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pSH-EFIRES-P-Seipin-miniIAA7-3XFlag

Plasmid

#129722

Purpose

AAVS1 safe harbor targeting vector expressing miniIAA7-3XFlag tagged Seipin

Depositor

Elina Ikonen

Publication

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

Seipin (BSCL2 Human)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

miniIAA7-3XFlag

Expression

Mammalian

Mutation

silent mutations

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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FDP-P10B2-A75-RZ-URA3

Plasmid

#130874

Purpose

Integration cassette for OrthoRep containing mKate driven by promoter 10B2 with a hardcoded polyA tail

Depositor

Chang Liu

Publication

Zhong et al ACS Synth Biol. 2018 Dec 21;7(12):2930-2

Insert

mKate

Use

Expression of mkate, leu2(q180*), and ura3 on ort…

Available since

Aug. 21, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p23-DDX21 WT

Plasmid

#128803

Purpose

overexpression of DDX21 in human cells

Depositor

Ling-Ling Chen

Publication

Xing et al Cell. 2017 May 4;169(4):664-678.e16. doi

Insert

DDX21 WT

Tags

flag

Expression

Mammalian

Available since

Aug. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pH2B-miRFP670nano

Plasmid

#127438

Purpose

Histone 2B labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Publication

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

H2B-miRFP670nano (H2BC11 Synthetic, Human)

Tags

miRFP670nano

Expression

Mammalian

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCMV-BI

Plasmid

#126475

Purpose

High-copy number vector for the co-expression of two genes in mammalian cells

Depositor

Bill Hansson

Publication

Miazzi et al Chem Senses. 2019 Oct 26;44(9):673-682.

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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Halo-MTS

Plasmid

#124315

Purpose

Expresses mitochondria specific HaloTag protein

Depositor

Jin Wang

Publication

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Tags

Mito specific seq

Expression

Mammalian

Promoter

CMV

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3-ExRai-CKAR

Plasmid

#118409

Purpose

cpGFP-based excitation-ratiometric biosensor for monitoring Protein Kinase C activity.

Depositor

Jin Zhang

Publication

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

ExRai-CKAR

Tags

6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…

Expression

Mammalian

Promoter

CMV

Available since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pHAGE-ERBB3

Plasmid

#116735

Purpose

Lentiviral expression of ERBB3

Depositor

Gordon Mills, Kenneth Scott

Publication

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

ERBB3 (ERBB3 Human)

Use

Lentiviral

Mutation

WT

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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NLS-MCP-AP_pLX304

Plasmid

#120918

Purpose

Lentiviral vector expressing NLS-MCP-AP in mammalian nucleus

Depositor

Alice Ting

Publication

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

NLS-MCP-AP

Use

Lentiviral

Expression

Mammalian

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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CD40L-SrtA

Plasmid

#121167

Purpose

pMP71 vector expressing Tomato - P2A - CD40L - SrtA - flag

Depositor

Gabriel Victora

Publication

Pasqual et al Nature. 2018 Jan 25;553(7689):496-500. d

Insert

CD40L-SrtA

Use

Retroviral

Tags

Tomato and flag

Expression

Mammalian

Available since

Feb. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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alpha Synuclein-VC

Plasmid

#89471

Purpose

expresses human alpha Synuclein fused to the C-terminal part of Venus

Depositor

Bradley Hyman, Tiago Outeiro

Publication

Outeiro et al PLoS One. 2008 Apr 2;3(4):e1867. doi: 10

Insert

SNCA (SNCA Human)

Tags

C-terminal part of Venus

Expression

Mammalian

Promoter

CMV

Available since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pmGFP

Plasmid

#117926

Purpose

Control plasmid expressing myc-GFP.

Depositor

Kumiko Ui-Tei

Publication

Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

Type

Empty backbone

Expression

Mammalian

Available since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pFL-SV40

Plasmid

#115352

Purpose

firefly luciferase (Fluc) reporter gene driven by SV40 promoter

Depositor

Ming-Chih Lai

Publication

Lai et al Mol Biol Cell. 2008 Sep;19(9):3847-58. d

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pGPA1

Plasmid

#115476

Purpose

Generation of mariner-based transposon mutant libraries in Enterococcus faecium

Depositor

Willem van Schaik

Publication

Zhang et al BMC Genomics. 2017 Nov 21;18(1):893. doi

Type

Empty backbone

Expression

Bacterial

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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7a sgRNA for EJ7-GFP and 4-μHOM reporters

Plasmid

#113620

Purpose

sgRNA/CAS9 expression plasmid to induce the 5’ double-strand break in both the EJ7-GFP and 4-μHOM reporters

Depositor

Jeremy Stark

Publication

Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

Insert

7a sgRNA

Use

CRISPR

Expression

Mammalian

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles
EasyFusion Halo

Plasmid

#112850

Purpose

General donor vector with HaloTag coding sequence flanking by restriction sites for generating knock-in donor

Depositor

Janet Rossant

Publication

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

HaloTag

Use

Synthetic Biology

Promoter

No

Available since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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AAV-P(Per2)-DIO-intron2-dLUC

Plasmid

#110059

Purpose

Per2 transcription luciferase reporter

Depositor

Eric Erquan Zhang

Publication

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and luciferase (Per2 Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Per2

Available since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

Plasmid

#100834

Purpose

AAV expression of ultrasensitive protein calcium sensor from CamKII promoter

Depositor

James M. Wilson

Has service

AAV1, AAV5, and AAV9

Insert

GCaMP6f

Use

AAV

Expression

Mammalian

Mutation

GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

Promoter

CamKII

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

80 Articles

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pX-evoCas9

Plasmid

#107550

Purpose

Expresses the M495V/Y515N/K526E/R661Q (evoCas9) SpCas9 variant in mammalian cells

Depositor

Anna Cereseto

Publication

Casini et al Nat Biotechnol. 2018 Jan 29. pii: nbt.40

Insert

Humanized S. pyogenes Cas9

Use

CRISPR

Tags

3XFLAG

Expression

Mammalian

Mutation

M495V/Y515N/K526E/R661Q

Promoter

CBh

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pHis-hTG2

Plasmid

#100719

Purpose

Expression of human transglutaminase 2 in E.coli

Depositor

Byung Il Lee

Publication

Han et al Int J Biol Macromol. 2010 Aug 1;47(2):19

Insert

TGM2 (TGM2 Human)

Tags

His

Expression

Bacterial

Mutation

silent mutation Arg580 (AGG--CGT) to enhance expr…

Promoter

T7

Available since

Nov. 9, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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