162,676 results

1. AAV-P(Per2)-DIO-intron2-Venus-NLS-D2

   Plasmid

   #110057

   Purpose

   Per2 transcription fluorescence reporter

   Depositor

   Eric Erquan Zhang

   Article

   Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

   Insert

   Per2 promoter and Venus (Per2 Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   NLS-D2

   Expression

   Mammalian

   Mutation

   Promoter

   Per2

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMRXIP-GFP-PI4K2A

   Plasmid

   #221760

   Purpose

   Stable expression of GFP-PI4K2A in mammalian cells

   Depositor

   Noboru Mizushima

   Article

   Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

   Insert

   PI4K2A (PI4K2A Human)
   

   Use

   Retroviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. MZB209

   Plasmid

   #228492

   Purpose

   BirA encoded pACYC vector for co-expression with Avi-tagged constructs (Bacterial Expression)

   Depositor

   Richard Baker

   Insert

   BirA (birA )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7 Promoter

   Available since

   Jan. 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pWN_U6-TRAC-miniGag-Cas9

   Plasmid

   #228959

   Purpose

   miniEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)

   Depositor

   Jennifer Doudna

   Article

   Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

   Insert

   miniGag-Cas9
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT3-EF1a-Bmi1

   Plasmid

   #31783

   Depositor

   Xin Chen

   Article

   Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

   Insert

   Bmi1 (BMI1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-Tight-Puro-KrasG12D2B

   Plasmid

   #139786

   Purpose

   Lentiviral tet-inducible expression of KrasG12D (mouse)

   Depositor

   Joan Massague

   Article

   Su et al Nature. 2020 Jan;577(7791):566-571. doi:

   Insert

   KrasG12D (Kras Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   G12D

   Promoter

   Available since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRSET-4xTR-TUBE

   Plasmid

   #110312

   Purpose

   E. coli expression and purification of 4xTR-TUBE

   Depositor

   Yasushi Saeki

   Article

   Tsuchiya et al Nat Commun. 2018 Feb 6;9(1):524. doi: 10

   Insert

   4xTR-TUBE (UBQLN1 Human)
   

   Use

   Tags

   N-terminal His6-T7 tag

   Expression

   Bacterial

   Mutation

   All Arg residues in the UBA domain are mutated to…

   Promoter

   T7

   Available since

   May 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF-Con/Fon TVA-mCherry

   Plasmid

   #131779

   Purpose

   INTRSECT 2.0

   Depositor

   Karl Deisseroth

   Article

   Hafner et al Cell Rep. 2019 Sep 24;28(13):3450-3461.e

   Insert

   TVA-mCherry
   

   Use

   AAV; Intrsect

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   nEF

   Available since

   Sept. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. CD59-bio-His

   Plasmid

   #53154

   Purpose

   Expresses full-length CD59 ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

   Depositor

   Gavin Wright

   Article

   Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

   Insert

   CD59 (CD59 Human)
   

   Use

   Tags

   His tag, enzymatic biotinylation sequence, and ra…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-SARS-CoV-2-S-RBD-sfGFP

    Plasmid

    #141184

    Purpose

    Mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike) fused with sfGFP

    Depositor

    Erik Procko

    Article

    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.

    Has Service

    Cloning Grade DNA

    Insert

    S surface glycoprotein [ Severe acute respiratory syndrome coronavirus 2 ] (S SARS coronavirus 2)
    

    Use

    Tags

    Signal peptide from influenza HA and Superfolder …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. Anti-TrpC1 [1F1R]

    Plasmid

    #177437

    Purpose

    Mammalian Expression Plasmid of anti-TrpC1 (Human). Derived from hybridoma 1F1.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-TrpC1 (Homo sapiens) recombinant mouse monoclonal antibody (TRPC1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Nov. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA5 CMV 3xFG eIF4G1

    Plasmid

    #158775

    Purpose

    pcDNA5 transfection plasmid for the exogenous expression of N-terminal 3xFlag-tagged eIF4G1 isoform lacking the microexon.

    Depositor

    Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Mol Cell. 2020 Mar 19;77(6):1176-1192.e1

    Insert

    eIF4G1
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pExpreS2-1-C3-10H-SIII

    Plasmid

    #175444

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    3C-10His-TwinStrep and BiP Secretion Peptide

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTOX5

    Plasmid

    #127450

    Purpose

    Encodes MqsR toxin and amilCP chromoprotein. CAM-R

    Depositor

    Matthew Waldor

    Article

    Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

    Inserts

    mqsR toxin
    amilCP chromoprotein
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized from protein sequence in FPbase

    Promoter

    pRha and tac

    Available since

    July 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-Rab27A

    Plasmid

    #89237

    Purpose

    Expression of GFP-tagged human Rab27A in mammalian cells

    Depositor

    William Gahl

    Article

    Westbroek et al Mol Genet Metab. 2008 Jun;94(2):248-54.

    Insert

    human Rab27A (RAB7A Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    wild type

    Promoter

    CMV

    Available since

    April 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

    Plasmid

    #52092

    Purpose

    Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

    Inserts

    CD3 delta (Cd3d Mouse)
    CDC gamma (Cd3g Mouse)
    CD3 epsilon (Cd3e Mouse)
    CD3 zeta (Cd247 Mouse)
    

    Use

    Retroviral

    Tags

    F2A, IRES-EGFP, P2A, and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. PET-Sniper SpCas9-NLS-6xHis

    Plasmid

    #207374

    Purpose

    Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

    Insert

    Sniper SpCas9
    

    Use

    CRISPR

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F539S, M763I, K890N

    Promoter

    T7

    Available since

    Sept. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-CBE4max-SpRYc-P2A-EGFP

    Plasmid

    #208336

    Purpose

    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpRYc

    Depositor

    Pranam Chatterjee

    Article

    Zhao et al Nat Commun. 2023 Oct 4;14(1):6175. doi:

    Insert

    human codon optimized CBE4max Cas9 variant named SpRYc with P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Sc++ (1-1119), SpRY (1111-1368), D10A

    Promoter

    CAG

    Available since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. ATG9A-mCherry

    Plasmid

    #197393

    Purpose

    Expresses ATG9A in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    De Pace et al PLoS Genet. 2018 Apr 26;14(4):e1007363.

    Insert

    ATG9A (ATG9A Human)
    

    Use

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFCiKm7

    Plasmid

    #167521

    Purpose

    Broad-Host range expression vector, RSF1010 origin, CinRAM activator, PCin promoter, KmR, Erythromycin Resistance Marker.

    Depositor

    Christopher Reisch

    Article

    Schuster et al Nucleic Acids Res. 2021 Jun 14. pii: 629

    Insert

    CinRAM-KmR
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PCin

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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