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HA-MBP-TEVpCS-ADGRF2 CTFdeltaTA-FLAG

Plasmid

#217728

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRF2 (ADGRF2 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-435

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG3 CTF-FLAG

Plasmid

#217701

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG3 (ADGRG3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-249

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRG3 CTFdeltaTA-FLAG

Plasmid

#217734

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al Cell Chem Biol. 2024 Aug 15;31(8):1542-1

Insert

ADGRG3 (ADGRG3 Human)

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-255

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pS12<ActB.mus_2A-mCh_KI-HMEJ

Plasmid

#207407

Purpose

Donor template to knock in mCherry at the C-terminus of mouse ActB (β-Actin) using DSB repair by homology-mediated end joining (HMEJ). [Lab plasmid ID: TU148]

Depositor

Alexandros Poulopoulos

Article

Richardson et al CRISPR J. 2023 Oct;6(5):447-461. doi: 10

Insert

Actb (Actb Mouse)

Use

CRISPR and Mouse Targeting; Hmej knockin donor

Tags

mCherry

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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CL20_mEGFP_CSF2RA_CRLF2

Plasmid

#205798

Purpose

Express mEGFP-tagged fusion protein, CSF2RA_CRLF2 from patient-derived sequence

Depositor

Richard Kriwacki

Article

Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

Insert

CSF2RA_CRLF2 (CSF2RA, CRLF2 Human)

Tags

mEGFP

Expression

Mammalian

Available Since

Nov. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-mNeonGreen-NUT

Plasmid

#204693

Purpose

This plasmid allows for inducible expression of full-length NUT tagged with mNeonGreen, in mammalian cells.

Depositor

Michael Rosen

Article

Kosno et al Sci Rep. 2023 Jul 24;13(1):11907. doi: 1

Insert

NUT (NUTM1 Human)

Tags

mNeonGreen

Expression

Mammalian

Mutation

n/a

Promoter

CMV

Available Since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Tom70NTD-ABHD16Acyto-mNG

Plasmid

#195159

Purpose

mammalian expression of outer mitochondrial membrane localized ABHD16A tagged with mNeonGreen

Depositor

Gia Voeltz

Article

Nguyen et al Elife. 2022 Nov 30;11:e84279. doi: 10.75

Insert

Tom70NTD (TOMM70 Human)

Tags

ABHD16A cytoplasmic domain and mNeonGreen

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-ZNF385A

Plasmid

#101615

Purpose

Donor Vector containing ZNF385A transcription factor, part of the Human TFome Collection

Depositor

George Church

Article

Ng et al Nat Biotechnol. 2021 Apr;39(4):510-519.

Insert

ZNF385A (ZNF385A Human)

Use

Gateway Cloning

Mutation

Last nucleotide of stop codon removed to allow fo…

Available Since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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C239-E02: attB2r-IRES-ffLuc2-eGFP-pA-attB3

Plasmid

#162918

Purpose

Gateway attB2r/attB3 entry clone for generation of polycistronic ffLuc2-eGFP reporter using an internal ribosome entry sequence (IRES)

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

firefly luciferase/green fluorescent protein fusion with upstream IRES sequence

Use

Synthetic Biology

Available Since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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Tmem98-mTq2

Plasmid

#124504

Purpose

Expresses mTurquoise2-tagged mouse TMEM98 in mammalian cells (C-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Tmem98 (Tmem98 Mouse)

Tags

mTurquoise2

Expression

Mammalian

Mutation

stop codon was removed to allow fusion with sGFP2…

Promoter

CMV

Available Since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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Tmem98-mCherry

Plasmid

#124505

Purpose

Expresses mCherry-tagged mouse TMEM98 in mammalian cells (C-terminal tag)

Depositor

Renee van Amerongen

Article

van der Wal et al PLoS One. 2020 Jan 21;15(1):e0227435. do

Insert

Tmem98 (Tmem98 Mouse)

Tags

mCherry

Expression

Mammalian

Mutation

stop codon was removed to allow fusion with sGFP2…

Promoter

CMV

Available Since

May 12, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:FRB:nls:VPR

Plasmid

#135987

Purpose

Expresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

FRB domain fused to the VPR activation domain

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Flex-rev-GFPFANACfullWT

Plasmid

#126551

Purpose

AAV Flex reverse plasmid with the coding sequence of eGFP fused to the N-term of the FMRFamide gated Na channel

Depositor

Rory McQuiston

Article

Schanuel et al Neuroscience. 2008 Aug 13;155(2):374-86.

Insert

eGFP N-terminal fused to FMRFamide gate sodium channel

Use

AAV

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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hRad51(F86E)–Cas9(D10A)

Plasmid

#125564

Purpose

Expresses the Rad51(F86E)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(F86E)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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hRad51(G151D)–Cas9(D10A)

Plasmid

#125565

Purpose

Expresses the Rad51(G151D)–Cas9(D10A) fusion construct in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51(G151D)–Cas9(D10A) (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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LLP250 pEF1a-TALE-SunTag

Plasmid

#100939

Purpose

Empty TALE vector fused to SunTagx10 driven by EF1a promoter, with 3xHA tag, no PURO gene

Depositor

Ryan Lister

Article

Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

Insert

TALE insertion site, SunTag array (10xGCN4)

Use

TALEN

Tags

3xHA

Expression

Mammalian

Available Since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

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pmEos2-Linker_PCNA-MutC

Plasmid

#98272

Purpose

for low level expression of mEos2-PCNA in mammalian cells

Depositor

Mike Heilemann

Article

Zessin et al Sci Rep. 2016 Jan 13;6:18779. doi: 10.10

Insert

PCNA (PCNA Human)

Tags

mEos2

Expression

Mammalian

Mutation

Silent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…

Promoter

CMV

Available Since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMXs-Puro-DUS2L-PSKH1

Plasmid

#69472

Purpose

Expresses DUS2L-PSKH1 fusion gene

Depositor

Axel Hillmer

Article

Yao et al Cell Rep. 2015 Jul 14;12(2):272-85. doi:

Insert

DUS2L-PSKH1 (PSKH1 Human)

Use

Retroviral

Tags

HA TAG

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pLacIBD::SDRX (GB0673)

Plasmid

#68183

Purpose

Provides the LacI Binding Domain fused to SRDX Transcriptional Repressor as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

LacI Binding Domain fused to SRDX Transcriptional Repressor

Use

Synthetic Biology

Mutation

BsaI and BsmBI sites removed

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pCSDest2-2XNLS-SpCas9-WT-NLS-3XHA-NLS-TALentry

Plasmid

#69232

Purpose

Wild type SpCas9 expression plasmid to clone TALEs through BbsI site (generates compatible overhangs with Acc65I and BamHI sites)

Depositor

Scot Wolfe

Article

Bolukbasi et al Nat Methods. 2015 Oct 19. doi: 10.1038/n

Insert

SpCas9

Use

CRISPR

Tags

2X NLS, BbsI TALE entry cassette, and NLS-3XHA-NL…

Expression

Mammalian

Available Since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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