdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PhCMV

   Available since

   June 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac1 PIK3R1-Ni

    Plasmid

    #17744

    Depositor

    Bert Vogelstein

    Article

    Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

    Insert

    PIK3R1-Ni (PIK3R1 Human)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Partial cDNA

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. mRuby2-MannII-N-10

    Plasmid

    #55903

    Purpose

    Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600

    Depositor

    Michael Davidson

    Article

    Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

    Insert

    MannII
    

    Use

    Tags

    mRuby2

    Expression

    Mammalian

    Mutation

    aa 1-112 of MannII

    Promoter

    CMV

    Available since

    March 10, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

12. ManII-SBP-EGFP_puromycin

    Plasmid

    #65260

    Purpose

    ManII reporter only (no hook) (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    truncated Mannosidae II
    

    Use

    Tags

    EGFP and Streptavidin Binding Protein (SBP)

    Expression

    Mammalian

    Mutation

    contains only amino acids 1 to 116 of ManII

    Promoter

    CMV

    Available since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDGE8

    Plasmid

    #79463

    Purpose

    sgRNA shuttle vector; module 2E

    Depositor

    Johannes Stuttmann

    Article

    Ordon et al Plant J. 2016 Aug 31. doi: 10.1111/tpj.1

    Insert

    pAtU6-ccdB-sgRNA (ccdB(letD) )
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI site eliminated

    Promoter

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGBW-m4046763

    Plasmid

    #145606

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;FLAG_2

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGBW-m4046942

    Plasmid

    #145757

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp6

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp6 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 28, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. Str-KDEL_flGalT-SBP-tagBFP

    Plasmid

    #65274

    Purpose

    synchronize trafficking of GalT from the ER (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    galactosidase
    

    Use

    Tags

    Streptavidin Binding Protein (SBP) and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCytERM-mTagBFP2

    Plasmid

    #184940

    Purpose

    ER Visualization (OSER assay) Expresses mTagBFP2 in fusion with N-terminal part of p450

    Depositor

    Kiryl Piatkevich

    Article

    Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

    Insert

    CytERM-mTagBFP2
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pETM6-At4CL-CmCHS-CmCHI

    Plasmid

    #73395

    Purpose

    Expresses three enzymes to convert phenylpropanoic acids to flavanones in vivo

    Depositor

    Mattheos Koffas

    Article

    Jones et al Metab Eng. 2016 Feb 6;35:55-63. doi: 10.

    Inserts

    At4CL
    CmCHS
    CmCHI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Dendra2-Keratin-17

    Plasmid

    #57726

    Purpose

    Note- This plasmid contains Keratin 18. Localization: Cytokeratin, Excitation: 490 / 553, Emission: 507 / 573

    Depositor

    Michael Davidson

    Article

    McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

    Insert

    Keratin (KRT18 Human)
    

    Use

    Tags

    Dendra2

    Expression

    Mammalian

    Mutation

    V282L

    Promoter

    CMV

    Available since

    Jan. 13, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

20. pcDNA3.1: TfR1-CoilE

    Plasmid

    #181988

    Purpose

    CoilE genetically encoded tag on Transferrin Receptor 1 (TfR1) to be used with CoilR labeled with a reporter

    Depositor

    Kimberly Beatty

    Article

    Doh et al Proc Natl Acad Sci U S A. 2018 Dec 18;11

    Insert

    TfR1-CoilE
    

    Use

    Tags

    CoilE

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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