We narrowed to 9,182 results for: Pol

1. pVD1 M1_3-pTRNA-scf 2.1 (GB2073)

   Plasmid

   #160558

   Purpose

   tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M1_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   M1_3-pTRNA-scf 2.1
   

   Use

   CRISPR

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pVD1 M2_3-pTRNA-scf 2.1 (GB2074)

   Plasmid

   #160559

   Purpose

   tRNA and scaffold 2.1scRNA aptamer Ms2 for the assembly of GBoligomers for the first position (positon [M2_3]) of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   M2_3-pTRNA-scf 2.1
   

   Use

   CRISPR

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Feb. 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. tRNA-gRNA position [M1_2] (GB1209)

   Plasmid

   #75410

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_2]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (3-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA position [M1_2]
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - C130S, E446C

   Plasmid

   #60394

   Purpose

   Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) C130S, E446C for crosslinking polyglutamate peptide

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB1 (TUB1 Budding Yeast, Synthetic)
   

   Expression

   Yeast

   Mutation

   internal 6xHis (see supplement figure 4), amino a…

   Promoter

   GAL

   Available Since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pFL-EGFP-3xFlag-HLTF

   Plasmid

   #193055

   Purpose

   Baculovirus expression of human HLTF for recombinant protein production

   Depositor

   Andrew Deans

   Article

   Hodson et al Cell Rep. 2022 Dec 6;41(10):111749. doi:

   Insert

   HLTF (HLTF Human)
   

   Tags

   3xFlag

   Expression

   Insect

   Promoter

   polyhedron

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28a-MH6-Bsu

   Plasmid

   #163911

   Purpose

   Expresses Bsu large fragment for affinity purification

   Depositor

   Paul Freemont

   Article

   Patchsung et al CRISPR J. 2022 Nov 11. doi: 10.1089/cris

   Insert

   Bsu polymerase
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   March 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTol2-HuC(elavl3)-CaMPARI2

   Plasmid

   #137185

   Purpose

   pan-neuronal expression of CaMPARI2 in zebrafish, used to generate the CaMPARI2 zebrafish line at ZIRC (ZL13801)

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Insert

   HuC(elavl3)-CaMPARI2-FLAG-HA-myc-polyA
   

   Use

   Tol2 plasmid for zebrafish

   Tags

   FLAG, HA, myc

   Promoter

   HuC(elavl3)

   Available Since

   March 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pSSRB069_pAC8-hsDDB1dB

   Plasmid

   #218790

   Purpose

   Insect cell expression vector for 6xHis-TEV-hsDDB1∆B

   Depositor

   Eric Fischer

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   DNA damage-binding protein 1 (DDB1 Human)
   

   Tags

   6xHis-TEV

   Expression

   Insect

   Mutation

   Deleted amino acids 396-705 and replaced with GNG…

   Promoter

   polyhedrin

   Available Since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pFastBac-M13-6His-KIF1C-GFP

   Plasmid

   #130975

   Purpose

   Expression of 6His-KIF1C-GFP in insect cells for protein purification.

   Depositor

   Anne Straube

   Article

   Siddiqui et al Nat Commun. 2019 Jun 19;10(1):2693. doi:

   Insert

   KIF1C-GFP (KIF1C Human)
   

   Tags

   6His and GFP

   Expression

   Insect

   Mutation

   5 silent mutations that make this construct RNAi …

   Promoter

   Polyhedrin

   Available Since

   Oct. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFastBac-GFP-dynein-2(D1091–Q4307)

    Plasmid

    #64064

    Purpose

    Codon-optimized human dynein-2 motor domain (D1091–Q4307) for baculovirus expression. 6His-ZZ-GFP N-terminal tag, TEV site to cleave 6His-ZZ.

    Depositor

    Andrew Carter

    Article

    Schmidt et al Nature. 2015 Feb 19;518(7539):435-8. doi

    Insert

    Cytoplasmic dynein-2 (DYNC2H1 Human)
    

    Tags

    GFP, His tag, and ZZ tag

    Expression

    Insect

    Mutation

    Deleted amino acids 1-1090, extra C-terminal vali…

    Promoter

    Polyhedrin

    Available Since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSSRB274_pAC8-eGFP-hsCRBN

    Plasmid

    #218791

    Purpose

    Insect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBN

    Depositor

    Eric Fischer

    Article

    Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

    Insert

    Protein cereblon (CRBN Human)
    

    Tags

    FLAG-TEV-eGFP-Prescission

    Expression

    Insect

    Mutation

    eGFP fused to N-terminus

    Promoter

    polyhedrin

    Available Since

    May 6, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGHUC w-ERBIN (2-hybrid prey plasmid)

    Plasmid

    #128163

    Purpose

    UV5 promoter drives expression of omega-erbin fusion. Pair with pB1H2 UV5 Zif268-cons as a positive control (turns on HIS3/GFP).

