We narrowed to 9,980 results for: Uty

1. nDHFR173-gs10-NLS-VPR

   Plasmid

   #188262

   Purpose

   Plasmid ensures constitutive expression of the N-terminal fragment of split DHFR (aa 174-186), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

   Depositor

   Roman Jerala

   Article

   Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

   Insert

   nDHFR173 (DHFR Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. J23117-mRFP1-331Bb

   Plasmid

   #78280

   Purpose

   mRFP1 behind constitutive promoter J23117 in pSEVA331Bb backbone

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Insert

   J23117-mRFP1
   

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCEP4-myc-HLA-A2-VN

   Plasmid

   #135485

   Purpose

   Mammalian expression of VN-fused and myc-tagged HLA-A*02:01

   Depositor

   Erik Procko

   Article

   McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

   Insert

   HLA-A*02:01 (HLA-A Human)
   

   Tags

   Extracellular c-myc epitope tag, Signal peptide f…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-4T-1 3xHA-MKK7a1-EE

   Plasmid

   #47578

   Purpose

   Bacterial expression plasmid for GST-3xHA-tagged constitutively active MKK7a1

   Depositor

   Kevin Janes

   Article

   Bose et al Mol Cell Proteomics. 2013 Mar;12(3):797-

   Insert

   MKK7a1-EE (Map2k7 Mouse)
   

   Tags

   3xHA and GST

   Expression

   Bacterial

   Mutation

   Residues 73-420 of RefSeq sequence. Ser271 to Gl…

   Available Since

   Aug. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pEGB3 alpha1 Pnos:PhiC31int:Tnos (GB1531)

   Plasmid

   #160616

   Purpose

   TU for the constitutive expression of Streptomyces phage PhiC31 integrase.

   Depositor

   Diego Orzaez

   Article

   Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379

   Insert

   PhiC31
   

   Use

   Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Pnos

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMIR-CDH1 mutUTR

   Plasmid

   #25039

   Depositor

   Bob Weinberg

   Article

   Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

   Insert

   CDH1 mutant 3'UTR (CDH1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   substitution of 6nt within the miR-9 binding site…

   Available Since

   June 7, 2010

   Availability

   Academic Institutions and Nonprofits only

7. pCI-neo-(HA)3-ε

   Plasmid

   #198531

   Purpose

   Expression of HA-tagged AP-4 ε in mammalian cells

   Depositor

   Juan Bonifacino

   Article

   Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

   Insert

   AP-4 ε (AP4E1 Human)
   

   Tags

   triple HA tag

   Expression

   Mammalian

   Mutation

   silent substitutions in codons 235, 262, 905 and …

   Promoter

   CMV

   Available Since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. J23114-mRFP1-331Bb

   Plasmid

   #78279

   Purpose

   mRFP1 behind constitutive promoter J23114 in pSEVA331Bb backbone

   Depositor

   Tom Ellis

   Article

   Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

   Insert

   J23114-mRFP1
   

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMV-REX-GECO0.9

   Plasmid

   #61247

   Purpose

   Expresses REX-GECO0.9 in mammalian cells

   Depositor

   Robert Campbell

   Article

   Wu et al Nat Commun. 2014 Oct 31;5:5262. doi: 10.

   Insert

   REX-GECO0.9
   

   Expression

   Mammalian

   Mutation

   Substitutions relative to R-GECO1: P60R, V61W, R6…

   Promoter

   CMV

   Available Since

   March 27, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pAC8_MF-PH-HA-Cter (VE5629)

    Plasmid

    #139775

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter HA tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal Hemaglutinine tag
    

    Tags

    HA

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

    Plasmid

    #140227

    Purpose

    Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    HRE (Hypoxia inducible)

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. CFP-Rpp29-C1

    Plasmid

    #134546

    Purpose

    Expresses CFP-Rpp29 fusion protein

    Depositor

    Susan Janicki

    Article

    Newhart et al Mol Biol Cell. 2016 Apr 1;27(7):1154-69.

    Insert

    Rpp29 (POP4 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCEP4-myc-HLA-A3-VN

    Plasmid

    #135506

    Purpose

    Mammalian expression of VN-fused and myc-tagged HLA-A*03:01

    Depositor

    Erik Procko

    Article

    McShan et al Proc Natl Acad Sci U S A. 2019 Dec 17;11

    Insert

    HLA-A*03:01 (HLA-A Human)
    

    Tags

    Extracellular c-myc epitope tag, Signal peptide f…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTSara-R756K

    Plasmid

    #60727

    Purpose

    Expresses both fragments of T7 RNAP split at position 179. Both fragments are driven by PBAD. The C-terminal fragment has the point mutation R756K. Contains a constitutive araC ORF

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Inserts

    Residues 1-179 of split T7 RNAP
    Residues 180-880 of split T7 RNAP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    Residues 1-180 of split T7 RNAP and Residues 180-…

    Available Since

    Oct. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA6-E2F4CA-Myc

    Plasmid

    #80428

    Purpose

    Mammalian expression of constitutively active chick E2F4

    Depositor

    José María Frade

    Article

    Morillo et al Mol Cell Biol. 2012 Jul;32(14):2722-37.

    Insert

    E2F transcription factor 4 (E2F4 Chicken)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    T261E, T263E

    Promoter

    CMV

    Available Since

    July 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET14b-mEKLF

    Plasmid

    #67834

    Purpose

    Encodes murine EKLF (nucleotides 71-1215) for in vitro chromatin/transcription reconstitution

    Depositor

    James Bieker

    Article

    Armstrong et al Cell. 1998 Oct 2;95(1):93-104.

    Insert

    EKLF (Klf1 Mouse)
    

    Expression

    Bacterial

    Mutation

    Full-length mEKLF is aa 20-376; amino acid 19 is …

    Promoter

    T7

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

17. BMR1_02g00320-bio-His

    Plasmid

    #108028

    Purpose

    Expresses enzymatically monobiotinylated full-length BMR1_02g00320 ectodomain with no N-linked glycans upon cotransfection with BirA in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag.

    Depositor

    Gavin Wright

    Article

    Elton et al Int J Parasitol. 2019 Feb;49(2):115-125.

    Insert

    BMR1_02g00320
    

    Tags

    ratCD4d3+4,biotinylation sequence, 6xHis

    Expression

    Mammalian

    Mutation

    all potential N-linked glycosylation sites (N-X-S…

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. M2E-1

    Plasmid

    #21206

    Depositor

    Paul Khavari

    Article

    Scholl et al Cancer Res. 2004 Sep 1;64(17):6035-40

    Insert

    Mek2 (Esr1, MAP2K2 Mouse, Human)
    

    Use

    Subclone vector

    Mutation

    deletion of residues EQQKKRLE, S222D S226D in Mek…

    Available Since

    July 24, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pSLIK-Venus-TmiR-G13-G12

    Plasmid

    #25742

    Purpose

    Lentiviral vector with Tet-based inducible expression of both mouse G alpha 12 and G alpha 13 miR30-based shRNAs, constitutive Venus fluorescent protein coexpression.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 13 and 12 miR-shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Venus

    Expression

    Mammalian

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. J23110-mRFP1-331Bb

    Plasmid

    #78277

    Purpose

    mRFP1 behind constitutive promoter J23110 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23110-mRFP1
    

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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