    Depositor

    Marcus Noyes

    Article

    Ichikawa et al ACS Synth Biol. 2019 May 17;8(5):918-928

    Insert

    ERBIN PDZ domain (ERBIN Synthetic)
    

    Use

    Synthetic Biology

    Tags

    FLAG and Omega subunit of RNA polymerase

    Expression

    Bacterial

    Promoter

    UV5

    Available Since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pEW108

    Plasmid

    #232823

    Purpose

    Expresses yeast compass complex in insect cells

    Depositor

    Cynthia Wolberger

    Article

    Worden et al Elife. 2020 Jan 10;9. pii: 53199. doi: 1

    Insert

    COMPASS (Cps60, Cps50, Cps35, Cps25, Cps15, Flag-Set1, Cps30, Twinstrep-Cps40) (BRE2, SWD1, SET1, SPP1, SWD3, SWD2, SDC1, SHG1 Budding Yeast)
    

    Tags

    His6-3xFLAG, Twinstrep

    Expression

    Insect

    Mutation

    Set1(762-1080)

    Promoter

    polyhedrin promoter

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSN840

    Plasmid

    #184832

    Purpose

    Expresses human KIF5A(∆exon27) and human KLC1 in insect cells

    Depositor

    Kyoko Chiba

    Article

    Nakano et al Genes Cells. 2022 Apr 17. doi: 10.1111/g

    Inserts

    KIF5A (∆exon27) (KIF5A Human)
    KLC1 (KLC1 Human)
    

    Use

    Cre/Lox

    Tags

    His-FLAG and mScarlet-2xStrepII

    Expression

    Insect

    Mutation

    ∆exon27 form of human KIF5A

    Promoter

    p10 and polyhedrin

    Available Since

    July 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGS_101

    Plasmid

    #160541

    Purpose

    Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

    Depositor

    Li-Huei Tsai, Susan Lindquist

    Article

    Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

    Insert

    Apolipoprotein E e4 (APOE Human)
    

    Tags

    Kar2 signal sequence

    Expression

    Yeast

    Mutation

    e4 allele

    Promoter

    GAL1

    Available Since

    Jan. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pACEBac1-POMP-PSMG1-PSMG2-PSMG3-PSMG4

    Plasmid

    #214137

    Purpose

    Insect cell expression of the human 20SCP proteasome assembly chaperones

    Depositor

    Brenda Schulman

    Article

    Adolf et al Nat Struct Mol Biol. 2024 Apr 10. doi: 1

    Inserts

    POMP (POMP Human)
    PSMG1 (PSMG1 Human)
    PSMG2 (PSMG2 Human)
    PSMG3 (PSMG3 Human)
    PSMG4 (PSMG4 Human)
    

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. GFP-Myo10

    Plasmid

    #135403

    Purpose

    Expresses GFP-tagged bovine Myo10 in mammalian cells.

    Depositor

    Richard Cheney

    Article

    Berg et al Nat Cell Biol. 2002 Mar;4(3):246-50. doi

    Insert

    Myo10 (MYO10 Bovine)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    "Relative to the GenBank Myo10 cDNA sequence…

    Promoter

    CMV

    Available Since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHIV-EGFP.Flag-EZH2-mt2

    Plasmid

    #220245

    Purpose

    Expresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.

    Depositor

    Chen Davidovich

    Article

    Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

    Insert

    EZH2 mt 2 (EZH2 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

    Promoter

    EF-1α

    Available Since

    June 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pVD1 tRNA-gRNA position E2 (GB2239)

    Plasmid

    #160561

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for position [2-3] of a polycistronic tRNA-gRNA

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    tRNA-gRNA position E2 (Multiplexing Edit)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Actc1

    Plasmid

    #99690

    Purpose

    Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Actc1, vector allows for strong activation of mouse Actc1, can be packaged and delivered as AAV

    Depositor

    George Church

    Article

    Vora et al bioRxiv 2018. 298620

    Insert

    dCas9 and gRNA targeting Actc1 (gRNA sequence: GCCATTCTTGGAGCCAAGGG ) (Actc1 Synthetic, Mouse)
    

    Use

    AAV and CRISPR

    Tags

    VPR mini

    Mutation

    dead Cas9

    Available Since

    Sept. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge